Pattern of Antimicrobial Susceptibility Obtained from Blood Isolates of a Rare but Emerging Human Pathogen, Gordonia polyisoprenivorans

Moser, Benjamin D.; Pellegrini, Gerald J.; Lasker, Brent A.; Brown, June M.

doi:10.1128/aac.01251-12

Cited by 23 publications

(20 citation statements)

References 7 publications

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“…In fact, two of the four patients in this study had broken Tenckhoff catheter tips 1 to 2 weeks prior to the development of peritonitis, which likely predisposed those patients to the entry of the Gordonia bacteria into the peritoneal cavities. Notably, Gordonia species have been most commonly reported to be causes of indwelling device-associated infections (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30), in line with these cases of Gordonia CAPD-related peritonitis.…”

Section: Fig 1 Continuedmentioning

confidence: 77%

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Lam

Leung³

et al. 2015

J Clin Microbiol

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We report here four cases of continuous ambulatory peritoneal dialysis-related peritonitis caused by three different species of Gordonia. The portal of entry was likely through Tenckhoff catheters. 16S rRNA and secA1 gene sequencing are so far the most reliable methods for the accurate identification of Gordonia species. Gordonia species are Gram-positive weakly acid-fast coryneform bacteria. Although Gordonia species have been implicated in a variety of infections, only seven cases of continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis caused by Gordonia species have been described (1-4). Furthermore, the methods used for identifying these seven Gordonia isolates were not mentioned in five cases (2, 4), and the accuracy of the identifications could not be ascertained. In this article, we described four cases of CAPD-related peritonitis caused by three different species of Gordonia confirmed by 16S rRNA and secA1 gene sequencing.Clinical specimens were collected and handled according to standard protocols, and the clinical data were collected by analyzing the patients' hospital records. Phenotypic identification was performed using standard conventional biochemical methods and the API Coryne system (bioMérieux, France). All tests were performed in triplicate. The MICs were determined using Etests (bioMérieux), according to the standards of the Clinical and Laboratory Standards Institute (CLSI) (5), with Escherichia coli strain ATCC 25922 and Pseudomonas aeruginosa strain ATCC 27853 as controls; the results were compared with the CLSI MIC interpretive standards for Staphylococcus spp. and Streptococcus pneumoniae (6) for evaluation. Bacterial DNA extraction, PCR amplification, and DNA sequencing of the 16S rRNA and secA1 genes were performed according to our previous publication (7), except for the primer pairs used (G268F/G1096R [8] for the 16S rRNA gene and SecA1-f/SecA1-r [9] for the secA1 gene). Comparative sequence identity analysis and phylogenetic analysis using the maximum likelihood method were performed according to our previous publication (10), except that MEGA 6.06 (11) was used instead. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was performed according to our previous publication (12), and the protein profiles obtained were processed and analyzed using MALDI Biotyper 3.1 (Bruker Daltonics, Germany) for the generation of the hierarchical cluster analysis (HCA) dendrogram and for identification. Case 1. A 65-year-old Chinese man was admitted for cloudy dialysis effluent for 1 day. He had end-stage renal failure (ESRF) of unknown etiology and had been undergoing CAPD for 7 years. He was afebrile but had turbid dialysis effluent. The total leukocyte count of the dialysis fluid was 930/mm 3 , with neutrophil predominance. A Gram smear of the dialysis effluent after centrifugation revealed numerous leukocytes without any organisms. Empirical intraperitoneal (i.p.) cefazolin and gentamicin therapies were started. As the clinical condition of...

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Section: Fig 1 Continuedmentioning

confidence: 77%

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Lam

Leung³

et al. 2015

J Clin Microbiol

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“…The levels of identity of AAC(3)-XI with the AAC(3)-VII, -VIII, -IX, and -X types were between 9.6 and 15.3%. The AAC(3)-XI enzyme was more closely related to the AAC(3)-I type (16 to 22% identity) and to the predicted annotated acetyltransferases found in environmental bacteria such as Salinispora tropica (53.2% identity, 36.1% similarity), Nocardioides (55.1% identity, 67.7% similarity), and Gordonia polyisoprenivorans (55.4% identity, 68.2% similarity), a rare but emerging human pathogen (27). Corynebacterium striatum, previously considered a saprophyte of skin and nasal mucosa, is increasingly associated with serious infections (1); the identification of AAC(3)-XI is therefore of clinical importance.…”

Section: Resultsmentioning

confidence: 98%

AAC(3)-XI, a New Aminoglycoside 3- N -Acetyltransferase from Corynebacterium striatum

Galimand

Fishovitz

Lambert

et al. 2015

Antimicrob Agents Chemother

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dCorynebacterium striatum BM4687 was resistant to gentamicin and tobramycin but susceptible to kanamycin A and amikacin, a phenotype distinct among Gram-positive bacteria. Analysis of the entire genome of this strain did not detect any genes for known aminoglycoside resistance enzymes. Yet, annotation of the coding sequences identified 12 putative acetyltransferases or GCN5-related N-acetyltransferases. A total of 11 of these coding sequences were also present in the genomes of other Corynebacterium spp. The 12th coding sequence had 55 to 60% amino acid identity with acetyltransferases in Actinomycetales. The gene was cloned in Escherichia coli, where it conferred resistance to aminoglycosides by acetylation. The protein was purified to homogeneity, and its steady-state kinetic parameters were determined for dibekacin and kanamycin B. The product of the turnover of dibekacin was purified, and its structure was elucidated by high-field nuclear magnetic resonance (NMR), indicating transfer of the acetyl group to the amine at the C-3 position. Due to the unique profile of the reaction, it was designated aminoglycoside 3-N-acetyltransferase type XI.

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“…It is one of the most effective rubber-degrading organisms known (19). Its ability to utilize degrading rubber as a sole carbon source, along with biosurfactant production to form biofilms for attachment, may explain the association of bacteremia in immunocompromised patients with chronic indwelling catheters (1,19,20). In addition to our case, four other cases of G. polyisoprenivorans bacteremia have been reported and all of the cases thus far have been associated with an indwelling central catheter.…”

Section: Case 4 (Gordonia Bronchialis)mentioning

confidence: 89%

“…The previously reported cases have been treated with a variety of antimicrobials for various periods of time. Recently, the in vitro antimicrobial susceptibilities of 13 human isolates of G. polyisoprenivorans were reported by Moser et al (20). Nearly half of the strains were resistant to trimethoprim-sulfamethoxazole.…”

Section: Case 4 (Gordonia Bronchialis)mentioning

confidence: 99%

“…Some strains were resistant to tigecycline, minocycline, and clarithromycin. All isolates were susceptible to amikacin, ampicillin, ceftriaxone, imipenem, amoxicillin-clavulanate, ciprofloxacin, vancomycin, and linezolid (20). Our isolates were tested in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines available at the time of isolate identification (38).…”

Section: Case 4 (Gordonia Bronchialis)mentioning

confidence: 99%

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Gordonia Bacteremia

2013

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Pattern of Antimicrobial Susceptibility Obtained from Blood Isolates of a Rare but Emerging Human Pathogen, Gordonia polyisoprenivorans

Cited by 23 publications

References 7 publications

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

Gordonia Species as Emerging Causes of Continuous-Ambulatory-Peritoneal-Dialysis-Related Peritonitis Identified by 16S rRNA and secA1 Gene Sequencing and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS)

AAC(3)-XI, a New Aminoglycoside 3- N -Acetyltransferase from Corynebacterium striatum

Gordonia Bacteremia

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