Patterns of invasion and routes of tumor entry into the mandible by oral squamous cell carcinoma

Brown, James S.; Lowe, Derek; Kalavrezos, Nicholas; D'Souza, Jacob; Magennis, Patrick; Woolgar, Julia A.

doi:10.1002/hed.10062

Cited by 173 publications

(113 citation statements)

References 17 publications

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“…We next examined the protein levels of OPG in culture media from POBs treated with or without 1,25D 3 and cocultures of POBs with human oral SCC cells using mouse OPG EIA system. Measurements were made every 2 days and the mouse OPG protein levels in the culture media reached a maximum at day 4.…”

Section: Suppression Of Opg Expression By Pobs Is Involved In Oral Scmentioning

confidence: 99%

“…3,4 Previous studies have suggested that the bone destruction associated with carcinoma invasion is mediated by osteoclasts rather than directly by the carcinoma. 3,4 Invasion of the mandible by oral SCC is currently established only by radiological inspection. The cellular and molecular mechanisms regulating bone invasion and osteoclastic bone resorption by oral SCC are poorly understood.…”

mentioning

confidence: 99%

“…[5][6][7] Osteoclastic bone resorption consists of multiple steps: proliferation of osteoclast progenitors, differentiation of progenitors into mononuclear prefusion osteoclasts (pOCs), fusion of pOCs into multinucleated osteoclasts, activation of osteoclasts to resorb bone and apoptosis. [5][6][7][8] Multinucleated cells (MNCs) with osteoclast characteristics, including tartrate-resistant acid phophatase (TRAP) activity, calcitonin receptors and pit-forming activity on dentin slices, can be found in cocultures of mouse bone marrow cells (BMCs) and osteoblasts/stromal cells in the presence of osteotropic factors such as 1a,25-dihydroxyvitamine D 3 (1,25D 3 ), parathyroid hormone (PTH) and interleukin-11 (IL-11). We have previously shown that several cytokines and hormones regulate the differentiation and function of osteoclasts.…”

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confidence: 99%

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Oral squamous cell carcinoma cells induce osteoclast differentiation by suppression of osteoprotegerin expression in osteoblasts

Tada

Jimi

Okamoto

et al. 2005

Intl Journal of Cancer

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The invasion of oral squamous cell carcinoma (SCC) cells into the mandibular bone is a common clinical problem. It has been reported that BHY cells, a human oral SCC cell line, are capable of invading mandibular bone of nude mice. These results led us to examine possible mechanisms of osteoclastogenesis induced by BHY cells using in vitro culture systems. When BHY cells were cocultured with mouse bone marrow cells (BMCs), only few osteoclasts were formed, even though BHY cells express the receptor activator of NF-jB ligand (RANKL). However, adding BHY cells to a coculture of mouse primary osteoblasts (POBs) and BMCs markedly induced osteoclastogenesis in the absence of osteotropic factors. Furthermore, another oral SCC cell line, HSC-2, which does not express RANKL, also induced osteoclastogenesis in our cocultures. These effects were significantly, but not completely, inhibited by adding osteoprotegerin (OPG). In addition, we also found that TNFa released from these cells partially contributes to osteoclastogenesis via a RANKL-independent mechanism. Adding BHY or HSC-2 cells suppressed mouse OPG mRNA expression and protein production by POBs in cocultures of POBs and human oral SCC cells. This finding is consistent with the result that BHY cells and HSC-2 cells did not enhance osteoclastogenesis in cocultures of BMCs and POBs from OPG-deficient mice. Immunohistochemical analysis showed a reduction of OPG expression in osteolytic lesions as compared to normal lesions from oral SCC patients. Therefore, oral SCC-induced suppression of OPG expression in POBs appears critical for osteoclastogenesis, rather than expression of RANKL in SCC cells. ' 2005 Wiley-Liss, Inc.Key words: squamous cell carcinoma; RANKL; OPG; osteoclastogenesis Oral cancer most commonly involves the tissue of the tongue and the lips, and it can also occur on the floor of the mouth, gingiva or plate.1,2 The vast majority of oral cancers are squamous cell carcinomas (SCCs) 1,2 and oral SCCs frequently invade the mandibular bone. Advanced oral SCC has a high mortality and treatment is complicated by the disruption of speech and swallowing after surgical resection. It is generally agreed that patients with mandibular invasion should be treated surgically, but the extent of mandibular resection required remains controversial. Resection of the mandibular bone leads to physical damage as well as frequent psychological problems for the patients.It is well known that oral SCC invades the mandibular bone by direct extension and not by metastasis.3,4 Previous studies have suggested that the bone destruction associated with carcinoma invasion is mediated by osteoclasts rather than directly by the carcinoma.3,4 Invasion of the mandible by oral SCC is currently established only by radiological inspection. The cellular and molecular mechanisms regulating bone invasion and osteoclastic bone resorption by oral SCC are poorly understood.Osteoclasts are multinucleated cells that are responsible for bone resorption. [5][6][7] Osteoclastic bone resorption consists...

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Section: Suppression Of Opg Expression By Pobs Is Involved In Oral Scmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Oral squamous cell carcinoma cells induce osteoclast differentiation by suppression of osteoprotegerin expression in osteoblasts

Tada

Jimi

Okamoto

et al. 2005

Intl Journal of Cancer

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“…In situations where the tumor is either invading towards or involving the skin, resection of 1 to 2 cm of normal skin abutting the tumor is required [18]. In tumors reaching or invading the mandible or maxillary alveolus, a strategy similar to that of tongue is to be adopted in resecting the bone as a margin [19].…”

Section: Buccal Mucosamentioning

confidence: 99%

Site-wise Differences in Adequacy of the Surgical resection Margins in Head and Neck Cancers

Vidhyadharan

Augustine

Kudpaje

et al. 2014

Indian J Surg Oncol

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Adequacy of surgical resection decided by the margin status is important in attaining a good local control and better survival in Head and neck Cancers. Conventionally, a measured distance between the tumor edge and the cut edge of the specimen is taken as the margin. A margin more than 5 millimeter (mm) is considered clear, less than 5 mm is close and less than one mm is denoted as involved. The concept of this adequacy varies between the different sites and subsites in head and neck. The purpose of this paper is to review the current evidence that describes the adequacy of surgical margin status and their variability among the sites and sub-sites in the head and neck.

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“…Most of the times it involves the mandible either by direct invasion of tumor, or through the alveolar ridge into the canal of inferior alveolar nerve [1].…”

Section: Introductionmentioning

confidence: 99%

Primary Intraosseous Carcinoma with Nodal Metastasis Masquerading as Ameloblastom—A Rare Case Report

Sharma

Shah²,

Patel

et al. 2011

Indian J Surg Oncol

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Patterns of invasion and routes of tumor entry into the mandible by oral squamous cell carcinoma

Cited by 173 publications

References 17 publications

Oral squamous cell carcinoma cells induce osteoclast differentiation by suppression of osteoprotegerin expression in osteoblasts

Oral squamous cell carcinoma cells induce osteoclast differentiation by suppression of osteoprotegerin expression in osteoblasts

Site-wise Differences in Adequacy of the Surgical resection Margins in Head and Neck Cancers

Primary Intraosseous Carcinoma with Nodal Metastasis Masquerading as Ameloblastom—A Rare Case Report

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