Patterned Assembly of Genetically Modified Viral Nanotemplates via Nucleic Acid Hybridization

Abstract

The patterning of nanoparticles represents a significant obstacle in the assembly of nanoscale materials and devices. In this report, cysteine residues were genetically engineered onto the virion surface of tobacco mosaic virus (TMV), providing attachment sites for fluorescent markers. To pattern these viruses, labeled virions were partially disassembled to expose 5' end RNA sequences and hybridized to virus-specific probe DNA linked to electrodeposited chitosan. Electron microscopy and RNAase treatments confirmed the patterned assembly of the virus templates onto the chitosan surface. These findings demonstrate that TMV nanotemplates can be dimensionally assembled via nucleic acid hybridization.

Mechanistic study of the hydrothermal reduction of palladium on the Tobacco mosaic virus

“…The outer surface of the virus can be described as a simple repetition of identical 2.3×3.5 nm 2 unit surfaces 2 . The virion coat protein has been engineered via site directed mutagenesis 73 so that one or two cysteine groups can be exposed on the outer surface of each coat protein. The subsequent coating of this genetically modified TMV as well as wild type TMV with various metals and metal sulfide nanoparticles has been widely reported.…”

Bridging the Bio-Electronic Interface with Biofabrication

“…Chitosan can be deposited on various microscale geometries -such as in microchannels and on non-planar surfaces 20,15 . The films can also be modified with other polymers and a variety of proteins, DNA, nanoparticles, and redox-active molecules for novel properties 21,22,23 . In bioMEMS devices, chitosan films have been used for drug delivery, redox and small molecule detection, biocatalysis, and cell studies 20,23,24,25 .…”

Genetically Modified Collagen‐like Triple Helix Peptide as Biomimetic Template THIS CHAPTER HAS BEEN RETRACTED

“…The tobacco mosaic virus (TMV), a rod-shaped biomolecular template, has been applied for various metal coatings, however, to achieve accurate control of the length with low dispersity is not an easy task. [12][13][14] The other type-DNA biomolecular templates-have defined lengths determined by the number of nucleic acids, but they lack conformational rigidity. The tendency of supertwisting of the double-helix DNA structure makes it difficult to obtain rigid and linear nanowires.…”