Pattern of expression of cyclin D1/CDK4 complex in human placenta during gestation

Falco, Maria De; Fedele, Vita; Cobellis, Luigi; Mastrogiacomo, Annunziata; Giraldi, D.; Leone, Stefania; Luca, Luigi De; Laforgia, Vincenza; Luca, Antonio De

doi:10.1007/s00441-004-0880-z

Cited by 34 publications

(24 citation statements)

References 29 publications

(32 reference statements)

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“…The presence of cyclins D1 and D3 in the nuclei of mesenchymal cells and labyrinthine trophoblast cells during late pregnancy might suggest their involvement in the maintenance of a welldifferentiated state. Similar to the situation in the human placenta (De Falco et al 2004), cyclins D1 and D3 were observed primarily in the endothelial cells. Since proliferation of endothelial cells is a prerequisite for the formation of new vessels and elongation of existing ones, cyclins D1 and D3 appear to be involved in the control of placental angiogenesis during the third trimester of gestation.…”

Section: Pcna and G1 Cyclinssupporting

confidence: 52%

Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta

Korgun

Unek

Herrera

et al. 2011

Histochem Cell Biol

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As cell cycle regulation is fundamental to the normal growth and development of the placenta, the aim of the present study was to determine the immunolocalizations of cell cycle related proteins, which have key roles in proliferation, differentiation and apoptosis during the development of the rat placenta. Here immunohistochemistry has been used to localize G1 cyclins (D1, D3, E), which are major determinants of proliferation, CIP/KIP inhibitors (p21, p27, p57), p53 as a master regulator and proliferating cell nuclear antigen in all cell types of the rat term placenta. The proportion of each cell type immunolabeled was counted. Cyclin D1 and cyclin D3 were present mostly in cells of the fetal aspect of the placenta, whereas the G1/S cyclin E was present only in the spongio- and labyrinthine trophoblast populations. Among the CIP/KIP inhibitors, p21 was present only in cells of the fetal aspect whereas p27 and p57 were found in all cell types studied. p53 was only found in a small proportion of cells with no co-localization of p53 and p21. The data suggest that the cells of the fetal side of the rat placenta still have some proliferation potential which is kept in check by expression of the CIP/KIP cell cycle inhibitors, whereas cells of the maternal aspect have lost this potential. Apoptosis is only marginal in the term rat placenta. In conclusion, proliferation and apoptosis in rat placental cells appears controlled mostly by the CIP/KIP inhibitors in late pregnancy.

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Section: Pcna and G1 Cyclinssupporting

confidence: 52%

Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta

Korgun

Unek

Herrera

et al. 2011

Histochem Cell Biol

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“…Double immunohistochemical labelling with cytokeratin 7 allowed clear identification of the EVT and their expression of the cell cycle regulators, thus ruling out any confusion with the large leucocyte population present in the decidua at early stages in gestation. Other studies on cell cycle regulators have either not reported on the EVT or not distinguished between proximal and distal cells of the cell columns (Deloia et al 1997;Bamberger et al 1999;Olvera et al 2001;De Falco et al 2004). The set of cell cycle regulators present in the cell column strongly suggests that the cells in both the proximal and distal parts do not acutely proliferate.…”

Section: Cip/kip Cell Cycle Inhibitorsmentioning

confidence: 92%

Location of cell cycle regulators cyclin B1, cyclin A, PCNA, Ki67 and cell cycle inhibitors p21, p27 and p57 in human first trimester placenta and deciduas

Korgun

Çelik-Özenci

Acar

et al. 2006

Histochem Cell Biol

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Although placental development and implantation depend on the coordination of trophoblast proliferation, differentiation and invasion, little is known about the cell cycle regulators that govern the control of these events. The hypothesis that the coordinated expression of cell cycle progression and inhibition factors will determine whether cytotrophoblasts proliferate or undergo cell cycle arrest or cell cycle exit allowing subsequent differentiation was tested. The cell cycle promotors cyclin A, cyclin B1, PCNA, Ki67 and the cell cycle inhibitors p21, p27 and p57 were immunolocalized in tissue sections of first trimester pregnancies (weeks 6 and 9-12). Double staining with cytokeratin 7 allowed unambiguous identification of extravillous cytotrophoblast (EVT) in the decidua. Villous cytotrophoblasts were immunolabelled for Ki67 and cyclin A but only few were stained with anti-cyclin B1. The syncytiotrophoblast was devoid of immunoreactivity for any of the cell cycle progression factors. It expressed especially p21, whereas p27 and p57 were predominantly found in villous cytotrophoblasts. PCNA, Ki67, cyclin A and cyclin B1 were immunolocalized in proximal and distal EVTs of anchoring villi and in EVT which had invaded the upper decidual segments. All EVTs strongly expressed p27 and p57, but not p21. These data clearly suggest different functions for p21, p27 and p57 in placental development with distinct roles for p21 and p57 in syncytiotrophoblast and EVT differentiation, respectively. p27 appears to be involved in both the processes. The results may also challenge the concept of differential mitotic activity in the proximal and distal parts of the first trimester cytotrophoblast cell column, but more functional studies are clearly needed. The presence of p27 and p57 in EVT cells, which invade the deciduas deeply, may account for the loss of mitogenic potential of these cells.

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“…22,23 Human and animal placental expression studies suggest that cell cycle genes may differ in expression or function in women with hypertension in pregnancy or small for gestational age (SGA). 24,25 …”

Section: Introductionmentioning

confidence: 99%

Polymorphisms in Inflammatory Genes are Associated with Term Small for Gestational Age and Preeclampsia

Harmon

Engel

et al. 2014

American J Rep Immunol

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Problem Inflammatory biomarkers are associated with preeclampsia (PE) and poor fetal growth; however, genetic epidemiologic studies have been limited by reduced gene coverage and the exclusion of African American mothers. Method of study Cases and controls (N = 1646) from a pregnancy cohort were genotyped for 503 tagSNPs in 40 genes related to inflammation. Gene-set analyses were stratified by race and were followed by a single SNP analysis within significant gene sets. Results Gene-level associations were found for IL6 and KLRD1 for term small for gestational age (SGA) among African Americans. LTA/TNF and TBX21 were associated with PE among European Americans. The strongest association was for PE among European Americans for an upstream regulator of TNF with RR = 1.8 (95% CI 1.1–2.7). Conclusion Although previous studies have suggested null associations, increased tagging and stratification by genetic ancestry suggests important associations between IL6 and term SGA for African Americans, and a TNF regulator and PE among European Americans (N = 149).

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Pattern of expression of cyclin D1/CDK4 complex in human placenta during gestation

Cited by 34 publications

References 29 publications

Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta

Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta

Location of cell cycle regulators cyclin B1, cyclin A, PCNA, Ki67 and cell cycle inhibitors p21, p27 and p57 in human first trimester placenta and deciduas

Polymorphisms in Inflammatory Genes are Associated with Term Small for Gestational Age and Preeclampsia

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