Patterned biofunctional designs of thermoresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment

Hatakeyama, Hideyuki; Kikuchi, Akihiko; Yamato, Masayuki; Okano, Teruo

doi:10.1016/j.biomaterials.2007.04.019

Cited by 91 publications

(81 citation statements)

References 38 publications

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“…These results indicate that the binding of cell integrins to modified RGDS on cell culture substrates can be dissociated by a mild environmental stimulation of temperature, without enzymatic or chemical treatment, due to the shielding of hydrated polymer chains. These findings are important to control the specific interaction between proteins and cells, and the subsequent "on-off" regulation of their function [56,[58][59][60]. Furthermore, because the method allows a serum-free cell culture and a trypsin-free cell harvest to be realized, it should be an attractive cell culture method that requires no mammalian-sourced component.…”

Section: Fabricating Biomolecule On Temperature-responsive Polymer Momentioning

confidence: 99%

Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

2012

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Abstract:In the past two decades, as a novel approach for tissue engineering, cell sheet engineering has been proposed by our laboratory. Poly(N-isopropylacrylamide) (PIPAAm), which is a well-known temperature-responsive polymer, has been grafted on tissue culture polystyrene (TCPS) surfaces through an electron beam irradiated polymerization. At 37 °C, where the PIPAAm modified surface is hydrophobic, cells can adhere, spread on the surface and grow to confluence. By decreasing temperature to 20 °C, since the surface turns to hydrophilic, cells can detach themselves from the surface spontaneously and form an intact cell sheet with extracellular matrix. For obtaining a temperature-induced cell attachment and detachment, it is necessary to immobilize an ultra thin PIPAAm layer on the TCPS surfaces. This review focuses on the characteristics of PIAPAm modified surfaces exhibiting these intelligent properties. In addition, PIPAAm modified surfaces giving a rapid cell-sheet recovery has been further developed on the basis of the characteristic of the PIPAAm surface. The designs of temperature-responsive polymer layer have provided an enormous potential to fabricate clinically applicable regenerative medicine.

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Section: Fabricating Biomolecule On Temperature-responsive Polymer Momentioning

confidence: 99%

Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

2012

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“…This might be part of the foreign body reaction that occurs after implantation of a skeletal muscle construct in vivo (Saxena et al, 2001;Kamelger et al, 2004;Luttikhuizen et al, 2006). An alternative technique which does not require a 3D scaffold is cell sheet technology (Tsuda et al, 2004;Nishida et al, 2004;Yang et al, 2005Yang et al, , 2006Hatakeyama et al, 2007;Ohashi et al, 2007;Masuda et al, 2008). Although originally developed for myocardial TE purposes (Masuda et al, 2008), the technique has found its way to other fields of TE, such as liver (Ohashi et al, 2007) and cornea (Nishida et al, 2004), and is promising for engineering facial muscles.…”

Section: Scaffoldsmentioning

confidence: 99%

Current opportunities and challenges in skeletal muscle tissue engineering

Koning¹,

Harmsen²,

Luyn³

et al. 2009

J Tissue Eng Regen Med

144

108

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The purpose of this article is to give a concise review of the current state of the art in tissue engineering (TE) of skeletal muscle and the opportunities and challenges for future clinical applicability. The endogenous progenitor cells of skeletal muscle, i.e. satellite cells, show a high proneness to muscular differentiation, in particular exhibiting the same characteristics and function as its donor muscle. This suggests that it is important to use an appropriate progenitor cell, especially in TE facial muscles, which have a exceptional anatomical and fibre composition compared to other skeletal muscle. Muscle TE requires an instructive scaffold for structural support and to regulate the proliferation and differentiation of muscle progenitor cells. Current literature suggests that optimal scaffolding could comprise of a fibrin gel and cultured monolayers of muscle satellite cells obtained through the cell sheet technique. Tissue-engineered muscle constructs require an adequate connection to the vascular system for efficient transport of oxygen, carbon dioxide, nutrients and waste products. Finally, functional and clinically applicable muscle constructs depend on adequate neuromuscular junctions with neural cells. To reach this, it seems important to apply optimal electrical, chemotropic and mechanical stimulation during engineering and discover other factors that influence its formation. Thus, in addition to approaches for myogenesis, we discuss the current status of strategies for angiogenesis and neurogenesis of TE muscle constructs and the significance for future clinical use.

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“…For example, changing the surface energy of the substrate via thermally-induced effects has been demonstrated [86][87][88] , and in Paper V we demonstrated an entirely new electroactive cell release method using ferroelectric films.…”

Section: Cell Detachmentmentioning

confidence: 95%

Operating Organic Electronics via Aqueous Electric Double Layers

Toss¹

2015

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The field of organic electronics emerged in the 1970s with the discovery of conducting polymers. With the introduction of plastics as conductors and semiconductors came many new possibilities both in production and function of electronic devices. Polymers can often be processed from solution and their softness provides both the possibility of working on flexible substrates, and various advantages in interfacing with other soft materials, e.g. biological samples and specimens. Conducting polymers readily partake in chemical and electrochemical reactions, providing an opportunity to develop new electrochemically driven devices, but also posing new problems for device engineers.The work of this thesis has focused on organic electronic devices in which aqueous electrolytes are an active component, but still operating in conditions where it is desirable to avoid electrochemical reactions. Interfacing with aqueous electrolytes occurs in a wide variety of settings, but we have specifically had biological environments in mind as they necessarily involve the presence of water. The use of liquid electrolytes also provides the opportunity to deliver and change the device electrolyte continuously, e.g. through microfluidic systems, which could then be used as a dynamic feature and/or be used to introduce and change analytes for sensors. Of particular interest is the electric double layer at the interface between the electrolyte and other materials in the device, specifically its sensitivity to charge reorganization and high capacitance.The thesis first focuses on organic field effect transistors gated through aqueous electrolytes. These devices are proposed as biosensors with the transistor architecture providing a direct transduction and amplification so that it can be electrically read out. It is discussed both how to distinguish between the various operating mechanisms in electrolyte thin film transistors and how to choose a strategy to achieve the desired mechanism. Two different strategies to suppress ion penetration into, and thus electrochemical doping of, the organic semiconductor are presented.The second focus of the thesis is on polarization of ferroelectric polymer films through electrolytes. A model for the interaction between the remnant ferroelectric charge in the polymer film and the mobile ionic charges of the electrolyte is presented, and verified experimentally. The reorientation of the ferroelectric polarization via the electric double layer is also demonstrated in a regenerative medicine application; the ferroelectric polarization is shown to affect cell binding, and is used as a gentle method to non-destructively detach cells from a culture substrate. Populärvetenskaplig SammanfattningUpptäckten av ledande polymerer på 1970-talet blev startskottet för forskningsområdet ledande plaster och senare också för organisk elektronik. Möjligheten att använda polymera material, det vill säga plaster, som elektrisk ledare och halvledare i elektroniska komponenter innebär en rad olika fördelar med avseende ...

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Patterned biofunctional designs of thermoresponsive surfaces for spatiotemporally controlled cell adhesion, growth, and thermally induced detachment

Cited by 91 publications

References 38 publications

Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering

Current opportunities and challenges in skeletal muscle tissue engineering

Operating Organic Electronics via Aqueous Electric Double Layers

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