Patternable Gelatin Methacrylate/PEDOT/Polystyrene Sulfonate Microelectrode Coatings for Neuronal Recording

Bansal, Mahima; Vyas, Yukti; Aqrawe, Zaid; Raos, Brad; Cheah, Ernest; Montgomery, Johanna M.; Wu, Zimei; Svirskis, Darren

doi:10.1021/acsbiomaterials.2c00231

Cited by 4 publications

(3 citation statements)

References 61 publications

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“…The electrical conductivity and electrochemical impedance spectroscopy (EIS) of the PEDOT:PSS scaffold are two important properties as they directly affect the current flow through the scaffold under ES operation, thereby impacting the cell behavior and neuronal recordings. − ,, Therefore, the conductivity of PG3 was measured in the film and scaffold state using the four-point probe and two probe methods, respectively (Figure d). The conductivity of the PG3 scaffold was found to be 5.6 × 10 –6 S/cm, which was 3 orders of magnitude lower than that of the PG3 thin film (4.56 × 10 –4 S/cm).…”

Section: Resultsmentioning

confidence: 99%

Biophysical Electrical and Mechanical Stimulations for Promoting Chondrogenesis of Stem Cells on PEDOT:PSS Conductive Polymer Scaffolds

Liu

Lin

et al. 2023

Biomacromolecules

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The investigation of the effects of electrical and mechanical stimulations on chondrogenesis in tissue engineering scaffolds is essential for realizing successful cartilage repair and regeneration. The aim of articular cartilage tissue engineering is to enhance the function of damaged or diseased articular cartilage, which has limited regenerative capacity. Studies have shown that electrical stimulation (ES) promotes mesenchymal stem cell (MSC) chondrogenesis, while mechanical stimulation (MS) enhances the chondrogenic differentiation capacity of MSCs. Therefore, understanding the impact of these stimuli on chondrogenesis is crucial for researchers to develop more effective tissue engineering strategies for cartilage repair and regeneration. This study focuses on the preparation of poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) conductive polymer (CP) scaffolds using the freeze-drying method. The scaffolds were fabricated with varying concentrations (0, 1, 3, and 10 wt %) of (3-glycidyloxypropyl) trimethoxysilane (GOPS) as a crosslinker and an additive to tailor the scaffold properties. To gain a comprehensive understanding of the material characteristics and the phase aggregation phenomenon of PEDOT:PSS scaffolds, the researchers performed theoretical calculations of solubility parameters and surface energies of PSS, PSS-GOPS, and PEDOT polymers, as well as conducted material analyses. Additionally, the study investigated the potential of promoting chondrogenic differentiation of human adipose stem cells by applying external ES or MS on a PEDOT:PSS CP scaffold. Compared to the group without stimulation, the group that underwent stimulation exhibited significantly up-regulated expression levels of chondrogenic characteristic genes, such as SOX9 and COL2A1. Moreover, the immunofluorescence staining images exhibited a more vigorous fluorescence intensity of SOX9 and COL II proteins that was consistent with the trend of the gene expression results. In the MS experiment, the strain excitation exerted on the scaffold was simulated and transformed into stress. The simulated stress response showed that the peak gradually decreased with time and approached a constant value, with the negative value of stress representing the generation of tensile stress. This stress response quantification could aid researchers in determining specific MS conditions for various materials in tissue engineering, and the applied stress conditions could be further optimized. Overall, these findings are significant contributions to future research on cartilage repair and biophysical ES/MS in tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Biophysical Electrical and Mechanical Stimulations for Promoting Chondrogenesis of Stem Cells on PEDOT:PSS Conductive Polymer Scaffolds

Liu

Lin

et al. 2023

Biomacromolecules

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“…In this case, PSS‐coated GelMA hydrogels were obtained by soaking GelMA hydrogels in a PEDOT:PSS solution. [ 41 ]…”

Section: Preparation Of Chsmentioning

confidence: 99%

Seamless Integration of Conducting Hydrogels in Daily Life: From Preparation to Wearable Application

Imani,

Dodda,

Yoon

et al. 2024

Advanced Science

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Conductive hydrogels (CHs) have received significant attention for use in wearable devices because they retain their softness and flexibility while maintaining high conductivity. CHs are well suited for applications in skin‐contact electronics and biomedical devices owing to their high biocompatibility and conformality. Although highly conductive hydrogels for smart wearable devices are extensively researched, a detailed summary of the outstanding results of CHs is required for a comprehensive understanding. In this review, the recent progress in the preparation and fabrication of CHs is summarized for smart wearable devices. Improvements in the mechanical, electrical, and functional properties of high‐performance wearable devices are also discussed. Furthermore, recent examples of innovative and highly functional devices based on CHs that can be seamlessly integrated into daily lives are reviewed.

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“…The third set of methods incorporates additives into suspensions of PEDOT that form cross-linked networks upon UV light exposure. − These methods typically present the smallest trade-offs, with some achieving high conductivity, and others remarkable stretchability. However, the best methods require the use of advanced synthetic techniques that may be out of reach for many users.…”

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confidence: 99%

Photography-Inspired Patterned Vapor Phase Polymerization of Conductive PEDOT on Rigid and Stretchable Substrates

Edmunds,

Urbina,

Fishman

et al. 2024

ACS Materials Lett.

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The conductive polymers poly(3,4-ethylenedioxythiophene) (PEDOT) and polypyrrole are widely used in organic optoelectronic, bioelectronic, and electrochromic applications. However, a general process for forming highly conductive films with high spatial resolution on various substrates is lacking. This work describes a technique for forming PEDOT and polypyrrole films with high spatial resolution using vapor-phase polymerization (VPP). The process, reminiscent of classical black-and-white photography, employs a photosensitive VPP initiating film. UV light selectively deactivates the film, allowing oxidative polymerization in unexposed regions. Substrates are modified with N-[3-(trimethoxysilyl)propyl]aniline to enhance adhesion. Optimizing the initiating film composition and exposure conditions achieves conductive lines as narrow as 15 μm with PEDOT conductivities over 1000 S cm −1 . Mechanistic studies using X-ray photoelectron spectroscopy, UV−visible spectroscopy, and Fourier transform infrared spectroscopy suggest a critical role of oxidative decomposition by photoinitiated ligand-to-metal charge transfer. The resulting conductive patterns exhibit abrasion resistance on glass, retain conductivity when stretched on elastomers, and enable two-color electrochromic displays.

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Patternable Gelatin Methacrylate/PEDOT/Polystyrene Sulfonate Microelectrode Coatings for Neuronal Recording

Cited by 4 publications

References 61 publications

Biophysical Electrical and Mechanical Stimulations for Promoting Chondrogenesis of Stem Cells on PEDOT:PSS Conductive Polymer Scaffolds

Biophysical Electrical and Mechanical Stimulations for Promoting Chondrogenesis of Stem Cells on PEDOT:PSS Conductive Polymer Scaffolds

Seamless Integration of Conducting Hydrogels in Daily Life: From Preparation to Wearable Application

Photography-Inspired Patterned Vapor Phase Polymerization of Conductive PEDOT on Rigid and Stretchable Substrates

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