Pattern of TAAR5 Expression in the Human Brain Based on Transcriptome Datasets Analysis

Vaganova, Anastasia N.; Murtazina, Ramilya Z.; Shemyakova, T. S.; Prjibelski, Andrey D.; Katolikova, N.; Gainetdinov, Raul R.

doi:10.3390/ijms22168802

Cited by 12 publications

(15 citation statements)

References 58 publications

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“…A low level of expression of all functional TAARs was also observed in the human hippocampus. These data are consistent with a previous study, where we found a predominantly limbic pattern of distribution of TAAR5 in the human brain in a similar transcriptomic analysis [ 30 ]. A similar conclusion was reached in the transcriptomic analysis performed by an independent group that found the expression of all TAARs, with TAAR5 being the most highly expressed, in the limbic brain areas, including the hippocampus [ 31 ].…”

Section: Discussionsupporting

confidence: 94%

Expression of Trace Amine-Associated Receptors in the Murine and Human Hippocampus Based on Public Transcriptomic Data

Katolikova

Vaganova

Efimova

et al. 2022

Cells

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Hippocampus is one of the neurogenic zones where adult neurogenesis takes place. This process is quite complex and has a multicomponent regulation. A family of G protein-coupled trace amine-associated receptors (TAARs) was discovered only in 2001, and most of them (TAAR2-TAAR9) were primarily considered olfactory. Recent studies have shown, however, that they are also expressed in the mouse brain, particularly in limbic formations, and can play a role in the regulation of emotional behaviors. The observations in knockout mice indicate that at least two members of the family, TAAR2 and TAAR5, have an impact on the regulation of adult neurogenesis. In the present study, we analyzed the expression of TAARs in the murine and human hippocampus using public RNAseq datasets. Our results indicate a low but detectable level of certain TAARs expression in the hippocampal cells in selected high-quality transcriptomic datasets from both mouse and human samples. At the same time, we observed the difference between humans, where TAAR6 expression was the highest, and murine samples, where TAAR1, TAAR2, TAAR3, TAAR4 and TAAR5 are more pronouncedly expressed. These observations provide further support to the data gained in knockout mice, indicating a role of TAARs in the regulation of adult neurogenesis in the hippocampus.

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Section: Discussionsupporting

confidence: 94%

Expression of Trace Amine-Associated Receptors in the Murine and Human Hippocampus Based on Public Transcriptomic Data

Katolikova

Vaganova

Efimova

et al. 2022

Cells

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“…Only two samples with low TAAR6 expression were identified in this dataset (and only one of them demonstrated an expression level above the cut-off), and in both cases, TAAR6 expression was revealed in the nuclear transcriptome. In addition, as was described previously, DRD2 mRNA was predominately associated with the nucleus and HTR1A mRNA was mostly detected in the cytoplasm [ 48 , 49 ] ( Figure 3 ).…”

Section: Resultssupporting

confidence: 78%

Public Transcriptomic Data Meta-Analysis Demonstrates TAAR6 Expression in the Mental Disorder-Related Brain Areas in Human and Mouse Brain

et al. 2022

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G protein-coupled trace amine-associated receptors (TAAR) recognize different classes of amine compounds, including trace amines or other exogenous and endogenous molecules. Yet, most members of the TAAR family (TAAR2-TAAR9) are considered olfactory receptors involved in sensing innate odors. In this study, TAAR6 mRNA expression was evaluated in the brain transcriptomic datasets available in the GEO, Allen Brain Atlas, and GTEx databases. Transcriptomic data analysis demonstrated ubiquitous weak TAAR6 mRNA expression in the brain, especially in the prefrontal cortex and nucleus accumbens. RNA sequencing of isolated cells from the nucleus accumbens showed that the expression of TAAR6 in some cell populations may be more pronounced than in whole-tissue samples. Curiously, in D1 and D2 medium spiny neurons of the nucleus accumbens, TAAR6 expression was co-regulated with genes involved in G protein-coupled receptor signaling. However, in cholinergic interneurons of the nucleus accumbens, TAAR6 expression was not associated with the activation of any specific biological process. Finally, TAAR6 expression in the mouse prefrontal cortex was validated experimentally by RT-PCR analysis. These data demonstrated that TAAR6 is expressed at low levels in the human and mouse brain, particularly in limbic structures involved in the pathogenesis of mental disorders, and thus might represent a new pharmacotherapeutic target.

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“…Additionally, transcriptomic analysis of TAAR5 expression in the mouse cerebellum revealed low expression of this receptor in several datasets. Recent detailed transcriptomic analysis of TAAR5 expression in the human brain also showed ubiquitous low TAAR5 expression in the cerebellum and other brain areas including the cortical and limbic brain areas, the amygdala and the hippocampus, the nucleus accumbens, the thalamus, the hypothalamus, the basal ganglia, the substantia nigra, and the white matter 28 , 29 . Similar conclusions were achieved independently in another transcriptomic analysis study where expression of all TAARs was found in several brain areas including cerebellum and brainstem with TAAR5 appeared to be having the highest expression level 29 .…”

Section: Discussionmentioning

confidence: 99%

Role of the trace amine associated receptor 5 (TAAR5) in the sensorimotor functions

Калинина

Ptukha

Горяинова

et al. 2021

Sci Rep

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Classical monoamines are well-known modulators of sensorimotor neural networks. However, the role of trace amines and their receptors in sensorimotor function remains unexplored. Using trace amine-associated receptor 5 knockout (TAAR5-KO) mice, that express beta-galactosidase mapping its localization, we observed TAAR5 expression in the Purkinje cells of the cerebellum and the medial vestibular nucleus, suggesting that TAAR5 might be involved in the vestibular and motor control. Accordingly, in various behavioral tests, TAAR5-KO mice demonstrated lower endurance, but better coordination and balance compared to wild-type controls. Furthermore, we found specific changes in striatal local field potentials and motor cortex electrocorticogram, such as a decrease in delta and an increase in theta oscillations of power spectra, respectively. The obtained data indicate that TAAR5 plays a considerable role in regulation postural stability, muscle force, balance, and motor coordination during active movements, likely via modulation of monoaminergic systems at different levels of sensorimotor control involving critical brain areas such as the brainstem, cerebellum, and forebrain.

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Pattern of TAAR5 Expression in the Human Brain Based on Transcriptome Datasets Analysis

Cited by 12 publications

References 58 publications

Expression of Trace Amine-Associated Receptors in the Murine and Human Hippocampus Based on Public Transcriptomic Data

Expression of Trace Amine-Associated Receptors in the Murine and Human Hippocampus Based on Public Transcriptomic Data

Public Transcriptomic Data Meta-Analysis Demonstrates TAAR6 Expression in the Mental Disorder-Related Brain Areas in Human and Mouse Brain

Role of the trace amine associated receptor 5 (TAAR5) in the sensorimotor functions

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