Patterns of ISSR and REMAP DNA markers after cryogenic preservation of spring wheat calli by dehydration method

Solov'eva, A. I.; Dolgikh, Yu. I.; Vysotskaya, O. N.; Popov, A. S.

doi:10.1134/s1021443711030162

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…Nonetheless, the potential effects of cryostorage of explants and seeds on subsequent plant growth in ex vitro conditions must be established before routine implementation in cryobanks, especially as genomic or phenotypic variation may result from tissue culture procedures commonly used in cryopreservation techniques. The dehydration phase (both physical and chemical in PVS) is the most critical for the stability of the plant material, as observed with spring wheat ( Triticum aestivum L.) and tomato ( Solanum lycopersicum L.) [ 167 ]. The duration of LN storage may also affect the parameters of plant material.…”

Section: Stability and Functional Genomics Of Ln-derived Plant Materialsmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

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Section: Stability and Functional Genomics Of Ln-derived Plant Materialsmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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“…Some of these original innovations have been patented and used for cryopreservation of plant specimens belonging to diverse taxonomical groups and material types, including seeds, cell cultures, shoot meristems, protocorms, etc. (Table 3) [123,138,[142][143][144][145][146][147][148][149][150][151][152][153][154][155][156][157][158][159][160]. IPPRAS cryobank collections are unique in terms of the diversity of specimens and represented taxa and the long-term preservation of viable plant material in LN.…”

Section: Ippras Cryobank Of Plant Genetic Resourcesmentioning

confidence: 99%

“…Some of these original innovations have been patented and used for cryopreservation of plant specimens belonging to diverse taxonomical groups and material types, including seeds, cell cultures, shoot meristems, protocorms, etc. ( Table 3 ) [ 123 , 138 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 , 156 , 157 , 158 , 159 , 160 ].…”

Section: Ippras Cryobank Of Plant Genetic Resourcesmentioning

confidence: 99%

“…Healthy seedlings were obtained for various plant species after almost 30 years of cryopreservation [ 135 ]. The identity of the recovered plant materials to source specimens was assessed by comparing their agricultural and biotechnological traits and investigating them at the genetic level [ 149 , 150 , 154 ]. Our studies indicated no significant differences in the main characteristics between plant material of various types before and after cryopreservation [ 123 , 134 , 135 , 155 , 156 ] ( Table 3 ).…”

Section: Ippras Cryobank Of Plant Genetic Resourcesmentioning

confidence: 99%

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Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

Yuorieva,

Sinetova,

Messineva

et al. 2023

Biology

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Ex situ collections of algae, cyanobacteria, and plant materials (cell cultures, hairy and adventitious root cultures, shoots, etc.) maintained in vitro or in liquid nitrogen (−196 °C, LN) are valuable sources of strains with unique ecological and biotechnological traits. Such collections play a vital role in bioresource conservation, science, and industry development but are rarely covered in publications. Here, we provide an overview of five genetic collections maintained at the Institute of Plant Physiology of the Russian Academy of Sciences (IPPRAS) since the 1950–1970s using in vitro and cryopreservation approaches. These collections represent different levels of plant organization, from individual cells (cell culture collection) to organs (hairy and adventitious root cultures, shoot apices) to in vitro plants. The total collection holdings comprise more than 430 strains of algae and cyanobacteria, over 200 potato clones, 117 cell cultures, and 50 strains of hairy and adventitious root cultures of medicinal and model plant species. The IPPRAS plant cryobank preserves in LN over 1000 specimens of in vitro cultures and seeds of wild and cultivated plants belonging to 457 species and 74 families. Several algae and plant cell culture strains have been adapted for cultivation in bioreactors from laboratory (5–20-L) to pilot (75-L) to semi-industrial (150–630-L) scale for the production of biomass with high nutritive or pharmacological value. Some of the strains with proven biological activities are currently used to produce cosmetics and food supplements. Here, we provide an overview of the current collections’ composition and major activities, their use in research, biotechnology, and commercial application. We also highlight the most interesting studies performed with collection strains and discuss strategies for the collections’ future development and exploitation in view of current trends in biotechnology and genetic resources conservation.

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“…Numerous papers report about preserva tion of genetic stability, ability of cells to divide and regenerate plants [7,8], and efficiency of the synthesis of secondary metabolites [9,10]. However, there are reports about possible modification of the genome in the course of cryopreservation; in particular, changes in DNA sequences in the samples of spring wheat calli were revealed [11]. Success of low temperature cryo conservation depends on a series of factors: genetic and morphological features of the cells, composition of conservation medium, type and concentration of cryoprotector, mode of freezing and thawing condi tions, ability of hardening and permeability to cryo protector [12].…”

Section: Introductionmentioning

confidence: 99%

Recovery of cytogenetic and physiological characteristics of a population of alfalfa cells after cryogenic storage

Волкова

Urmantseva

Бургутин

et al. 2015

Russ J Plant Physiol

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After 27 years of storage in liquid nitrogen, we managed to restore the growth of suspension cell culture of alfalfa (Medicago sativa L.). The influence of factors affecting viability of alfalfa cells in vitro was investigated together with cytogenetic and physiological characteristics after their storage at the tempera ture of liquid nitrogen (-196°C). As a result of freezing/thawing, approximately 80% of predominantly polyploid cells died, and osmotic stress was the main injurious agent. Thus, after cryogenic storage, cell culture mainly consisted of hypo and hyper haploid and hypo and hyper diploid cells. After 35 growth cycles, the restored population reached a dynamic equilibrium between the cells of different levels of ploidy with modal class (accounting for more than 50%) of polyploid cells. The strain under investigation is nota ble for a high activity of peroxidase (PO). After cryogenic storage, PO activity considerably decreased; however, during 35 growth cycles, enzyme activity gradually rose to the level characteristic of original cell culture prior to freezing. In order to protect the cells from endogenous ice nucleation, we used cryoprotec tor dimethyl sulfoxide (DMSO). It was found that DMSO was not efficient at a concentration of 7% for cell protection against osmotic stress and exerted a moderate antiradical influence in respect to superoxide anion but improved viability of recovered cells and affected activity of PO and aldehyde reductase and the level of lipid peroxidation.

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Patterns of ISSR and REMAP DNA markers after cryogenic preservation of spring wheat calli by dehydration method

Cited by 9 publications

References 14 publications

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

Recovery of cytogenetic and physiological characteristics of a population of alfalfa cells after cryogenic storage

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