Pattern recognition receptors in grass carp Ctenopharyngodon idella: II. Organization and expression analysis of NOD-like receptors

Xu, Tianbing; Liao, Zhiwei; Su, Jianguo

doi:10.1016/j.dci.2020.103734

Cited by 19 publications

(9 citation statements)

References 50 publications

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“…In contrast, stx gene was not detected in any of the 24 strains. In relation to the species as shown in Table 3, the results showed that A. veronii strains had a higher number of positive strains (25) for different genes, followed by A. dhakensis (23), A. piscicola (18), and A. caviae (15), while A. media (9) and A. jandaei (5) had a smaller number of positive strains.…”

Section: Presence Of Virulence Factorsmentioning

confidence: 99%

“…This process is characterized by the activation of different types of caspase enzymes and contributes to the activation of pro-inflammatory cytokines, recruits more immune cells, and finally activates an inflammatory cascade in the tissue (14). In the last years, few studies have investigated the expression of several innate immune-related genes against A. hydrophila, A. veronii, and Aeromonas salmonicida in different cell lines or animal models (mice and fish) (12,(21)(22)(23)(24). The majority of the studies has been performed with cell lines obtained from animals, with the exception of two cell lines (T-84 and WLR-68) obtained from the human colon (12,24).…”

Section: Introductionmentioning

confidence: 99%

“…Both genes form a complex resulting in the formation of the activator protein-1 (AP-1), which is a transcription factor family implicated in critical cell functions such as the inflammatory response (29). Moreover, after A. hydrophila and A. salmonicida infections in fish, the activation of the gene expression that encodes pro-inflammatory cytokines like TNF-a and interleukin 8 (IL-8), as well as chemokines, i.e., the C-C motif ligand 20 (CCL20) involved in the recruitment of lymphocytes and dendritic cells, was demonstrated (21,23,30,31). However, the C-C motif chemokine ligand 2 (CCL2) has not been studied in Aeromonas, despite it being an interesting chemokine well studied in infections produced by other bacteria like Vibrio spp., where it plays a role in the recruitment of monocytes, T cells such as CD4+ and CD8+, and dendritic cells (32).…”

Section: Introductionmentioning

confidence: 99%

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Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

Fernández-Bravo

Figueras

2022

Front. Immunol.

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Aeromonas are autochthonous bacteria of aquatic environments that are considered to be emerging pathogens to humans, producing diarrhea, bacteremia, and wound infections. Genetic identification shows that 95.4% of the strains associated with clinical cases correspond to the species Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). However, few studies have investigated the human immune response against some Aeromonas spp. such as A. hydrophila, Aeromonas salmonicida, and A. veronii. The present study aimed to increase the knowledge about the innate human immune response against six Aeromonas species, using, for the first time, an in vitro infection model with the monocytic human cell line THP-1, and to evaluate the intracellular survival, the cell damage, and the expression of 11 immune-related genes (TLR4, TNF-α, CCL2, CCL20, JUN, RELA, BAX, TP53, CASP3, NLRP3, and IL-1β). Transcriptional analysis showed an upregulated expression of a variety of the monocytic immune-related genes, with a variable response depending upon the Aeromonas species. The species that produced the highest cell damage, independently of the strain origin, coincidentally induced a higher expression of immune-related genes and corresponded to the more prevalent clinical species A. dhakensis, A. veronii, and A. caviae. Additionally, monocytic cells showed an overexpression of the apoptotic and pyroptotic genes involved in cell death after A. dhakensis, A. caviae, and Aeromonas media infection. However, the apoptosis route seemed to be the only way of producing cell damage and death in the case of the species Aeromonas piscicola and Aeromonas jandaei, while A. veronii apparently only used the pyroptosis route.

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Section: Presence Of Virulence Factorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

Fernández-Bravo

Figueras

2022

Front. Immunol.

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“…Additional category of NLRC3-like proteins consisting of a distinctive PYD-FISNA-NACHT structure was cloned in zebrafish and blunt snout bream [ 94 , 99 ]. Recently, NLRC3-like proteins in grass carp have been explicitly classified into four categories according to their domain architecture [100] : (i) having PYD, FISNA, NACHT and LRR domains, (ii) possessing FISNA-NACHT-LRR structure, (iii) containing FISNA, NACHT, LRR and SPRY-PRY-SNTX domains and (iv) consisting PYD, FISNA and NACHT domains, no LRRs. The ubiquitous expressions of NLRC3-like genes have been reported in various teleosts, namely zebrafish [20] , Japanese flounder [39,89], blunt snout bream [98] , goldfish [101] and Atlantic salmon [92] .…”

Section: Diversity Of Nlrsmentioning

confidence: 99%

Teleost NOD-like receptors and their downstream signaling pathways: A brief review

Chuphal

Rai²,

Roy³

2022

Fish and Shellfish Immunology Reports

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“…were further subjected to whole genome shotgun (WGS) sequencing on the Illumina NovaSeq and PacBio Sequel sequencing platforms (Bio-Project ID of bacteria genome sequencing raw data: PRJNA785292). The Step MCScanX software package from TBtools was used to analyze the collinearity of the two genomes [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense

Zhang

Shu

et al. 2022

Microbiome

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Background Herbivorous insects acquire their gut microbiota from diverse sources, and these microorganisms play significant roles in insect hosts’ tolerance to plant secondary defensive compounds. Camellia weevil (Curculio chinensis) (CW) is an obligate seed parasite of Camellia oleifera plants. Our previous study linked the CW’s gut microbiome to the tolerance of the tea saponin (TS) in C. oleifera seeds. However, the source of these gut microbiomes, the key bacteria involved in TS tolerance, and the degradation functions of these bacteria remain unresolved. Results Our study indicated that CW gut microbiome was more affected by the microbiome from soil than that from fruits. The soil-derived Acinetobacter served as the core bacterial genus, and Acinetobacter sp. was putatively regarded responsible for the saponin-degradation in CW guts. Subsequent experiments using fluorescently labeled cultures verified that the isolate Acinetobacter sp. AS23 can migrate into CW larval guts, and ultimately endow its host with the ability to degrade saponin, thereby allowing CW to subsist as a pest within plant fruits resisting to higher concentration of defensive chemical. Conclusions The systematic studies of the sources of gut microorganisms, the screening of taxa involved in plant secondary metabolite degradation, and the investigation of bacteria responsible for CW toxicity mitigation provide clarified evidence that the intestinal microorganisms can mediate the tolerance of herbivorous insects against plant toxins.

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Pattern recognition receptors in grass carp Ctenopharyngodon idella: II. Organization and expression analysis of NOD-like receptors

Cited by 19 publications

References 50 publications

Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.

Teleost NOD-like receptors and their downstream signaling pathways: A brief review

Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense

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