Patterns of methyl and O-acetyl esterification in spinach pectins

Perrone, Paola; Hewage, Chandralal M.; Thomson, Adam R.; Bailey, Kevin; Sadler, Ian H.; Fry, Stephen C.

doi:10.1016/s0031-9422(02)00039-0

Cited by 133 publications

(64 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HGA is a linear chain of 1,4-α-galacturonic acid (GalA) residues in which some of the carboxyl groups are methyl-esterified. Depending on the plant source, HGA may also be partially O-acetylated (Ishii 1997, Perrone et al 2002. RGI is a branched heteropolymer of alternating 1,2-α-rhamnose and 1,4-α-GalA residues that carries neutral side-chains of arabinan, galactan or arabinogalactan attached to the rhamnose residues of the RGI backbone.…”

Section: Introductionmentioning

confidence: 99%

In vivo Expression of a Cicer arietinum β-galactosidase in Potato Tubers Leads to a Reduction of the Galactan Side-chains in Cell Wall Pectin

Martín

Dopico

Muñoz

et al. 2005

Plant and Cell Physiology

View full text Add to dashboard Cite

We report the generation of Solanum tuberosum transformants expressing Cicer arietinum βIII-Gal. βIII-Gal is a β-galactosidase able to degrade cell wall pectins during cell wall loosening that occurs prior to cell elongation. cDNA corresponding to the gene encoding this protein was identified among several chickpea β-galactosidase cDNAs, and named CanBGal-3. CanBGal-3 cDNA was expressed in potato under the control of the granule-bound starch synthase promoter. Three βIII-Gal transformants with varying levels of expression were chosen for further analysis. The transgenic plants displayed no significant altered phenotype compared to the wild type. However, β-galactanase and β-galactosidase activities were increased in the transgenic tuber cell walls and this affected the potato tuber pectins. A reduction in the galactosyl content of up to 50% compared to the wild type was observed in the most extreme transformant, indicating a reduction of 1,4-β-galactan side-chains, as revealed by analysis with LM5 specific antibodies. Our results confirm the notion that the pectin-degrading activity of chickpea βIII-Gal reported in vitro also occurs in vivo and in other plants, and confirm the involvement of βIII-Gal in the cell wall autolysis process. An increase in the homogalacturonan content of transgenic tuber cell walls was also observed by Fourier transform infrared spectroscopy (FTIR) analysis.

show abstract

Section: Introductionmentioning

confidence: 99%

In vivo Expression of a Cicer arietinum β-galactosidase in Potato Tubers Leads to a Reduction of the Galactan Side-chains in Cell Wall Pectin

Martín

Dopico

Muñoz

et al. 2005

Plant and Cell Physiology

View full text Add to dashboard Cite

show abstract

“…The plant cell wall is mainly composed of highly dynamic heteropolysaccharides assembled in macromolecular Besides the diversity of monosaccharide composition, cell wall polysaccharides are also modified with methyl, acetyl, and feruloyl groups, which are mainly O-linked to sugars. These functional groups are believed to protect polysaccharides from the action of specific glycosyl hydrolases and to cross link cell wall constituents controlling cell extensibility (Perrone et al, 2002;Gou et al, 2012). For example, methylesterification/…”

mentioning

confidence: 99%

“…These functional groups are believed to protect polysaccharides from the action of specific glycosyl hydrolases and to cross link cell wall constituents controlling cell extensibility (Perrone et al, 2002;Gou et al, 2012). For example, methylesterification/ deesterification acts in the control of cell growth, fruit ripening, and plant protection against biotic stresses (Koch and Nevins, 1989;Wolf et al, 2009;Lionetti et al, 2012).…”

mentioning

confidence: 99%

Arabidopsis and Brachypodium distachyon Transgenic Plants Expressing Aspergillus nidulans Acetylesterases Have Decreased Degree of Polysaccharide Acetylation and Increased Resistance to Pathogens

