Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas

Dockhorn‐Dworniczak, Barbara; Franke, Werner W.; Schröder, S.; Czernobilsky, Bernard; Gould, Victor E.; Böcker, W.

doi:10.1111/j.1432-0436.1987.tb00151.x

Cited by 51 publications

(35 citation statements)

References 94 publications

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“…Confirming previous reports on the CK pattern of the normal thyroid gland [3,6,11,16,23,24,27,28,31], we observed the constant and high expression of simple epithelial-type CKs 7 and 18 and, to a lesser degree, of CKs 8 and 19. There was absence of expression of keratins characteristic of stratified epithelia, such as CKs 1, 4, 5/6 and 13, and also CK 20, in normal thyroid tissues.…”

Section: Discussionsupporting

confidence: 94%

“…The low-molecular-weight CK 19 is a major component of simple epithelia and a minor component of stratified epithelia, where it is expressed only in the basal cell layer. We have confirmed previous observations [6,23,27] showing that CK 19 is weakly and focally expressed in the normal follicular epithelium of the thyroid gland.…”

Section: Discussionsupporting

confidence: 93%

“…Several reports on the cytoskeletal components of follicular cells in the thyroid gland, either focusing on the CK pattern only [23,24,27] or including the detection of other cytoplasmic filaments [3,6,11,16,28,31], have been published to date. The results of these studies are contradictory as far as the types of CKs expressed by thyroid tumours are concerned.…”

Section: Introductionmentioning

confidence: 99%

“…The results of these studies are contradictory as far as the types of CKs expressed by thyroid tumours are concerned. The presence or absence of stratified epithelial-type CKs in papillary carcinomas has not been established definitively [3,6,11,16]. The usefulness of a particular low-molecular-weight CK (CK 19) in the differential diagnosis between papillary and follicular carcinoma remains controversial [6, 23, 27, …”

Section: Introductionmentioning

confidence: 99%

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Pattern of expression of intermediate cytokeratin filaments in the thyroid gland: an immunohistochemical study of simple and stratified epithelial-type cytokeratins

et al. 1997

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The expression of simple and stratified epithelial-type cytokeratin (CK) intermediate filaments was evaluated by immunohistochemistry in a series of 41 papillary carcinomas, 10 follicular carcinomas, 2 poorly differentiated carcinomas and 34 specimens of normal thyroid parenchyma and lymphocytic thyroiditis. The aim of the study was to establish the CK profile of normal thyroid and thyroid carcinomas in order to clarify the putative application of CK immunostaining in diagnostic surgical pathology, and to evaluate whether the process of neoplastic transformation and tumour progression in the thyroid may be associated with any particular change in CK expression. Normal thyroid strongly expressed simple epithelial-type CKs 7 and 18 and, to a lesser degree, CKs 8 and 19, but did not express stratified epithelial-type CKs. The same pattern was found in lymphocytic thyroiditis, though the CK 19 immunoreactivity was stronger in these lesions than in the normal thyroid. Papillary and follicular thyroid carcinomas shared the expression of simple epithelial-type CKs 7, 8, 18 and 19. Immunoreactivity for CK 19 was frequently stronger and more widely distributed within each particular tumour in papillary than in follicular carcinomas, but it could also be detected, at least focally, in every follicular carcinoma. Strong expression of CK 19 highlighted small foci of papillary carcinoma not easily identifiable by conventional histological examination. Stratified epithelial-type CKs 5/6 and 13 were detected in a high percentage of papillary carcinomas, in contrast to their absence in follicular carcinomas and normal thyroid. The CK pattern was similar in primary and metastatic papillary carcinomas. We conclude that papillary carcinoma of the thyroid presents a distinct CK profile that may be used for diagnostic purposes.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pattern of expression of intermediate cytokeratin filaments in the thyroid gland: an immunohistochemical study of simple and stratified epithelial-type cytokeratins

et al. 1997

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“…These differences are probably not only caused by antigen masking but most probably they reflect true differences in synthesis, as shown for other simple epi thelial keratins [39]. Heterogeneous distribution of this polypeptide has been described in some pathological conditions, indicating that its expression is highly vari able between different tumors of the same cytomorphologic type and also between cells of an individual tumor [40][41][42]. In vitro, probably due to its exceptional gene organization, cytokeratin No.…”

Section: Discussionmentioning

confidence: 99%

Expression of Cytokeratin No. 19 Polypeptide in Genital Papillomavirus Lesions

Leoncini¹,

Petracca²,

Ruggiero³

et al. 1990

Gynecol Obstet Invest

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A series of 23 punch biopsies proved to contain human papillomavirus (HPV) type 16 and with established clinical course (including HPV-NCIN, HPV-CIN I, and HPV-CIN II lesion), and 18 additional biopsies of HPV 6-, 11-, 16- or 18-induced genital lesions were analyzed immunohistochemically for expression of cytokeratin No. 19 polypeptide. An immunoperoxidase-ABC technique was used with a polyclonal antibody raised against a synthetic nonapeptide corresponding to the residues 2–10 of the NH₂-end, non-alpha-helical region. This polyclonal cytokeratin No. 19 antibody stained mainly (but not exclusively) the basal cells of the normal exocervical epithelium (heterogeneous pattern). Basal cell staining was intense slightly more frequently in HPV-CIN than HPV-NCIN lesions, i.e., ++ or more in 14/24 (58.3%) versus 8/17 (47.0%), respectively. The difference was more marked in the staining of the superficial cells, 70.8 and 58.8% showing intense expression of cytokeratin No. 19, respectively. In 6 (21.4 %) of the 28 HPV 16 lesions, basal cell layer was intensely stained, as contrasted to none of the 13 HPV 6, 11 or 18 lesions. The most distinct feature was the well-defined granular staining pattern of the superficial layer in 8 out of 10 HPV 6/11 lesions, as contrasted to the homogeneous pattern in 24 out of 28 HPV-16-infected lesions. In superficial cells, regressed lesions exhibited intense staining in 9/13 (69.2%), as compared with only 4/10 (40%) of the progressed lesions. These deviations from the normal cytokeratin No. 19 expression pattern in cervical squamous epithelium might be associated with progression of the lesions from HPV-NCIN (usually of HPV 6/11 origin) into higher grades of HPV-CIN (usually caused by HPV 16).

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Neoexpression of the epithelial adhesion complex antigens in thyroid tumours is associated with proliferation and squamous differentiation markers

et al. 1998

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Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas

Cited by 51 publications

References 94 publications

Pattern of expression of intermediate cytokeratin filaments in the thyroid gland: an immunohistochemical study of simple and stratified epithelial-type cytokeratins

Pattern of expression of intermediate cytokeratin filaments in the thyroid gland: an immunohistochemical study of simple and stratified epithelial-type cytokeratins

Expression of Cytokeratin No. 19 Polypeptide in Genital Papillomavirus Lesions

Neoexpression of the epithelial adhesion complex antigens in thyroid tumours is associated with proliferation and squamous differentiation markers

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