Pattern of prevalent Hepatitis B virus genotypes in Zaria, Nigeria

Ahmad, Abdurrahman Elfulaty; Bakari, AG; Musa, Bolanle O. P.; Mustapha, Sagir; Jamoh, Bello Yusuf; Abdullahi, Idris Nasir; Tahir, Mohammed Ibrahim; Olatunji, Abdulqadri Olarenwaju; Maishanu, Sumayya Hamza; Suleiman, Abubakar; Tolulope, Afolaranmi; Hawkins, Claudia; Sagay, Atiene S.; Zoakah, AI; Olayinka, Adebola

doi:10.4103/npmj.npmj_59_19

Cited by 19 publications

(13 citation statements)

References 41 publications

(42 reference statements)

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“…Some investigators have also suggested that patient's fulminant hepatitis is more frequent in patients with genotype D infection [41], with higher HBeAg positivity seen in patients infected with genotype D adds credence to this assertion. Interestingly, HBV mono-genotype infection was shown to dominate over mixed genotype infection that opposed to a study conducted in speci c parts of Africa in which HBV mixed-genotype infections are predominant [42]. The observed HBV mixed-genotypes infection in this study may be due to possible recombination between genotypes or migration ows.…”

Section: Discussioncontrasting

confidence: 93%

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Hamida¹,

Raja²,

Petros³

et al. 2021

Preprint

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Aim To determine serological markers and genotypes profiles of HBV isolates in Central Eritrea. Materials & methods A total of 191 HBsAg sero-positive samples were randomly selected for the study from 23,232 screened blood donors from 2015 to 2017. Enzyme-linked immunosorbent assay was used to perform HBV serological markers screening, while genotypes were determined using type-specific primer-based multiplex-nested PCR. Results The median age of infected blood donors was 28.9 ± 10.26 years. Of 191 HBsAg reactive serum samples, 77.5% (148/191) were sero-positive for HBcAb-total, among which 99.3% (147/148) and 0.7% (1/148) were sero-positive for HBsAg and HBsAb, respectively. Interestingly, among 147 HBcAb-total/HBsAg reactive samples, 16 (10.9%) and 131 (77.9%) were sero-positive for HBeAg and HBeAb, respectively. For genotyping, 73 HBV isolates were successfully amplified and genotyped, with 59 (80.8%) had a mono-genotype and 14 (19.2%) had a mixed-genotype infection. Among HBV isolates with mono-genotypes: 39 (53.4%) D; 10 (13.7%) E; 6 (8.2%) A and 4 (5.5%) C. While five mixed-genotypes comprised: 6 (8.2%) C/D; 3 (4.1%) C/D/E; 2 (2.7%) each with genotype A/D and D/E; and 1 (1.4%) genotype B/D. Conclusion HBV genotype D is the predominant genotype, either as a HBV mono- or mixed-genotype infection, among blood donors in Eritrea.

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Section: Discussioncontrasting

confidence: 93%

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Hamida¹,

Raja²,

Petros³

et al. 2021

Preprint

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“…Our nding of single-genotype predominance also agrees with a study in Egypt using INNO-LiPA, which reported that 87% of patients harboured singlegenotype infection, most commonly genotype D [25]. However, these ndings contrast with those from Iraq and Nigeria, in which mixed-genotype infections were documented in 75 and 82.6% of patients, respectively [19,26].…”

Section: Discussionsupporting

confidence: 90%

“…This method appears to have higher sensitivity for detecting mixed genotypes, and it is simple and cost-effective for large population studies with a high accuracy rate of 93% [17]. [18][19][20][21][22]. These ndings con rmed the variability of different methods of HBV-DNA detection in relation to HBsAg positivity, and that the variability could be in uenced by whether patients have chronic infection [23].…”

Section: Discussionmentioning

confidence: 98%

Prevalence of Hepatitis B Virus Genotypes Among Patients with Liver Disease in Eritrea

