Patterned artificial juxtacrine stimulation of cells by covalently immobilized insulin

Ito, Yoshihiro; Kondo, Satoshi; Chen, Guoping; Imanishi, Yukio

doi:10.1016/s0014-5793(97)00045-8

Cited by 46 publications

(31 citation statements)

References 19 publications

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“…NGF photochemical fixation was performed using a phenyl‐azido group, a method developed by Matsuda and Sugawara46 and modified by Ito and coworkers for immobilization of growth factors in particular 41, 42, 47. The procedure consisted of three main steps (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…A common approach for this protein immobilization is the use of arylazido‐containing compounds, which can react nonspecifically with UV light by creating singlet nitrenes that undergo insertion into CH, NH, and other bonds 44, 45. Using this method, proteins have been fixed to many substrates such as poly(vinyl alcohol), polystyrene, poly(ethylene terephthalate), and chitosan 41, 42, 46–49. Here we extended this approach for the surface decoration of PPy with NGF.…”

Section: Introductionmentioning

confidence: 99%

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Nerve growth factor‐immobilized polypyrrole: Bioactive electrically conducting polymer for enhanced neurite extension

Gómez

Schmidt

2006

J Biomedical Materials Res

284

260

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Biomaterials that present multiple stimuli are attractive for a number of biomedical applications. In particular, electrical and biological cues are important factors to include in interfaces with neurons for applications such as nerve conduits and neural probes. Here, we report the combination of these two stimuli, by immobilizing nerve growth factor (NGF) on the surface of the electrically conducting polymer polypyrrole (PPy). NGF was immobilized using an intermediate linker provided by a layer of polyallylamine conjugated to an arylazido functional group. Upon exposure to UV light and activation of the azido groups, NGF was fixed to the substrate. Three different surface concentrations were obtained (0.21-0.98 ng/mm 2 ) and similar levels of neurite extension were observed on immobilized NGF as with soluble NGF. Additionally, electrical stimulation experiments were conducted with the modified polymer and revealed a 50% increase in neurite outgrowth in PC12 cells compared to experiments without electrical stimulation. This novel modification of PPy provides both electrical and biological stimulation, by presenting tethered growth factors and only producing a small decrease in the material's properties (conductivity ~10 S cm −1 ) when compared to other modification techniques (conductivity ~10 −3 -10 −6 S cm −1 .

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nerve growth factor‐immobilized polypyrrole: Bioactive electrically conducting polymer for enhanced neurite extension

Gómez

Schmidt

2006

J Biomedical Materials Res

284

260

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“…Thus, NGF immobilized to substrata is effective in inducing neurite extension, turning and sprouting [35][36][37][38][39][40]. Other growth factors including insulin, epidermal growth factor and vascular endothelial growth factor have also been immobilized in active form on a variety of substrates [41][42][43]. A common approach for this protein immobilization is the use of arylazido-containing compounds, which can react nonspecifically with UV light by creating singlet nitrenes that undergo insertion into C-H, N-H and other bonds [44,45].…”

Section: Introductionmentioning

confidence: 99%

“…A common approach for this protein immobilization is the use of arylazido-containing compounds, which can react nonspecifically with UV light by creating singlet nitrenes that undergo insertion into C-H, N-H and other bonds [44,45]. Using this method, proteins have been fixed to many substrates such as poly(vinyl alcohol), polystyrene, poly(ethylene terephthalate), and chitosan [41,42,[46][47][48][49].…”

Section: Introductionmentioning

confidence: 99%

Immobilized nerve growth factor and microtopography have distinct effects on polarization versus axon elongation in hippocampal cells in culture

Gómez¹,

Lu²,

Chen³

et al. 2007

Biomaterials

176

178

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“…The micropatterning technique has also been used to investigate the effect of immobilized growth factor 19 and anticoagulant polymer on poly(ethylene terephthalate) film. 20 This technique enabled regulation of cell patterning by detachment on a biomaterial and will be useful to compare microscopically qualitative surface properties under the same conditions.…”

Section: Cell Attachment and Detachmentmentioning

confidence: 99%

Effect of protein and cell behavior on pattern-grafted thermoresponsive polymer

Chen

Imanishi

Ito

1998

J. Biomed. Mater. Res.

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A thermoresponsive copolymer, poly(Nisopropylacrylamide-co-acrylic acid), was coupled with azidoaniline. The azidophenyl-derivatized copolymer was grafted in a specific pattern on a polystyrene matrix by photolithography. The surface micropattern appeared and disappeared interchangeably, as observed under a phase-contrast microscope, by varying the temperature between 10 degrees C and 37 degrees C. The copolymer-grafted polystyrene surface was hydrophobic at 37 degrees C and hydrophilic at 10 degrees C. Albumin and fibronectin adsorption on the matrix was investigated using the fluorescent-labeling method. Fibronectin adsorbed onto both the grafted and nongrafted regions, while albumin adsorbed more onto the nongrafted regions than the grafted regions. Protein adsorption did not affect surface wettability. Mouse fibroblast STO cells were cultured on tissue culture plates pattern-grafted with the thermoresponsive copolymer. Fibronectin adsorption enhanced cell spreading, while albumin reduced it. When the temperature was lowered, the cells selectively detached from the surface areas grafted with the thermoresponsive copolymer when cultured in serum-free medium; the cells partially detached from these areas when cultured in serum-containing medium. The effect of serum proteins on cell detachment was similar to that caused by a mixture of albumin and fibronectin. Albumin adsorption did not affect the detachment of cells, while fibronectin adsorption inhibited it. The results of the present study indicate that a pattern-grafted, thermoresponsive, azidophenyl-derivatized copolymer can effectively facilitate selective cell detachment under some conditions such as serum-free culture or preadsorption of albumin. The pattern-grafting technique will be useful for qualitative microscopic comparison of surfaces prepared differently on one chip under the same conditions.

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Patterned artificial juxtacrine stimulation of cells by covalently immobilized insulin

Cited by 46 publications

References 19 publications

Nerve growth factor‐immobilized polypyrrole: Bioactive electrically conducting polymer for enhanced neurite extension

Nerve growth factor‐immobilized polypyrrole: Bioactive electrically conducting polymer for enhanced neurite extension

Immobilized nerve growth factor and microtopography have distinct effects on polarization versus axon elongation in hippocampal cells in culture

Effect of protein and cell behavior on pattern-grafted thermoresponsive polymer

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