Pattern of Bone Generation after Irradiation in Vascularized Tissue Engineered Constructs

Eweida, Ahmad; Fathi, Ibrahim; Eltawila, Ahmed Maher; Elsherif, Ahmad M.; Elkerm, Yasser; Harhaus, Leila; Kneser, Ulrich; Sakr, Mahmoud

doi:10.1055/s-0037-1607322

Cited by 7 publications

(3 citation statements)

References 31 publications

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“…Several studies have demonstrated the utility of the axial vascularization concept in tissue-engineered constructs and its ability to promote vascularized tissue formation ( Eweida et al, 2018a ; Eweida et al, 2018b ; Wong et al, 2019 ). However, few studies have examined the suitability of axially vascularized chambers for islet transplantation in the form of an AV shunt ( Maki et al, 1996 ; Brown et al, 2006 ) or bundle ( Knight et al, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis

Fathi

Imura

Inagaki

et al. 2021

Front. Bioeng. Biotechnol.

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Introduction: Whole-organ decellularization is an attractive approach for three-dimensional (3D) organ engineering. However, progress with this approach is hindered by intra-vascular blood coagulation that occurs after in vivo implantation of the re-cellularized scaffold, resulting in a short-term graft survival. In this study, we explored an alternative approach for 3D organ engineering through an axial pre-vascularization approach and examined its suitability for pancreatic islet transplantation.Methods: Whole livers from male Lewis rats were decellularized through sequential arterial perfusion of detergents. The decellularized liver scaffold was implanted into Lewis rats, and an arteriovenous bundle was passed through the scaffold. At the time of implantation, fresh bone marrow preparation (BM; n = 3), adipose-derived stem cells (ADSCs; n = 4), or HBSS (n = 4) was injected into the scaffold through the portal vein. After 5 weeks, around 2,600 islet equivalents (IEQs) were injected through the portal vein of the scaffold. The recipient rats were rendered diabetic by the injection of 65 mg/kg STZ intravenously 1 week before islet transplantation and were followed up after transplantation by measuring the blood glucose and body weight for 30 days. Intravenous glucose tolerance test was performed in the cured animals, and samples were collected for immunohistochemical (IHC) analyses. Micro-computed tomography (CT) images were obtained from one rat in each group for representation.Results: Two rats in the BM group and one in the ADSC group showed normalization of blood glucose levels, while one rat from each group showed partial correction of blood glucose levels. In contrast, no rats were cured in the HBSS group. Micro-CT showed evidence of sprouting from the arteriovenous bundle inside the scaffold. IHC analyses showed insulin-positive cells in all three groups. The number of von-Willebrand factor-positive cells in the islet region was higher in the BM and ADSC groups than in the HBSS group. The number of 5-bromo-2′-deoxyuridine-positive cells was significantly lower in the BM group than in the other two groups.Conclusions: Despite the limited numbers, the study showed the promising potential of the pre-vascularized whole-organ scaffold as a novel approach for islet transplantation. Both BM- and ADSCs-seeded scaffolds were superior to the acellular scaffold.

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Section: Discussionmentioning

confidence: 99%

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis

Fathi

Imura

Inagaki

et al. 2021

Front. Bioeng. Biotechnol.

Self Cite

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“…Whether the nerve depends on capillary growth is a prerequisite theory for studying the nerve growth of bone tissue. It is currently believed that nerves play a common role in the nerves of bone tissue [14]. In the process of bone healing, there is a unique neurological aid, which occurs earlier than capillary growth, and the development of peptide nerves is closely related to capillary growth.…”

Section: Introductionmentioning

confidence: 99%

“…The various genes that transcribe these proteins often have incomprehensible genetic institutions. Gene display of nerves also occurs in cells or special tissues [20].…”

Section: Introductionmentioning

confidence: 99%

Experimental Examination of Vascularized Bone Tissue Based on Combined Mold Technology

2022

IJPHPM

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How to improve the bone healing speed and bone healing quality of large bone tissue is the current research hotspot. Capillary vascularization is the initial and most basic link, which has the decisive influence. If it is not possible to form an effective blood circulation, the manufacture of related bone tissue engineering materials can only be limited to a small range. Only by further exploring the status and method of action in the healing of bone tissue can we provide a useful method for solving their doubts. The purpose of this paper is to establish a research model for vascular nerve that can be used in bone tissue mechanisms, using composite mold technology to study vascularized bone tissue, observing the effects of sensory nerve bundles and motor nerve bundles on the growth and reconstruction of bone tissue. Whether the capillary bundle has a subtle influence on the development of bone tissue when the bone healed in bone tissue. To explore the role of vascular nerve in bone tissue construction, and to analyze the effects of different types of nerve fibers on bone healing. In this paper, by preparing vascular nerved bone tissue experiments, compared the influence of nerves and capillaries on bone healing in regenerated bone healing tissues from tissue immunology. And use immunological staining to initially detect the mechanism that promotes bone healing. Using the combination of mold technology and surgical methods, applying sensory nerves, motor nerves, and the combination of the two into the bone healing tissue, analyzed the influence and mechanism of nerves on bone healing in bone healing tissues. The results of the study show that bone tissue is originally accompanied by the growth of nerve fibers and the growth of nerve fibers is parallel to the process of bone healing. During the healing of bones in bone tissue, the sensory nerves or sensory nerves contact the motor nerves can promote bone healing. However, there is no obvious promotion effect when simply placed in the motor nerve, and the regenerated nerve fiber is very meaningful for the new capillaries in bone healing.

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Hypoxia as a stimulus for tissue formation: The concept of organogenesis in microsurgically vascularized tissue engineering constructs

Eweida,

Sandberg,

Ritthaler

et al. 2024

Journal of Cranio-Maxillofacial Surgery

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Pattern of Bone Generation after Irradiation in Vascularized Tissue Engineered Constructs

Abstract: The study shows for the first time the pattern of bone formation in AVTECs after irradiation using doses comparable to those applied after head and neck cancer. Axial vascularization probably enhances the osteoinductive properties in the central regions of AVTECs after irradiation.

Cited by 7 publications

References 31 publications

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis

Decellularized Whole-Organ Pre-vascularization: A Novel Approach for Organogenesis

Experimental Examination of Vascularized Bone Tissue Based on Combined Mold Technology

Hypoxia as a stimulus for tissue formation: The concept of organogenesis in microsurgically vascularized tissue engineering constructs

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