Patterned PCL/PGS Nanofibrous Hyaluronic Acid-Coated Scaffolds Promote Cellular Response and Modulate Gene Expression Profiles

Abstract

Chronic wounds impose a significant burden on individuals and healthcare systems, necessitating the development of advanced wound management strategies. Tissue engineering, with its ability to create scaffolds that mimic native tissue structures and promote cellular responses, offers a promising approach. Electrospinning, a widely used technique, can fabricate nanofibrous scaffolds for tissue regeneration. In this study, we developed patterned nanofibrous scaffolds using a blend of poly(ε-caprolactone) (PCL) and poly(glycerol sebacate) (PGS), known for their biocompatibility and biodegradability. By employing a mesh collector, we achieved a unique fiber orientation pattern that emulated the natural tissue architecture. The average fiber diameter of PGS/PCL collected on aluminum foil and on mesh was found to be 665.2 ± 4 and 404.8 ± 16 nm, respectively. To enhance the scaffolds' bioactivity and surface properties, it was coated with hyaluronic acid (HA), a key component of the extracellular matrix known for its wound-healing properties. The HA coating improved the scaffold hydrophilicity and surface wettability, facilitating cell attachment, spreading, and migration. Furthermore, the HA-coated scaffold exhibited enhanced biocompatibility, promoting cell viability and proliferation. High-throughput RNA sequencing was performed to analyze the influence of the fabricated scaffold on the gene expression levels of endothelial cells. The top-upregulated biological processes and pathways include cell cycle regulation and cell proliferation. The results revealed significant alterations in gene expression profiles, indicating the scaffold's ability to modulate cellular functions and promote wound healing processes. The developed scaffold holds great promise for advanced wound management and tissue regeneration applications. By harnessing the advantages of aligned nanofibers, biocompatible polymers, and HA coating, this scaffold represents a potential solution for improving wound healing outcomes and improving the quality of life for individuals suffering from chronic wounds.

Zeolitic imidazolate framework-8 encapsulated with Mo-based polyoxometalates as surfaces with antibacterial activity against Escherichia coli

“…In 1995, a breakthrough emerged in the field of nanoporous materials with the introduction of metal–organic frameworks (MOFs). 8 MOFs are primarily composed of metal nodes or clusters attached by coordination bonds to organic linkers, constituting a highly customizable and adjustable porous structure and an exceptionally high surface area ranging from 1000 to 10 000 m 2 g −1 . 8–10 Zeolite-imidazolate frameworks (ZIFs) are a subclass of MOFs that were first introduced in 2006.…”

“…8 MOFs are primarily composed of metal nodes or clusters attached by coordination bonds to organic linkers, constituting a highly customizable and adjustable porous structure and an exceptionally high surface area ranging from 1000 to 10 000 m 2 g −1 . 8–10 Zeolite-imidazolate frameworks (ZIFs) are a subclass of MOFs that were first introduced in 2006. 11,12 They are composed of metal nodes (M) tetrahedrally coordinated with 4 imidazolate-based organic linkers (IM), each acting as a bridge between two metal nodes, typically a transition metal ( e.g.…”