Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodes

Saleem, Saira; Jamshed, Arif; Faisal, Saima; Hussain, Raza; Tahseen, Muhammad; Loya, Asif; Sutton, Chris W.

doi:10.1186/s12935-014-0143-3

Cited by 11 publications

(10 citation statements)

References 23 publications

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“…For example, Saleem et al found that lymph node metastatic OSCC express higher levels of CD44/CD24. 49 It has been reported that downregulation of CD24 inhibits proliferation while inducing apoptosis in malignant cells. 50 Further, CD24 may be a potential marker for cancer stem cells.…”

Section: Discussionmentioning

confidence: 99%

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

et al. 2016

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CD24 expression has been implicated in the oncogenesis of multiple types of cancer and high tumor expression is considered a poor prognosis factor; however, the role of CD24 in oral cancer progression is unknown. Unlike other cancer types, we found that higher CD24 levels in human oral cancers are correlated to lower clinical stage and better overall survival. We then dissected the role of CD24 and mechanisms in oral cancer pathogenesis in mice using a genetic strategy and demonstrated that CD24 deficiency increased the oral cavity tumor burden in response to the carcinogen 4-nitroquioline 1-oxide (4-NQO). Immune profile analysis showed a significant expansion as well as increased suppressive function of myeloid-derived suppressor cells (MDSCs) in CD24 ¡/¡ mice, but no apparent impairment in T cells, B cells, or dendritic cells. Further, studies with an orthotopically transplanted syngeneic squamous carcinoma model in the tongue of CD24 ¡/¡ and CD24 C/¡ mice confirmed the protective roles of CD24 against cancer. Moreover, the difference in tumor growth between CD24 ¡/¡ and CD24 C/¡ mice was blunted by immunodepletion of MDSCs. We conclude that CD24 expression impedes MDSC expansion and function, and thus slows oral cancer oncogenesis. This study is the first to examine the role of CD24 in a de novo oral cancer model, and it highlights the need to consider the immune regulatory roles of CD24 in the development of CD24-targeted therapy for cancer.

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Section: Discussionmentioning

confidence: 99%

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

et al. 2016

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“…As such, tumor spheres extracted from OSCC cells show higher stem-like features. They show greater volume of expression of pluripotent transcription agents such as Lin28, Nanog, KLF4, Oct4, and Sox2 in comparison to the respective adherent mono-layer cells [26,60,70,71]. Moreover, influential expression of CSC specific markers, including CD44 and ALDH1 is expressed by oral tumor sphere-forming cells [60,61,72].…”

Section: Oral Cancer Stem Cells Isolationmentioning

confidence: 99%

Cancer stem cells and oral cancer: insights into molecular mechanisms and therapeutic approaches

2020

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Cancer stem cells (CSCs) have been identified as a little population of cancer cells, which have features as the same as the cells normal stem cells. There is enough knowledge of the CSCs responsibility for metastasis, medicine resistance, and cancer outbreak. Therefore, CSCs control possibly provides an efficient treatment intervention inhibiting tumor growth and invasion. In spite of the significance of targeting CSCs in treating cancer, few study comprehensively explored the nature of oral CSCs. It has been showed that oral CSCs are able to contribute to oral cancer progression though activation/inhibition a sequences of cellular and molecular pathways (microRNA network, histone modifications and calcium regulation). Hence, more understanding about the properties of oral cancers and their behaviors will help us to develop new therapeutic platforms. Head and neck CSCs remain a viable and intriguing option for targeted therapy. Multiple investigations suggested the major contribution of the CSCs to the metastasis, tumorigenesis, and resistance to the new therapeutic regimes. Therefore, experts in the field are examining the encouraging targeted therapeutic choices. In spite of the advancements, there are not enough information in this area and thus a magic bullet for targeting and eliminating the CSCs deviated us. Hence, additional investigations on the combined therapies against the head and neck CSCs could offer considerable achievements. The present research is a review of the recent information on oral CSCs, and focused on current advancements in new signaling pathways contributed to their stemness regulation. Moreover, we highlighted various therapeutic approaches against oral CSCs.

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“…Interestingly, CD44+ cells from hyperplastic or non-cancer tongue tissue did not produce spheres. Besides, primary cultures from metastatic TSCC specimens showed stronger CD44/CD24 expression and produced much more spheres in significantly shorter duration compared to those of node negative TSCC and normal tongue specimens (Saleem et al 2014).…”

Section: Cd44mentioning

confidence: 89%

“…It has been shown to be deregulated in several cancers including TSCC (Lindquist et al 2012, Yanamoto et al 2014. CD44 has important roles during carcinogenesis and metastasis in TSCC and is involved in several carcinogenic processes like proliferation, migration, cellcell interactions, and apoptosis in TSCC cells (Saleem et al 2014. Its expression shows significant correlation with several other critical biomarkers such as CD24 ( The expression of CD44 in TSCC samples was initially investigated in 1996, where 83 head and neck squamous cell carcinoma (including 12 TSCC specimens) samples were used for immunohistochemical analysis of CD44 variants (v5, v6, v7, v7-v8, and v10) in normal, dysplastic squamous epithelia, primary and metastatic squamous cell carcinoma samples.…”

Section: Cd44mentioning

confidence: 99%

Current Cancer Stem Cell Biomarkers in Tongue Squamous Cell Carcinoma

Karataş¹,

Teber²,

Yılmaz³

et al. 2018

Trakya University Journal of Natural Sciences

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Tongue squamous cell carcinoma (TSCC) is known to be the most malignant cancer type amongst other oral cancers with increasing incidence and mortality rates in the past five years. Since the life expectancy for TSCC patients is limited and the current chemo-radiotherapy treatments are not curative, novel biomarkers are urgently needed. As many other solid tumors, TSCC has a heterogeneous cancer cell population, which includes a small subpopulation identified as cancer stem cells (CSCs) that are considered as the driving force for tumor initiation, development, spread, recurrence, and resistance to chemoradiotherapy. Although the underlying molecular mechanisms of how CSCs are involved in the carcinogenesis are not completely understood, scientists and clinicians aim to utilize those cells as therapeutic tools in fight against different cancer types including TSCC. Here, we reviewed and summarized important findings and the most current literature to shed light on the potential of cancer stem cells markers in TSCC. Possible functions of CSCs biomarkers in TSCC pathogenesis during cancer initiation, progression, invasion or metastasis are also summarized.

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Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodes

Cited by 11 publications

References 23 publications

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

Cancer stem cells and oral cancer: insights into molecular mechanisms and therapeutic approaches

Current Cancer Stem Cell Biomarkers in Tongue Squamous Cell Carcinoma

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