Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter

Abstract

Patterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter. © 2016 Elizabeth V. Koudan, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 45 RESEARCH ARTICLEPatterning of tissue spheroids biofabricated from human fibroblasts on the surface of electrospun polyurethane matrix using 3D bioprinter Abstract: Organ printing is a computer-aided additive biofabrication of functional three-dimensional human tissue and organ constructs according to digital model using the tissue spheroids as building blocks. The fundamental biological principle of organ printing technology is a phenomenon of tissue fusion. Closely placed tissue spheroids undergo tissue fusion driven by surface tension forces. In order to ensure tissue fusion in the course of post-printing, tissue spheroids must be placed and maintained close to each other. We report here that tissue spheroids biofabricated from primary human fibroblasts could be placed and maintained on the surface of biocompatible electrospun polyurethane matrix using 3D bioprinter according to desirable pattern. The patterned tissue spheroids attach to polyurethane matrix during several hours and became completely spread during several days. Tissue constructions biofabricated by spreading of patterned tissue spheroids on the biocompatible electrospun polyurethane matrix is a novel technological platform for 3D bioprinting of human tissue and organs.

Multiparametric Analysis of Tissue Spheroids Fabricated from Different Types of Cells

“…When placed on an adhesive substrate, cell aggregates can quickly disassemble producing an even tissue construct with desirable thickness. [ 49,53 ]…”

Advances in Hybrid Fabrication toward Hierarchical Tissue Constructs

“…(D) reproduced under the terms and conditions of the Creative Commons CC BY 4.0 License. [ 30 ] Copyright 2016, The Authors, Published by Whioce Publishing Pte Ltd. (E) reproduced under the terms and conditions of the Creative Commons CC BY 4.0 License. [ 46 ] Copyright 2016, The Authors, Published by PLOS ONE.…”

Scalable biofabrication and morphology evaluation of tissue spheroids

“…В качестве адгезивного материала могут использоваться пластиковое покрытие чашек Петри, многолуночных планшетов, биосовместимый матрикс, полученный методом электроспиннинга, и другие субстраты. Было показано, что тканевые сфероиды, полученные из первичных фибробластов человека, прикрепляются к адгезивному матриксу из биосовместимого полиуретана за 4 часа и полностью распластываются на нем в течение 7 дней, а сфероиды из клеток H-1975 (клеточная линия аденокарциномы легкого) -в течение 36 часов [31,32]. Оценка кинетики распластывания тканевых сфероидов позволяет охарактеризовать их способность восстанавливать структуру поврежденной ткани, что особенно важно для замещения кожных дефектов.…”