“…Extracts were used to detect the presence of secondary metabolites like alkaloids by mixing 2mL of extract with 1% HCl, followed by boiling and treatment with 6 drops of Mayer's reagent (Chhetri, 2008 ;Parekh, 2007;Evans, 1989), presence of phenol was detected by phenol and ellagic acid test (Mallikharjuna, 2007, Dey & Harbour, 1987, Evans, 1989, flavonoids by Alkaline reagent test (Mallikharjuna, 2007, Dey & Harbour, 1987, Evans, 1989 and flavonoid test (Mallikharjuna, 2007, Dey & Harbour, 1987, Evans, 1989, tannin by mixing crude extract with 2mL of 2% FeCl 3 solution (Mallikharjuna, 2007, Chhetri, 2008, Doss, 2009, lignin by treating crude extract with 2mL of furfuraldehyde (Mallikharjuna, 2007, Dey & Harbour, 1987, Evans, 1989, steroids by Salkowski's test (Mallikharjuna, 2007, Dey & Harbour, 1987, Evans, 1989, glycosides by mixing crude extract with 2mL of glacial acetic acid containing 1-2 drops of 2% sol of FeCl 3 followed by addition of 2mL of H 2 SO 4 (Krishnaiah, 2009;Edeoga, 2005;Doss, 2009), saponins by shaking the crude extract with water (Dey & Harbour, 1987;Evans, 1989), tepenoides by Salkowski test (Rajasekariah et al, 1991) and anthraquinone by mixing the crude extract with 2mL of chloroform and conc. H 2 SO 4 .…”