Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells

Nataro, James P.; Kaper, James B.; Robins‐Browne, Roy M.; Prado, Valeria; Víal, Pablo A.; Levine, Myron M.

doi:10.1097/00006454-198709000-00008

Cited by 596 publications

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“…Thus, diffusely adherent E. coli (DAEC) are bacteria that are dispersed over the surface of the cell [12] and possess none of the virulence factors known to be characteristic of other diarrhoeagenic E. coli. DAEC seems to be a heterogeneous group of strains composed probably of true pathogens that cause infantile diarrhoea (especially among children aged 2-5 years) and non-pathogenic bacteria [13].…”

Section: Introductionmentioning

confidence: 99%

“…DAEC seems to be a heterogeneous group of strains composed probably of true pathogens that cause infantile diarrhoea (especially among children aged 2-5 years) and non-pathogenic bacteria [13]. Enteroaggregative E. coli (EAEC) adhere to tissue culture cells and coverslips in a characteristic stacked bricklike configuration [12]. Many EAEC strains possess a 60-MDa aggregative adherence plasmid (pAA) [14].…”

Section: Introductionmentioning

confidence: 99%

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Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea

Paciorek

2002

Journal of Medical Microbiology

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Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enterohaemolysin, the genes encoding intimin (eae), bundle-forming pili (bfp), Shiga toxins I and II (stxI, stxII ) and cytotoxic necrotising factor types 1 and 2 (cnf 1, cnf 2) was investigated by PCR. Adhesion to HEp-2 cell monolayers was also tested and selected strains were investigated for the presence of P-fimbriae and haemolytic activity. Typical enteropathogenic E. coli isolates (typical EPEC, strains possessing eae and bfp, but not stx) were not found. The particular classes of E. coli among 427 isolates from ill children were: atypical EPEC (eae bfp, stx 2 ), 21.3%; Shiga toxin-producing E. coli (STEC), 0.7%; diffusely adherent E. coli (DAEC), 4%; enteroaggregative E. coli (EAEC), 16.9%; necrotoxic E. coli type 1 (NTEC1), 0.2%; and cell-detaching E. coli (CDEC), 29%. With the exception of STEC, all the above classes of E. coli were found among the isolates from healthy children which comprised: atypical EPEC 8.0%, DAEC 6.7%, EAEC 17.3%, NTEC1 14.0% and CDEC 40.0%. Cell detachment (CD) was significantly associated with 3-h haemolytic activity. There was also strong correlation between haemolytic activity (Hly) and the presence of P-fimbriae. No correlation was found between the presence of the cnf1 gene and CD, Hly or P-fimbriae.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea

Paciorek

2002

Journal of Medical Microbiology

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“…These include: enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), verotoxinproducing E. coli (VTEC) some strains of which are enterohaemorrhagic (EHEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAggEC) and diffusely adherent E. coli (DAEC). EAggEC is associated with persistent diarrhoea in children [2] and it has been identified by a tissue culture adherence assay, where it presents an aggregative pattern, characterised by the fact that bacteria adhere to each other and appear as stacked-brick clumps on the HEp-2 cell [3]. Although EAggEC has been recognised as an important cause of acute and persistent diarrhoea in children in developing countries [2,41, few studies have sought the presence of EAggEC in children in developed countries [ 5 , 61.…”

Section: Introductionmentioning

confidence: 99%

A case-control study of diarrhoea in children caused by Escherichia coli producing heat-stable enterotoxin (EAST-1)

Vila

Vargas²,

Gascón³

et al. 1998

Journal of Medical Microbiology

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Escherichia coli strains associated with diarrhoeal disease have been classified into several types according to the pathogenic mechanism. Among these, enteroaggregative E. coli strains (EAggEC) have been associated with persistent childhood diarrhoea. Some strains of EAggEC produce a heat-stable toxin (EAST-1) that differs from others described previously. The main goal of this case-control study was to determine the prevalence of EAggEC and EAST-l-producing E. coli strains as a cause of diarrhoea in children in Spain and to study their in-vitro susceptibility to 21 antimicrobial agents. In the case group (115 children) 22 (190/,) isolates and four (3.5%) isolates were EAST-1-producing E. coli and EAggEC, respectively, whereas in the control group (79 children) four (5%) isolates produced EAST-1 (p = 0.005) and three (3.8%) isolates were EAggEC. The present study suggests that EAST-l-producing E. coli strains are associated with diarrhoeal diseases in Spanish children, whereas EAggEC strains are not. Moreover, EAST-l-producing E. coli strains showed a high susceptibility to all the antimicrobial agents tested except for ampicillin.

