Pattern formation during vasculogenesis

Czirók, András; Little, Charles D.

doi:10.1002/bdrc.21010

Cited by 24 publications

(23 citation statements)

References 97 publications

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“…Additionally, to further probe the mechanisms underlying the observations found in this study, it would be useful to pair these experiments with a computational modeling approach. [42][43][44] In closing, this study provides a significant contribution to the effort of quantifying the science of microvascular tissue engineering and creating MVNs with prescribed morphological properties for tissue-specific applications.…”

mentioning

confidence: 90%

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Whisler

Chen

Kamm

2014

Tissue Engineering Part C: Methods

203

233

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The mechanical and biochemical microenvironment influences the morphological characteristics of microvascular networks (MVNs) formed by endothelial cells (ECs) undergoing the process of vasculogenesis. The objective of this study was to quantify the role of individual factors in determining key network parameters in an effort to construct a set of design principles for engineering vascular networks with prescribed morphologies. To achieve this goal, we developed a multiculture microfluidic platform enabling precise control over paracrine signaling, cell-seeding densities, and hydrogel mechanical properties. Human umbilical vein endothelial cells (HUVECs) were seeded in fibrin gels and cultured alongside human lung fibroblasts (HLFs). The engineered vessels formed in our device contained patent, perfusable lumens. Communication between the two cell types was found to be critical in avoiding network regression and maintaining stable morphology beyond 4 days. The number of branches, average branch length, percent vascularized area, and average vessel diameter were found to depend uniquely on several input parameters. Importantly, multiple inputs were found to control any given output network parameter. For example, the vessel diameter can be decreased either by applying angiogenic growth factors-vascular endothelial growth factor (VEGF) and sphingosine-1-phsophate (S1P)-or by increasing the fibrinogen concentration in the hydrogel. These findings introduce control into the design of MVNs with specified morphological properties for tissue-specific engineering applications.

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mentioning

confidence: 90%

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Whisler

Chen

Kamm

2014

Tissue Engineering Part C: Methods

203

233

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“…90 In their 35 modified Cellular Potts Model cells prefer to be adjacent to other stalk cells rather than staying in the aggregate (see Reference video 13). The presence of persistently moving tip cells and the preferential adhesion assumption are together sufficient to generate expanding sprouts in computer simulations with this 40 model (reviewed in: 91,92,16 ).…”

Section: Modeling Of Vasculogenesis By Biophysical Mechanismmentioning

confidence: 99%

Collective motion of cells: from experiments to models

2014

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Swarming or collective motion of living entities is one of the most common and spectacular manifestations of living systems that have been extensively studied in recent years. A number of general principles have been established. The interactions at the level of cells are quite different from those among individual animals, therefore the study of collective motion of cells is likely to reveal some specific important features which we plan to overview in this paper. In addition to presenting the most appealing results from the quickly growing related literature we also deliver a critical discussion of the emerging picture and summarize our present understanding of collective motion at the cellular level. Collective motion of cells plays an essential role in a number of experimental and real-life situations. In most cases the coordinated motion is a helpful aspect of the given phenomenon and results in making a related process more efficient (e.g., embryogenesis or wound healing), while in the case of tumor cell invasion it appears to speed up the progression of the disease. In these mechanisms cells both have to be motile and adhere to one another, the adherence feature being the most specific to this sort of collective behavior. One of the central aims of this review is to present the related experimental observations and treat them in light of a few basic computational models so as to make an interpretation of the phenomena at a quantitative level as well.

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“…It is worth noting that the morphological features of the mouse vascular pattern (in vitro) and primary quail vascular patterns (in vivo) are essentially the same. Thus, although it is necessary to identify and understand the requisite molecular and genetic regulatory mechanisms, it is equally important to understand the dynamic biophysical mechanisms required for patterning a new vascular bed (Czirok and Little, 2012;Gamba et al, 2003;Merks et al, 2006Merks et al, , 2008Serini et al, 2003;van Oers et al, 2014). It is noteworthy that (B) Driven by tissue deformation of cardiac progenitors and ECM, the right and left heart primordia fuse at the midline -the cardinal step in forming the tube-within-atube morphology of the amniote heart.…”

Section: Primary Vasculogenesismentioning

confidence: 99%

Extracellular matrix motion and early morphogenesis

et al. 2016

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For over a century, embryologists who studied cellular motion in early amniotes generally assumed that morphogenetic movement reflected migration relative to a static extracellular matrix (ECM) scaffold. However, as we discuss in this Review, recent investigations reveal that the ECM is also moving during morphogenesis. Time-lapse studies show how convective tissue displacement patterns, as visualized by ECM markers, contribute to morphogenesis and organogenesis. Computational image analysis distinguishes between cell-autonomous (active) displacements and convection caused by large-scale (composite) tissue movements. Modern quantification of large-scale 'total' cellular motion and the accompanying ECM motion in the embryo demonstrates that a dynamic ECM is required for generation of the emergent motion patterns that drive amniote morphogenesis.

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Pattern formation during vasculogenesis

Cited by 24 publications

References 97 publications

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Collective motion of cells: from experiments to models

Extracellular matrix motion and early morphogenesis

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