Pattern of ROS generation and interconversion on wet stigmas in basal and divergent angiosperms

Breygina, Maria; Luneva, O. G.; Schekaleva, Olga; Lazareva, N. N.; Babushkina, Ksenia; Kirilyuk, Igor A.

doi:10.1007/s10725-023-01033-w

Cited by 2 publications

(3 citation statements)

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“…Stigma maturity was assessed according to flower appearance and was divided into 4 stages(for details and appearance see [ 19 ]), where stage 1 was a juvenile stigma, stage 2 was a pre-mature stigma, stage 3 was a fully mature unpollinated stigma, and stage 4 was a pollinated stigma (the next day after pollination). Exudate was collected from stigmas of all stages by a “cap method”: A pipette tip containing 10 μL (tobacco) or an Eppendorf test tube containing 200 µL (lily) of distilled water was applied on the pistil and incubated for 30 min to wash the exudate off the stigma (25 °C).…”

Section: Methodsmentioning

confidence: 99%

“…The authors believe that these proteins cleave polymers of stigma exudate to oligomers and monomers, thereby facilitating their uptake by growing PTs. Among the low molecular weight exudate components, reactive oxygen species (ROS) are the most studied [ 18 , 19 ]. In tobacco stigmas, as the pistil matured, the level of both O • 2 − and H 2 O 2 in the exudate decreased markedly [ 20 ], whereas in lily, the level of total ROS increased [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among the low molecular weight exudate components, reactive oxygen species (ROS) are the most studied [ 18 , 19 ]. In tobacco stigmas, as the pistil matured, the level of both O • 2 − and H 2 O 2 in the exudate decreased markedly [ 20 ], whereas in lily, the level of total ROS increased [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Plant Hormone and Fatty Acid Screening of Nicotiana tabacum and Lilium longiflorum Stigma Exudates

Breygina,

Kochkin,

Voronkov

et al. 2023

Biomolecules

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Pollen germination in vivo on wet stigmas is assisted by the receptive fluid—stigma exudate. Its exact composition is still unknown because only some components have been studied. For the first time, hormonal screening was carried out, and the fatty acid (FA) composition of lipid-rich (Nicotiana tabacum) and sugar-rich (Lilium longiflorum) exudates was studied. Screening of exudate for the presence of plant hormones using HPLC-MS revealed abscisic acid (ABA) in tobacco stigma exudate at the two stages of development, at pre-maturity and in mature stigmas awaiting pollination, increasing at the fertile stage. To assess physiological significance of ABA on stigma, we tested the effect of this hormone in vitro. ABA concentration found in the exudate strongly stimulated the germination of tobacco pollen, a lower concentration had a weaker effect, increasing the concentration did not increase the effect. GC-MS analysis showed that both types of exudate are characterized by a predominance of saturated FAs. The lipids of tobacco stigma exudate contain significantly more myristic, oleic, and linoleic acids, resulting in a higher unsaturation index relative to lily stigma exudate lipids. The latter, in turn, contain more 14-hexadecenoic and arachidic acids. Both exudates were found to contain significant amounts of squalene. The possible involvement of saturated FAs, ABA, and squalene in various exudate functions, as well as their potential relationship on the stigma, is discussed.

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Section: Methodsmentioning

confidence: 99%