et al. 2013

View full text Add to dashboard Cite

The plant cell wall has many significant structural and physiological roles, but the contributions of the various components to these roles remain unclear. Modification of cell wall properties can affect key agronomic traits such as disease resistance and plant growth. The plant cell wall is composed of diverse polysaccharides often decorated with methyl, acetyl, and feruloyl groups linked to the sugar subunits. In this study, we examined the effect of perturbing cell wall acetylation by making transgenic Arabidopsis (Arabidopsis thaliana) and Brachypodium (Brachypodium distachyon) plants expressing hemicellulose-and pectin-specific fungal acetylesterases. All transgenic plants carried highly expressed active Aspergillus nidulans acetylesterases localized to the apoplast and had significant reduction of cell wall acetylation compared with wild-type plants. Partial deacetylation of polysaccharides caused compensatory up-regulation of three known acetyltransferases and increased polysaccharide accessibility to glycosyl hydrolases. Transgenic plants showed increased resistance to the fungal pathogens Botrytis cinerea and Bipolaris sorokiniana but not to the bacterial pathogens Pseudomonas syringae and Xanthomonas oryzae. These results demonstrate a role, in both monocot and dicot plants, of hemicellulose and pectin acetylation in plant defense against fungal pathogens.The cell wall is one of the most important compartments of the plant cell. This external cell skeleton plays an important role in cell and tissue shape determination. Besides its structural role, this extracellular complex is involved in the control of important functions such as cell-cell interactions, whole-plant growth, development, and interaction with the environment. The plant cell wall is mainly composed of highly dynamic heteropolysaccharides assembled in macromolecular Besides the diversity of monosaccharide composition, cell wall polysaccharides are also modified with methyl, acetyl, and feruloyl groups, which are mainly O-linked to sugars. These functional groups are believed to protect polysaccharides from the action of specific glycosyl hydrolases and to cross link cell wall constituents controlling cell extensibility (Perrone et al., 2002;Gou et al., 2012). For example, methylesterification/

show abstract

“…DAc ≈ 35 for sugar beet pectins (Rombouts, & Thibault, 1986). It has been reported that GalA residues in the RG-I region are partially acetylated (Ishii, 1997;Perrone, Hewage, Thomson, Bailey, Sadler, & Fry, 2002) but not methylated (Komalavilas, & Mort, 1989;Perrone, et. al., 2002).…”

Section: Introductionmentioning

confidence: 95%

A copolymer analysis approach to estimate the neutral sugar distribution of sugar beet pectin using size exclusion chromatography

Morris

Ralet

2012

Carbohydrate Polymers

View full text Add to dashboard Cite

Highlightsacid extracted sugar beet pectin is heterogeneous in terms of composition the neutral sugar composition of a pectin molecule varies with elution volume high molar mass fractions may be the result of diferulic acid bridging 3 AbstractPartially degraded sugar beet (Beta vulgaris) pectins were characterised in terms of galacturonic acid, neutral sugar and ferulic acids contents. It was shown that the total neutral sugar content is correlated with the ferulic acid content. One pectin (C) was further characterised by size exclusion chromatography coupled to refractive index and UV detectors (SEC-RI-UV). This gave the opportunity to estimate how the ferulic acid and neutral sugar contents changed with hydrodynamic radius. Pectin C was found to be heterogeneous in composition with neutral sugar-rich fractions of both high and low hydrodynamic radii. A neutral sugar-poor fraction was found at intermediate hydrodynamic radii.

show abstract

Patterns of methyl and O-acetyl esterification in spinach pectins

Cited by 133 publications

References 28 publications

In vivo Expression of a Cicer arietinum β-galactosidase in Potato Tubers Leads to a Reduction of the Galactan Side-chains in Cell Wall Pectin

In vivo Expression of a Cicer arietinum β-galactosidase in Potato Tubers Leads to a Reduction of the Galactan Side-chains in Cell Wall Pectin

Arabidopsis and Brachypodium distachyon Transgenic Plants Expressing Aspergillus nidulans Acetylesterases Have Decreased Degree of Polysaccharide Acetylation and Increased Resistance to Pathogens

A copolymer analysis approach to estimate the neutral sugar distribution of sugar beet pectin using size exclusion chromatography

Contact Info

Product

Resources

About