Patterns of Genetic Variability in Colonized Strains of Lutzomyia longipalpis (Diptera: Psychodidae) and Its Consequences

Mukhopadhyay, J.; Rangel, Elizabeth Ferreira; Ghosh, Kashinath; Munstermann, Leonard E.

doi:10.4269/ajtmh.1997.57.216

Cited by 48 publications

(54 citation statements)

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“…Equally interesting from the data compiled in this table is that it is only within Brazil where broadly separated Lu. longipalpis populations have been considered either a single species (Mukhopadhyay et al 1997, 1998a,b, Mutebi et al 1999, Azevedo et al 2000, Arrivillaga et al 2003, de Queiroz Balbino et al 2006 or a species complex (Ward et al 1983, 1988, Yin et al 1999, Souza et al 2002, Bauzer et al 2002a,b, Maingon et al 2003, Bottecchia et al 2004, Hamilton et al 2004, Watts et al 2005) depending on the markers used. Therefore the controversy concerning the existence of a complex is centred on data from Brazilian populations.…”

Section: Lu Longipalpis a Species Complex?mentioning

confidence: 99%

Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review

Bauzer

Souza

Maingón

et al. 2007

Mem. Inst. Oswaldo Cruz

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Section: Lu Longipalpis a Species Complex?mentioning

confidence: 99%

Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review

Bauzer

Souza

Maingón

et al. 2007

Mem. Inst. Oswaldo Cruz

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“…Mukhopadhyay et al (1997) compared the genetic profiles of 14 loci in five colonies of Lu. longipalpis from different geographical regions with those from several field populations in Brazil.…”

Section: Isozymic Variabilitymentioning

confidence: 99%

The status of the Lutzomyia longipalpis species complex and possible implications for Leishmania transmission

Uribe

1999

Mem. Inst. Oswaldo Cruz

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“…19 It has been reported that laboratory colonization of sand flies reduces natural genetic variability, and might foster selection for certain traits that are normally suppressed in field populations. [20][21][22] The loss of genetic variation as a result of colonization might figure prominently in the protection observed in mice preimmunized with SGH of long-term colonized P. papatasi and challenged with L. major co-inoculated with the same type of SGH. 5 Antigenic variation of salivary gland proteins of field populations of P. papatasi similar to that observed in Lu.…”

mentioning

confidence: 99%

Lack of Protection of Pre-Immunization with Saliva of Long-Term Colonized Phlebotomus papatasi against Experimental Challenge with Leishmania major and Saliva of Wild-Caught P. papatasi

Ahmed¹,

Kaabi²,

Chelbi³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Several studies have shown that pre-immunization of mice with needle-injected saliva or pre-exposure to uninfected bites of Phlebotomus papatasi provided protection against infection with Leishmania major , the etiologic agent of zoonotic cutaneous leishmaniasis (ZCL).1-3 These studies were performed by using long-term laboratory colonized sand flies mainly because of the difficulty of working with wild-caught flies. A puzzling fact is that people in leishmaniasis-endemic areas succumb of ZCL despite the high frequency of uninfected bites compared with infected ones. 4 We showed in a previous study that pre-immunization of mice with salivary gland homogenate (SGH) of wild-caught P. papatasi does not confer protection against L. major co-inoculated with the same type of SGH compared with a significant protection obtained when pre-immunization and challenge were performed with SGH of long-term colonized flies. 5 It was reported that colonized and wild-caught Lutzomyia longipalpis differ in the composition and the amount of salivary proteins and these differences may account for the lower effect observed on the modulation of experimental Leishmania infection by wild-caught SGH. 6,7 Thus, these studies provide good evidence that the outcome of Leishmania infection differs significantly between colonized and wild-caught salivary gland proteins. Our aim in this study was to assess the protective effect of pre-immunization with SGH of long-term colonized P. papatasi on experimental L. major challenge co-inoculated with SGH of wild-caught P. papatasi .Phlebotomus papatasi (Tunisian strain that originated in the Governorate of Sidi Bouzid) have been reared at the Vector Ecology Laboratory of the Institut Pasteur de Tunis since 2003. 8 This colony is maintained without being supplemented periodically with wild-caught P. papatasi . Generation F 39 was used in this study. Wild sand flies were collected using CDC light traps from animal shelters located in the village of Felta (Governorate of Sidi Bouzid), a focus highly endemic for ZCL.9 Preparation of SGH from laboratory-reared and wild-caught P. papatasi was performed as described. 5 A highly virulent strain of L. major , MHOM/TN/95/GLC94, isolated from a patient in Tunisia was used in this study. 5 We used female BALB/c mice (6-8 weeks of age) bred in the animal facility of the Institut Pasteur de Tunis.Mice were immunized intradermally in the right ear with the equivalent of two pairs of salivary glands in 10 µL of phosphate-buffered saline (PBS). Two groups of 10 mice each were pre-immunized with SGH of long-term colonized female P. papatasi (F 39 ), once a week for two weeks. In the fourth week, the first [CSGH (F 39 )-L.m+CSGH (F 39 )] and the second [CSGH (F 39 )-L.m+WSGH] groups were challenged with 10 6 stationary phase L. major promastigotes in 50 µL of PBS co-inoculated subcutaneously in the right hind footpads with SGH of long-term, colonized and SGH of wild-caught, female P. papatasi , respectively. Three control groups of 10 mice each were used in this stud...

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Patterns of Genetic Variability in Colonized Strains of Lutzomyia longipalpis (Diptera: Psychodidae) and Its Consequences

Cited by 48 publications

References 0 publications

Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review

Lutzomyia longipalpis in Brazil: a complex or a single species? A mini-review

The status of the Lutzomyia longipalpis species complex and possible implications for Leishmania transmission

Lack of Protection of Pre-Immunization with Saliva of Long-Term Colonized Phlebotomus papatasi against Experimental Challenge with Leishmania major and Saliva of Wild-Caught P. papatasi

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