Pattern of microorganism from burn wounds and their trends of susceptibility to antibiotics in tertiary care center

Shrivastava, Gunjan; Bhatambare, GS; Lunawat, Ajay; Patel, Kamlesh; Maheshwari, Tanmay

doi:10.4103/2347-9019.183229

Cited by 2 publications

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“…In the recent years, Pseudomonas aeruginosa has overtaken Staphylococcus aureus in causing wound infections [3]. In the past three years, some of the Indian studies shown Pseudomonas sp as the most predominant isolate from wound infections (Gunjam Shrivastava et al, 2016 [4], Vivek Kulkami et al, 2015 [5] and Richa Gupta et al, 2016 [6]. In one of the studies from Maharashtra, Sapana G Mundhada et al, 2015 [7] reported Pseudomonas aeruginosa as second dominant strain isolated, wherein Klebsiella sp was the most predominant isolation.…”

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confidence: 99%

Antibiotic Susceptibility Pattern of <i>Pseudomonas aeruginosa</i> Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Babu¹

2018

IJIDT

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Multiple antibiotic resistance in bacterial populations is a pervasive and growing clinical problem, which is recognized as a threat to public health. Drug resistance to Pseudomonas sp. has spread to such a level irrespective of the type of patients, that, its pattern of distribution and antibiotic resistance needs to be studied in detail, especially in trauma patients. Of the Gram negative bacilli, Pseudomonas aeruginosa has been of particular interest, the incidence of which in wound infection has increased compared to a decade back. The objective of this study was to know the antibiotic susceptibility pattern of Pseudomonas aeruginosa isolates from wound infections. Out of the total of 1404 cases from which pus samples collected, 204 (14.5%) yielded Pseudomonas aeruginosa isolates. Among the antibiotics tested, Imipenem was the most sensitive drug, showing susceptibility in 81.3% of the isolates, followed by Piperacillin + tazobactam (76.4%), Meropenem (70.5%) and Piperacillin (67.6%). Fifteen (7.4%) multidrug resistant strains were reported out of the 204 isolations. Increase in resistance to aminoglycosides and fluoroquinolones in this study, prompted evaluation of antibiotic susceptibility pattern of Pseudomonas isolates from clinical samples at regular intervals.

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Section: Introductionmentioning

confidence: 99%

Antibiotic Susceptibility Pattern of <i>Pseudomonas aeruginosa</i> Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Babu¹

2018

IJIDT

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Bacteriological and PCR Detection of Pvl, Msra and Meca Genes Among Staphylococcus Aureus Isolated From Burn Wounds

Muhamad¹,

Anwar²,

Omer³

2019

JSMC

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Background Methicillin-resistant Staphylococcus aureus is a pathogen that is associated with nosocomial and community- burn wound infection. S aureus produces Panton-Valentine -Leukocidin which results in the destruction of leukocytes. Resistance of S. aureus to macrolides, lincosamides, and streptogramin B is associated with the presence of an efflux pump, encoded by Methionine Sulfoxide Reductase A (msrA or msrB) genes. Objectives To isolate, determine the antibiotic susceptibility pattern, and to detect the presence of pvl and msrA genes from Staphylococcus aureus, isolated from burn wounds. Materials and Methods A total of 423 burn wound samples (218 from hospitalized and 205 from outpatients) were cultivated on different bacteriological media. Isolates were identified and S. aureus were further subjected to antibiotic susceptibility testing using disk diffusion method. Susceptibility to methicillin, oxacillin or cefoxitin, were used to determine methicillin-resistant Staphylococcus aureus strains. Polymerase chain reaction was used to detect mecA, pvl, and msrA genes in S. aureus isolates. Results Bacterial growth was detected from 170 (77.9%) of hospital samples and from 183 (89.26%) community-burn wounds. The predominant isolates were Gram-negative bacilli (71.76%) among hospitalized patients followed by S. aureus (22.35%). From the community samples, Staphylococcus epidermidis was the predominant isolate (86.9%), while few species of other Gram-positive organisms were also detected but no Gram-negatives were isolated. Among the 41 S. aureus isolates, the prevalence of methicillin-resistant S. aureus strains determined by oxacillin disk diffusion method was 58.53%, 65.85% by cefoxitin, whereas, 87.8% were positive for mecA gene by PCR. Pvl was detected in 3 (7.31%), while mrsA gene was detected among 17 (41.46%) of S. aureus isolates. Conclusions Infection with Methicillin-resistant Staphylococcus aureus was common in burn wounds. The prevalence of msrA gene among nosocomial and community-burn wound isolates of S. aureus was high, while few S. aureus isolates were found to carry pvl gene.

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Pattern of microorganism from burn wounds and their trends of susceptibility to antibiotics in tertiary care center

Cited by 2 publications

References 1 publication

Antibiotic Susceptibility Pattern of <i>Pseudomonas aeruginosa</i> Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Antibiotic Susceptibility Pattern of <i>Pseudomonas aeruginosa</i> Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Bacteriological and PCR Detection of Pvl, Msra and Meca Genes Among Staphylococcus Aureus Isolated From Burn Wounds

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Pattern of microorganism from burn wounds and their trends of susceptibility to antibiotics in tertiary care center

Cited by 2 publications

References 1 publication

Antibiotic Susceptibility Pattern of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Antibiotic Susceptibility Pattern of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Bacteriological and PCR Detection of Pvl, Msra and Meca Genes Among Staphylococcus Aureus Isolated From Burn Wounds

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Antibiotic Susceptibility Pattern of <i>Pseudomonas aeruginosa</i> Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India

Antibiotic Susceptibility Pattern of <i>Pseudomonas aeruginosa</i> Isolates from Wound Infections in a Tertiary Care Centre in South Kerala, India