Patterning microfluidic device wettability with spatially-controlled plasma oxidation

Kim, Samuel; Sukovich, David J.; Abate, Adam R.

doi:10.1039/c5lc00626k

Cited by 69 publications

(80 citation statements)

References 46 publications

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“…PDMS devices were bonded to PDMS slabs by treating both with oxygen plasma for 60 s at 1 mbar of pressure in a plasma cleaner. Bonded devices were incubated at 65 °C for 36–48 hours prior to use38.…”

Section: Methodsmentioning

confidence: 99%

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Sukovich

Lance

Abate

2017

Sci Rep

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Genetic heterogeneity is an important feature of many biological systems, but introduces technical challenges to their characterization. Even with the best modern instruments, only a small fraction of DNA molecules present in a sample can be read, and they are recovered in the form of short, hundred-base reads. In this paper, we introduce 3dPCR, a method to sort DNA molecules with sequence specificity. 3dPCR allows heterogeneous populations of DNA to be sorted to recover long targets for deep sequencing. It is valuable whenever a target sequence is rare in a mixed population, such as for characterizing mutations in heterogeneous cancer cell populations or identifying cells containing a specific genetic sequence or infected with a target virus.

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Section: Methodsmentioning

confidence: 99%

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Sukovich

Lance

Abate

2017

Sci Rep

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“…The microfluidic devices are fabricated using soft lithography in poly(dimethylsiloxane) (PDMS) [25]. SU-8 masters are fabricated by photolithography and used to mold PDMS devices by mixing PDMS polymer and cross-linker at a ratio of 11:1, pouring over the master, degassing to remove air bubbles, and baking at 75 o C for 4 h to solidify.…”

Section: Methodsmentioning

confidence: 99%

Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry

Lance

Sukovich

Stedman

et al. 2016

Virol J

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BackgroundViruses are incredibly diverse organisms and impact all forms of life on Earth; however, individual virions are challenging to study due to their small size and mass, precluding almost all direct imaging or molecular analysis. Moreover, like microbes, the overwhelming majority of viruses cannot be cultured, impeding isolation, replication, and study of interesting new species. Here, we introduce PCR-activated virus sorting, a method to isolate specific viruses from a heterogeneous population. Specific sorting opens new avenues in the study of uncultivable viruses, including recovering the full genomes of viruses based on genetic fragments in metagenomes, or identifying the hosts of viruses.MethodsPAVS enables specific sorting of viruses with flow cytometry. A sample containing a virus population is processed through a microfluidic device to encapsulate it into droplets, such that the droplets contain different viruses from the sample. TaqMan PCR reagents are also included targeting specific virus species such that, upon thermal cycling, droplets containing the species become fluorescent. The target viruses are then recovered via droplet sorting. The recovered virus genomes can then be analyzed with qPCR and next generation sequencing.Results and ConclusionsWe describe the PAVS workflow and demonstrate its specificity for identifying target viruses in a heterogeneous population. In addition, we demonstrate recovery of the target viruses via droplet sorting and analysis of their nucleic acids with qPCR.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0655-7) contains supplementary material, which is available to authorized users.

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“…PDMS), but modification of the surface is still needed in order to i) further increase or decrease the contact angle between the solid of the channel walls and the working fluids (Jankowski et al, 2013); ii) pattern the device with hydrophobic (or hydrophilic) areas in order to, e.g. produce multiple droplets (Abate et al, 2010b;Kim S.C. et al, 2015); iii) shield the microchannel walls from the deposition of unwanted molecules and from the intake of swelling solvents (Abate et al, 2008;Lee et al, 2003;Zhou et al, 2012); or to iv) increase the adherence of the mammalian cells to the solid substrate (Zuchowska et al, 2016). Easy and durable methods for the modification of the microchannels in PDMS and hybrid PDMS glass devices are available.…”

Section: Surface Chemistrymentioning

confidence: 99%

“…hydrophilic modification by acrylic monomer that can bind to the glassy sol-gel coating (Abate et al, 2010b). Alternatively, the device can be patterned by treating parts of the microfluidic channel network with oxygen plasma or corona discharge-ionized oxygen species produced in those processes bombard the exposed PDMS surface and oxidize the surface, rendering the natively hydrophobic PDMS hydrophilic (Filla et al, 2011;Kim et al, 2015).…”

Section: Surface Chemistrymentioning

confidence: 99%

Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

Opalski

Kamiński

Garstecki

2019

KONA

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Emulsion-a liquid dispersed in another liquid-is in many respects very similar to granular matter. In the early 2000s a new technology-droplet microfluidics-began emerging from the wider field of microfluidics. Droplet microfluidics was quickly established as a discipline of science and engineering and has been used for the generation of highly uniform emulsions. The last few years have brought significant advances to the field, directed towards a wide range of applications in material sciences-from synthesis of nanoparticles in droplets to assembling complex droplets and using droplets as templates for ordered materials, with applications in food, cosmetic and diagnostic industries. Droplet microfluidic platforms are also successfully used as analytical tools in molecular biology and biochemistry, in e.g. high-throughput screening, digital assays, encapsulation of single cells, sequencing technologies, and in point-of-care diagnostic applications. This article provides an accessible overview of the physical phenomena observed in multiphase flow at the microscale and the techniques in droplet microfluidics systems. We also present the most interesting applications and potential further directions of research in this fascinating young field of science and engineering.

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Patterning microfluidic device wettability with spatially-controlled plasma oxidation

Cited by 69 publications

References 46 publications

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Sequence specific sorting of DNA molecules with FACS using 3dPCR

Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry

Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions

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