Pattern‐deficient forelimb regeneration in adult bullfrogs

Tomlinson, Bruce L.; Tomlinson, Donna E.; Tassava, Roy A.

doi:10.1002/jez.1402360309

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…5 anterior (19). This is consistent with an earlier notion that the breaking of symmetrical assembly of a photoreceptor cluster into asymmetry occurs as R4 loses its contact with R8 resulting in the breaking of R3-R4 symmetry (26). It was also suggested that the sple function is required in R3, R4, or R5 for correct polarity of a photoreceptor cluster (19).…”

Section: Resultssupporting

confidence: 91%

Independent determination of symmetry and polarity in the Drosophila eye.

Choi

Mozer

Benzer

1996

Proc. Natl. Acad. Sci. U.S.A.

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In each facet ofthe Drosophila compound eye, a cluster of photoreceptor cells assumes an asymmetric trapezoidal pattern. These (11,12) as well as ectopic equators (5). These studies suggest that the direction of furrow progression plays an important role in controlling both ommatidial polarity and equator. However, it is unclear whether ommatidial polarity and more global determinants are determined by the same mechanisms.Morphogenetic processes involved in cuticular polarity formation have been studied using strains referred to as "polarity" mutants that have altered orientations of hairs and bristles (13). Interestingly, some polarity mutants, including spiny legs (sple) (14, 15), dishevelled (dsh) (16), and frizzled (fz) (17, 18), also show abnormal polarity of the photoreceptor clusters, providing some clues to study genetic mechanisms underlying the ommatidial polarity formation.Among several polarity mutants, sple shows remarkably specific pattern defects: the majority of abnormal ommatidia in the sple eye are only dorsoventrally reversed and show complete 90°r otations, whereas fz shows a mixture of eye phenotypes such as D/V and A/P reversals and incomplete rotation (19). We have isolated a P-lacW enhancer trap strain in which w+ gene expression is restricted to the dorsal domain providing a useful D/V marker. We here address the relationship between the global mirror symmetry and the local ommatidial polarity, using the mutant sple and the P-lacW enhancer trap marker. MATERIALS AND METHODSFly Stocks. Bl-12 was isolated from an enhancer trap screen (unpublished results) using the procedure described in Bier et al. (4). Bl-12 contains a single P-lacW insertion at the 69 D region of the third chromosome (data not shown). Homozygosity for Bl-12 causes lethality. Occasionally (less than 1%),

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Section: Resultssupporting

confidence: 91%

Independent determination of symmetry and polarity in the Drosophila eye.

Choi

Mozer

Benzer

1996

Proc. Natl. Acad. Sci. U.S.A.

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“…Wings were dissected and mounted in 50% ethanol, 50% lactic acid. Sections of adult eyes were prepared as described in (Tomlinson, 1985).…”

Section: Histochemical and Microscopic Analysismentioning

confidence: 99%

A Drosophila analogue of v-Cbl is a dominant-negative oncoprotein in vivo

et al. 2000

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“…Tissue regeneration occurs in many different animal species, with the capacity for regeneration varying significantly among even highly related animals (Alibardi, 2017; Brockes & Gates, 2014; Dwaraka & Voss, 2021; Goss & Holt, 1992; McLaughlin et al, 1983; Nogueira et al, 2016; Simon & Tanaka, 2013; Tomlinson et al, 1985). Not only do these different vertebrate species share an ability to regenerate but there is an overlap in the molecular pathways involved in regeneration between the early blastema of ray‐finned fish and lobe‐finned vertebrates (Darnet et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Suppressors of cGAS‐STING are downregulated during fin‐limb regeneration and aging in aquatic vertebrates

Mathavarajah,

Thompson,

Stoyek

et al. 2023

J Exp Zool Pt B

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During the early stages of limb and fin regeneration in aquatic vertebrates (i.e., fishes and amphibians), blastema undergo transcriptional rewiring of innate immune signaling pathways to promote immune cell recruitment. In mammals, a fundamental component of innate immune signaling is the cytosolic DNA sensing pathway, cGAS‐STING. However, to what extent the cGAS‐STING pathway influences regeneration in aquatic anamniotes is unknown. In jawed vertebrates, negative regulation of cGAS‐STING activity is accomplished by suppressors of cytosolic DNA such as Trex1, Pml, and PML‐like exon 9 (Plex9) exonucleases. Here, we examine the expression of these suppressors of cGAS‐STING, as well as inflammatory genes and cGAS activity during caudal fin and limb regeneration using the spotted gar (Lepisosteus oculatus) and axolotl (Ambystoma mexicanum) model species, and during age‐related senescence in zebrafish (Danio rerio). In the regenerative blastema of wounded gar and axolotl, we observe increased inflammatory gene expression, including interferon genes and interleukins 6 and 8. We also observed a decrease in axolotl Trex1 and gar pml expression during the early phases of wound healing which correlates with a dramatic increase in cGAS activity. In contrast, the plex9.1 gene does not change in expression during wound healing in gar. However, we observed decreased expression of plex9.1 in the senescing cardiac tissue of aged zebrafish, where 2′3′‐cGAMP levels are elevated. Finally, we demonstrate a similar pattern of Trex1, pml, and plex9.1 gene regulation across species in response to exogenous 2′3′‐cGAMP. Thus, during the early stages of limb‐fin regeneration, Pml, Trex1, and Plex9.1 exonucleases are downregulated, presumably to allow an evolutionarily ancient cGAS‐STING activity to promote inflammation and the recruitment of immune cells.

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Pattern‐deficient forelimb regeneration in adult bullfrogs

Cited by 7 publications

References 30 publications

Independent determination of symmetry and polarity in the Drosophila eye.

Independent determination of symmetry and polarity in the Drosophila eye.

A Drosophila analogue of v-Cbl is a dominant-negative oncoprotein in vivo

Suppressors of cGAS‐STING are downregulated during fin‐limb regeneration and aging in aquatic vertebrates

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