Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts

Becher, Rune; Kopperud, Hilde Molvig; Al, Ronald H.; Samuelsen, Jan Tore; Morisbak, Else; Dahlman, Hans Jørgen; Lilleaas, Edel; Dahl, Jon E.

doi:10.1016/j.dental.2005.05.013

Cited by 89 publications

(87 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been demonstrated that even low concentrations of 2-hydroxyethyl methacrylate (HEMA) can significantly alter the morphology of human gingival fibroblasts 27) and primary pulp sells 28) . Comparable observations were reported by Becher and co-authors: extracts of some compomers as well as monomers TEGMA, HEMA and GDMA have the capacity to induce cell death in macrophages in vitro 29) . It is important to consider that insufficient curing or long setting time of materials may increase cytotoxicity.…”

Section: Discussionsupporting

confidence: 66%

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

et al. 2015

View full text Add to dashboard Cite

The study aimed to 1) evaluate the cytotoxicity of luting cements: Hoffmann's Zinc Phosphate (Hoffmann's ZP), GC Fuji Plus Resin Modified Glass Ionomer (Fuji Plus RMGI) and 3M ESPE RelyX Unicem Resin Cement (RelyX Unicem RC) and 2) test if pre-washing reduces the cements' cytotoxicity. In vitro human gingival fibroblast (HGF) culture model was chosen. The cytotoxicity was evaluated by MTT test, the cell viability -by staining the cells with AO/EB dye mixture. The means±SD of Cell Survival Ratio (CSR%) were compared among different cement types under two testing conditions, with or without cement pre-washing. The CSR%s were compared by ANOVA and linear multiple regression (LMR). Hoffmann's ZPC was less cytotoxic, while Fuji Plus RMGIC and RelyX Unicem RC were more cytotoxic (ANOVA, p<0.001). The type of cement and cement pre-washing jointly explained 90% of cell survival (LMR, p<0.001, adjusted squared R=0.889). The commonly used luting cements such as Hoffmann's ZP, Fuji Plus RMGI and RelyX Unicem RC may have a cytotoxic potential.

show abstract

Section: Discussionsupporting

confidence: 66%

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The results of this study are in agreement with previous in vitro investigations in which the cytotoxicity of resinous components was evaluated using the MTT assay (7,17). The extracts obtained from the Adper Single Bond 2 adhesive system were capable of reducing cellular viability in macrophages in vitro, ratifying the study of Becher et al (17).…”

Section: Discussionsupporting

confidence: 81%

“…TEGDMA has been described as being more toxic than HEMA in the MTT assay (17). However, Falconi et al (7) warned that low concentrations of HEMA could significantly alter the morphology of cells, such as human gingival fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that HEMA and TEGDMA released from resin dental materials have a cytotoxic action (7,17). TEGDMA has been described as being more toxic than HEMA in the MTT assay (17).…”

Section: Discussionmentioning

confidence: 99%

“…The pattern of apoptotic response, cellular glucose, oxygen consumption and gene expression after exposure to the various adhesive compounds has been used to evaluate cytotoxicity in previous studies (12,17,20) and can be used to assess cytopathic effects of multicomponent extracts from cured dental adhesives such as used in this study. The use of different methods to evaluate the cytotoxicity of dental materials may results in data that's enables to provide a knowledge base able to clarify how dentin adhesive affects cell behavior.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro potential cytotoxicity of an adhesive system to alveolar macrophages

et al. 2009

View full text Add to dashboard Cite

The purpose of this study was to evaluate the potential cytotoxicity of Adper Single Bond 2 (SB) simplified etch-and-rinse adhesive system in alveolar macrophage cultures, as a function of the post-polymerization time and duration of immersion in the culture medium for preparation of extracts, by observing the levels of nitric oxide (NO) release and cell survival rate (MTT assay). Wistar rat alveolar macrophages were exposed to 200 µL of extracts obtained from 24-or 72-h immersion of adhesive samples in culture medium (RPMI), immediately or 24 h after polymerization. Fresh RPMI and E. coli lipopolysaccharides were used as negative and positive controls, respectively. The cells were placed in a humidified incubator for 24 h. The results were analyzed by the Student's-t test (α=5%). The amount of NO produced and viable cells were significantly different (p<0.05) between the experimental and the control groups, showing that, irrespective of the post-polymerization time and duration of immersion in the culture medium, the adhesive system caused intense cytotoxicity to the macrophages. The cytotoxic effects were not statistically different (p<0.05) among the experimental groups. In conclusion, chemical components released from SB in aqueous environment were highly toxic to cell culture and thus an inflammatory pulpal response should be considered during the clinical application of dental adhesives.

show abstract

Effect of recrystallization on the molecular mobility of a copolymer of vinylidene fluoride and hexafluoropropylene

Malyshkina

Бессонова

Suljanov

2010

J of Applied Polymer Sci

View full text Add to dashboard Cite

Relaxation processes in copolymers of vinylidene fluoride with hexafluoropropylene (93/7) were studied by means of a dielectric method. The initial extruded film was recrystallized by free heating to temperatures above the melting point and by subsequent cooling. This increased both the perfection of the crystal phase and the degree of crystallinity. The impact of recrystallization on both the relaxation times (s's) and the activation parameters of the local mobility (b process) and microBrownian cooperative mobility in amorphous phase (a a process) was almost negligible, whereas the s's of the a c relaxation were an order of magnitude higher after recrystallization. Qualitatively, it was predicted by the soliton model for the a c relaxation. The recrystallization affected characteristics of the transition at the highest temperature (a) registered in the region of the melting of the crystals even more. The process is related to the relaxation of the space charge formed by ionogenic impurities, which migrate through the amorphous phase with high free volume. It was shown that the dynamics in the amorphous phase controlled the drift mobility of free charge carriers and, by that, determined the s of the space charge relaxation process. The structuring processes during the recrystallization also affected the parameters of the orderdisorder transition in the low-perfect ferroelectric (antiferroelectric) phase.

show abstract

Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts

Cited by 89 publications

References 31 publications

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

Cytotoxicity of commonly used luting cements —An <i>in vitro</i> study

In vitro potential cytotoxicity of an adhesive system to alveolar macrophages

Effect of recrystallization on the molecular mobility of a copolymer of vinylidene fluoride and hexafluoropropylene

Contact Info

Product

Resources

About