Hamida¹,

Raja²,

Petros³

et al. 2020

Preprint

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BackgroundHepatitis B virus (HBV) is a blood-borne hepatotrophic virus and a major causative agent of liver disease. The virus is highly endemic in African countries, and five genotypes (A–E) have been identified. This study aimed to establish the most prevalent genotypes of HBV among liver disease patients from different geographical regions of Eritrea, an East African multi-ethnic country.MethodsThis study included 293 Eritrean liver disease patients who were hepatitis B surface antigen (HBsAg)-positive. Enzyme-linked immunosorbent assay (ELISA)-based serological screening and multiplex-nested PCR using type-specific primer-based genotyping were performed to determine the prevalence of genotypes in the Eritrean population.ResultsThe mean (± standard deviation) age was 41.66 ± 13.84 years; 213 (72.7%) patients were males and 80 (27.3%) were females. The median (interquartile range) of HBV DNA and alanine aminotransferase levels were 3.47 (1.66) log IU/ml and 28 (15.3) IU/L, respectively. All patients’ sera were HBsAg- and anti-HBc-total positive; 20 (6.8%) were HBeAg-positive/anti-HBe-negative, 242 (82.6%) were HBeAg-negative/anti-HBe-positive, and 31 (10.6%) had neither HBeAg nor anti-HBe according to the ELISA screening test. Of the 293 patients, only 122 (41.6%) were positive for HBV DNA, 57.38% had a single genotype, and 42.62% had a mixed HBV genotype infection. Irrespective of mode of occurrence, HBV genotype D (n = 26; 21.3%) was the predominant circulating genotype, followed by genotypes C (n = 21; 17.2%), E (n = 19; 15.6%), C/D (n = 16; 13.1%), and C/E (n = 13; 10.7%). Genotypes C/D/E (n = 9; 7.4%), A/D (n = 6; 4.9%), D/E (n = 5; 4.1%), A (n = 3; 2.5%), and B, A/E, B/E, and A/D/C (each with n = 1; 0.8%) were also present.ConclusionHBV in Eritrea is comprised of a mixture of genotypes A, B, C, D, and E separately or in combinations. Our findings demonstrated that in Eritrea, the most prevalent HBV genotype in Eritrea is genotype D among theliver disease patients with higher HBeAg positivity. This is the first study of HBV genotyping based on PCR methods in Eritrea.

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“…This is in concordance with findings from Taiwan, in which single genotype B infection was most common 19 , and with a study in Egypt, which reported that 87% of patients harbored single-genotype infection, most commonly genotype D 20 . On the contrary, studies from Iraq and Nigeria documented that mixed-genotype infections were present in 75% and 82.6% of patients, respectively 21 , 22 . When mixed and single infections were considered together, we found that genotype D had the highest prevalence, followed by genotypes C, E, C/D, C/E, C/D/E, A/D, D/E, A, B, A/E, B/E, and A/D/C.…”

Section: Discussionmentioning

confidence: 90%

Prevalence of hepatitis B virus genotypes among patients with liver disease in Eritrea

Hamida

Raja

Petros

et al. 2021

Sci Rep

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Eritrea is an East African multiethnic country with an intermediate endemicity for hepatitis B. Our aim was to establish the most prevalent genotypes of hepatitis B virus (HBV) among patients with liver disease. A total of 293 Eritrean patients with liver disease who were hepatitis B surface antigen (HBsAg) positive were enrolled. All sera were tested for liver transaminases, HBV DNA viral load, and hepatitis B seromarkers including HBsAg, anti-HBcAb (total), HBeAg, and anti-HBeAb. Those reactive for HBsAg and anti-HBc (total) were further tested for HBV genotyping. The median (interquartile range) of HBV DNA viral load and ALT levels were 3.47 (1.66) log IU/mL and 28 (15.3) IU/L, respectively. Using type-specific primer-based genotyping method, 122/293 (41.6%) could be genotyped. Irrespective of mode of occurrence, HBV genotype D (21.3%) was the predominant circulating genotype, followed by genotypes C (17.2%), E (15.6%), C/D (13.1%), and C/E (10.7%). Genotypes C/D/E (7.4%), A/D (4.9%), D/E (4.1%), A (2.5%), and B, A/E, B/E, and A/D/C (0.8%) were also present. HBV in Eritrea is comprised of a mixture of HBV genotypes. This is the first study of HBV genotyping among patients with liver disease in Eritrea.

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Pattern of prevalent Hepatitis B virus genotypes in Zaria, Nigeria

Cited by 19 publications

References 41 publications

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Genotyping and sero-virological characterisation of hepatitis B virus-infected blood donors in Central Eritrea

Prevalence of Hepatitis B Virus Genotypes Among Patients with Liver Disease in Eritrea

Prevalence of hepatitis B virus genotypes among patients with liver disease in Eritrea

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