Patterning multiple cell types in co-cultures: A review

Goubko, Mikhail; Cao, Xudong

doi:10.1016/j.msec.2009.02.016

Cited by 88 publications

(64 citation statements)

References 90 publications

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“…[1][2][3][4][5][6] Geometric features of cells and cell aggregates play important roles in regulating various cell behaviors, including cell growth, 1 differentiation, 1,5,7 polarity, 8,9 and migration. 10,11 Despite its biological importance, multiple cell type coculture patterning systems are not as commonly used at least in part because of the often tedious device fabrication and cell patterning steps required.…”

mentioning

confidence: 99%

Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures

Torisawa

Mosadegh

Cavnar

et al. 2011

Tissue Engineering Part C: Methods

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This article describes a simple and rapid cell patterning method to form co-culture microarrays in commercially available Transwells. A thin poly(dimethylsiloxane) (PDMS) layer is printed on the underside of a Transwell using a PDMS stamp. Arbitrary cellular patterns are generated according to the geometric features of the thin PDMS layer through hydrodynamic forces that guide cells onto the membrane only over the PDMS-uncoated regions. Micropatterns of surface-adhered cells (we refer to this as two-dimensional) or non-surface-adhered clusters of cells (we refer to this as three-dimensional) can be generated depending on the surface treatment of the filter membrane. Additionally, co-cultures can be established by introducing different types of cells on the membrane or in the bottom chamber of the Transwell. We show that this co-culture method can evaluate mouse embryonic stem (mES) cell differentiation based on heterogeneous cell-cell interactions. Co-culture of mES cells and HepG2 cells decreased SOX17 expression of mES cells, and direct cell-cell contact further decreased SOX17 expression, indicating that co-culture with HepG2 cells inhibits endoderm differentiation through soluble factors and cell-cell contact. This method is simple and user-friendly and should be broadly useful to study cell shapes and cell-cell interactions.

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mentioning

confidence: 99%

Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures

Torisawa

Mosadegh

Cavnar

et al. 2011

Tissue Engineering Part C: Methods

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“…These technologies can produce a high resolution pattern, but they are inconvenient to form 3D structures and expensive to change patterns [10].…”

Section: Introductionmentioning

confidence: 99%

Feasibility study of a biocompatible pneumatic dispensing system using mouse 3T3-J2 fibroblasts

Lee

Kim

2017

Micro and Nano Syst Lett

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This paper presents results for dispensing living cells using a pneumatic dispensing system to verify the feasibility of using this system to fabricate biomaterials. Living cells (i.e., mouse 3T3-J2 fibroblast) were dispensed with different dispensing pressures in order to evaluate the effect of dispensing process on cell viability and proliferation. Based on the results of a live-dead assay, more than 80% of cell viability has been confirmed which was reasonably similar to that in the control group. Furthermore, measurement of cell metabolic activity after dispensing confirmed that the dispensed cell proliferated at a rate comparable to that of the control group. These results demonstrate that the pneumatic dispensing system is a promising tool for fabrication of biomaterials.

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“…Methods to geometrically separate different cell types in culture (patterning) enable broad studies of physical and chemical cues in cell biology. Most of the current approaches for patterning cell layers [1][2][3][4] depend on depositing cell-adhesion proteins on surfaces or using microfabricated stencils for selective growth on substrates. In contrast, here we present a method to rapidly pattern cell monolayers in situ, i.e., cells in culture, by removing cells in selected regions of the monolayer.…”

Section: Introductionmentioning

confidence: 99%

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

Kashyap

Cors

Lovchik

et al. 2016

JoVE

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The microfluidic probe (MFP) facilitates performing local chemistry on biological substrates by confining nanoliter volumes of liquids. Using one particular implementation of the MFP, the hierarchical hydrodynamic flow confinement (hHFC), multiple liquids are simultaneously brought in contact with a substrate. Local chemical action and liquid shaping using the hHFC, is exploited to create cell patterns by locally lysing and removing cells. By utilizing the scanning ability of the MFP, user-defined patterns of cell monolayers are created. This protocol enables rapid, real-time and spatially controlled cell patterning, which can allow selective cell-cell and cell-matrix interaction studies.

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Patterning multiple cell types in co-cultures: A review

Cited by 88 publications

References 90 publications

Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures

Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures

Feasibility study of a biocompatible pneumatic dispensing system using mouse 3T3-J2 fibroblasts

Rapid Subtractive Patterning of Live Cell Layers with a Microfluidic Probe

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