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“…The term enteroaggregative is derived from the pattern of adherence of bacteria to HEp-2 cells in culture. 6 EAggEC strains are a major cause of persistent diarrhea in children in the developing world. The aggregative adherence phenotype is associated with a bundle-forming pilus referred to as AAF/1.…”

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confidence: 99%

Prevalence of the Enteroaggregative Escherichia Coli Heat-Stable Enterotoxin 1 Gene and Its Relationship with Fimbrial and Enterotoxin Genes in E. Coli Isolated from Diarrheic Piglets

2001

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Abstract.A total of 720 Escherichia coli strains isolated from diarrheic piglets on 756 swine farms were screened for the presence of the enteroaggregative E. coli heat-stable enterotoxin 1 (EAST1) gene by polymerase chain reaction (PCR). Escherichia coli strains that carried EAST1 genes were also tested by PCR for the presence of 4 fimbriae (F4, F5, F6, F41), 2 heat-stable enterotoxins (STa and STb), and 1 heat-labile enterotoxin (LT) gene. One hundred sixty-four (22.7%) of the 720 E. coli isolates carried genes for EAST1. Of these 164 isolates, 62 (37.8%) carried EAST1 genes only, 11 (6.7%) carried genes for at least 1 of the fimbrial adhesins, 51 (31.1%) carried genes for at least 1 of the enterotoxins, and 40 (23.8%) carried genes for at least 1 of the fimbrial adhesins and enterotoxins. Forty-six percent of strains that carried EAST1 genes carried STa genes, and 16% of strains that carried EAST1 genes carried F4. The isolation rate of enterotoxigenic E. coli strains carrying genes for EAST1 gene was 63%. The 6 major genotypes observed in this study (in decreasing order) were EAST1ϩ, EAST1ϩSTaϩ, EAST1ϩSTaϩSTbϩ, EAST1ϩSTaϩF5ϩ, EAST1ϩSTaϩF4ϩ, and EAST1ϩSTbϩF4ϩ. EAST1 is widely prevalent among diarrheagenic strains of E. coli and may represent an important virulence determinant in the pathogenesis of enteric colibacillosis of preweaned pigs.Escherichia coli is a major cause of diarrhea in human beings and animals. The 4 major groups are enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), and enterohemorrhagic E. coli (EHEC). 5 Recently, evidence has accumulated to implicate a fifth distinct diarrheagenic category, enteroaggregative E. coli (EAggEC). The term enteroaggregative is derived from the pattern of adherence of bacteria to HEp-2 cells in culture. 6 EAggEC strains are a major cause of persistent diarrhea in children in the developing world. The aggregative adherence phenotype is associated with a bundle-forming pilus referred to as AAF/1. The genes for AAF/1 and a heat-stable enterotoxin 1 (EAST1) are carried on a 60-MD plasmid. EAST1 is genetically and immunologically distinct from heat-stable enterotoxin a (STa) produced by ETEC strains. 14,15 In addition to EAggEC, the EAST1 gene is also carried by human, porcine, and bovine ETEC strains. 1,10,15 The EAST1 gene sequence of F4ϩ ETEC strains is slightly different from that of human-colonizing ETEC strains. [14][15][16] The gene encoding EAST1 is highly prevalent among other groups of diarrheagenic E. coli isolated from humans, e.g., EHEC, ETEC, and EAggEC. 10 Although enteric colibacillosis is common in piglets, little is known concerning the prevalence of EAST1-positive E. coli and, thus, its potential significance in isolates from this species. The purpose of this study was to investigate the prevalence of the EAST1 gene in E. coli isolated from diarrheic piglets and to determine its relationship with other fimbrial (F4, F5, F6, and F41) and enterotoxin (STa, STb, and LT) genes by using polymerase chain reac...

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Patterns of adherence of diarrheagenic Escherichia coli to HEp-2 cells

Cited by 596 publications

References 0 publications

Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea

Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea

A case-control study of diarrhoea in children caused by Escherichia coli producing heat-stable enterotoxin (EAST-1)

Prevalence of the Enteroaggregative Escherichia Coli Heat-Stable Enterotoxin 1 Gene and Its Relationship with Fimbrial and Enterotoxin Genes in E. Coli Isolated from Diarrheic Piglets

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