Pattern of seroreactivity against feline foamy virus proteins in domestic cats from Germany

Bleiholder, Anne; Mühle, Michael; Hechler, Torsten; Bevins, Sarah N.; VandeWoude, Sue; Denner, Joachim; Löchelt, Martin

doi:10.1016/j.vetimm.2011.06.007

Cited by 23 publications

(37 citation statements)

References 36 publications

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“…Presumably, BFV is better suppressed in calves than in sheep, resulting in the observed pattern in Env-specific seroreactivity. In addition, our current findings confirm the diagnostic value of Gag and Bet as antigens for BFV serodiagnostics, as previously shown also for FFV (21,52,53).…”

Section: Discussionsupporting

confidence: 91%

Similar Patterns of Infection with Bovine Foamy Virus in Experimentally Inoculated Calves and Sheep

Materniak

Hechler

Löchelt

et al. 2013

J Virol

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bFoamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bovine FV (BFV) are not available. In this study, we conducted experimental infections of natural (cattle) and heterologous (sheep) hosts with the BFV 100 isolate and monitored infection patterns in both hosts during the early phase postinoculation as well as after long-term infection. Four calves and six sheep inoculated with BFV 100 showed no signs of pathology but developed persistent infection, as confirmed by virus rescue, consistent detection of BFV-specific antibodies, and presence of viral DNA. In both hosts, antibodies against BFV Gag and Bet appeared early after infection and persisted at high and stable levels while seroreactivity toward Env was consistently detectable only in BFV-infected sheep. Interestingly, the BFV proviral DNA load was highest in lung, spleen, and liver and moderate in leukocytes, while salivary glands contained either low or undetectable DNA loads in calves or sheep, respectively. Additionally, comparison of partial BFV sequences from inoculum and infected animals demonstrated very limited changes after long-term infection in the heterologous host, clearly less than those found in BFV field isolates. The persistence of BFV infection in both hosts suggests full replication competence of the BFV 100 isolate with no requirement of genetic adaptation for productive replication in the authentic and even in a heterologous host.

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Section: Discussionsupporting

confidence: 91%

Similar Patterns of Infection with Bovine Foamy Virus in Experimentally Inoculated Calves and Sheep

Materniak

Hechler

Löchelt

et al. 2013

J Virol

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“…prevalence of this infection in cat populations [25,26]. FFV can establish latent infection in the host and then induce syncytial cell formation and apoptosis in cultured cells [27,28].…”

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confidence: 99%

Feline Foamy Virus Adversely Affects Feline Mesenchymal Stem Cell Culture and Expansion: Implications for Animal Model Development

Arzi

Kol

Murphy

et al. 2015

Stem Cells and Development

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Mesenchymal stem cells (MSCs) are a promising therapeutic option for various immune-mediated and inflammatory disorders due to their potent immunomodulatory and trophic properties. Naturally occurring diseases in large animal species may serve as surrogate animal models of human disease, as they may better reflect the complex genetic, environmental, and physiologic variation present in outbred populations. We work with naturally occurring diseases in large animal species to better understand how MSCs work and to facilitate optimal translation of MSC-based therapies. We are investigating the use of MSC therapy for a chronic oral inflammatory disease in cats. During our efforts to expand fat-derived feline MSCs (fMSCs), we observed that*50% of the cell lines developed giant foamy multinucleated cells in later passages. These morphologic alterations were associated with proliferation arrest. We hypothesized that the cytopathic effects were caused by infection with a retrovirus, feline foamy virus (FFV). Using transmission electron microscopy, polymerase chain reaction, and in vitro assays, we determined that syncytial cell formation and proliferation arrest in fMSCs were caused by FFV strains that were highly homologous to previously reported FFV strains. We determined that the antiretroviral drug, tenofovir, may be used to support ex vivo expansion and salvage of FFV-infected fMSC lines. MSC lines derived from specific pathogen-free cats do not appear to be infected with FFV and may be a source of allogeneic fMSCs for clinical application. FFV infection of fMSC lines may hinder large-scale expansion of autologous MSC for therapeutic use in feline patients.

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“…In addition to the immunisation studies the TM protein of the FFV was also used to establish a diagnostic tool based on the detection of TM protein-specific antibodies in infected animals. 57,58 Furthermore, one goat and eight rats were immunised with these proteins and binding and neutralizing antibodies were analyzed. In all animals binding peptide of FV Env, designated Elp, is an important component of FFV and PFV particles.…”

Section: Immunisation With the Tm Protein Of Foamy Virusesmentioning

confidence: 99%

Pattern of seroreactivity against feline foamy virus proteins in domestic cats from Germany

Abstract: The prevalence of feline foamy virus (FFV, spumaretrovirinae)

Cited by 23 publications

References 36 publications

Similar Patterns of Infection with Bovine Foamy Virus in Experimentally Inoculated Calves and Sheep

Similar Patterns of Infection with Bovine Foamy Virus in Experimentally Inoculated Calves and Sheep

Feline Foamy Virus Adversely Affects Feline Mesenchymal Stem Cell Culture and Expansion: Implications for Animal Model Development

Immunising with the transmembrane envelope proteins of different retroviruses including HIV-1

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