Pattern of the Occurrence of Tomato spotted wilt virus in Jeonnam Province

Abstract

Tomato spotted wilt virus (TSWV) was occurred at 8 areas including Naju, Suncheon, Younggwang, Youngam, and Shinan in Jeonnam province and the crops of Younggwang were severely damaged by TSWV. The hot pepper (Capsicum annuum), bell pepper (Capsicum annuum var. angulosum) and tomato (Solanum lycopersicon) in greenhouse and hot pepper in open field were infected by TSWV. Especially, hot pepper was severely damaged by TSWV infection. The survey data indicated that 1.1−30% in the nursery field at Naju, Suncheon, and Jangheung were infected by TSWV. Plants were infected by TSWV from early June to August. However, TSWV-infected seedlings from nursery fields showed the disease symptoms from May after transplanting. In pepper greenhouses, Frankliniella occidentalis was more dominant insect vector than Frankliniella intonsa. But in open field, the population of insect vector was opposed to greenhouse. In addition, the removal of weeds was able to delay the incidence of TSWV via side-window of greenhouse in Winter. Taken together, the control of weed and insect vector nearby side-window of greenhouse is important to prevent TSWV infection of plants.

Life Cycle-Based Host Range Analysis for Tomato Spotted Wilt Virus in Korea

“…Total RNA was extracted from collected samples with easy-spin Total RNA Extraction Kit (Intron Biotechnology, Seongnam, Korea) according to the manufacturer's manual. TSWV infection was confirmed by RT-PCR with AMV Reverse Transcriptase (for cDNA synthesis, Promega, Madison, WI, USA), GoTaq DNA Polymerase (for PCR, Promega), and a TSWV-specific primer set (TSWV-6F, 5'-GAGATTCT-CAGAATTCCCAGT-3'; TSWV-6R, 5'-AGAGCAATC-GTGTCAATTTTATTC-3'), as described in a previous study (Tables 1-3) (Ko et al, 2013).…”

Incidence of Viral Diseases on Red Pepper in Yeongyang-Gun, Gyeongbuk Provice

“…CMV Broad bean wilt virus 2 (BBWV2) (Lee , 2015;Ko , 2013 (Cho , 2005;Kim , 2008, Ko , 2013Lee , 2015), TSWV . .…”