Patients with colorectal cancer have identical strains of <i>Fusobacterium nucleatum</i> in their colorectal cancer and oral cavity

Komiya, Yasuhiko; Shimomura, Yumi; Higurashi, Takuma; Sugi, Yutaka; Arimoto, Jun; Umezawa, Shotaro; Uchiyama, S.; Matsumoto, Mitsuharu; Nakajima, Atsushi

doi:10.1136/gutjnl-2018-316661

Cited by 212 publications

(204 citation statements)

References 9 publications

Supporting

Mentioning

189

Contrasting

Unclassified

Order By: Relevance

“…37 The presence of F. nucleatum was validated after sequencing using quantitative polymerase chain reaction (qPCR) to detect its specific gene markers. 37 With F. nucleatum-specific primer sets, it was confirmed that F. nucleatum detected in the CRC samples showed a high similarity with that detected in the saliva, 39 indicating the possible oral origin of F. nucleatum in CRC samples. With the help of fluorescence in-situ hybridization technique, F. nucleatum-specific fluorescent probe was used to reveal the significant abundance of F. nucleatum in colorectal tumors.…”

Section: Fusobacterium Nucleatummentioning

confidence: 77%

Role of the oral microbiota in cancer evolution and progression

Sun

Tang

et al. 2020

Cancer Medicine

View full text Add to dashboard Cite

Bacteria identified in the oral cavity are highly complicated. They include approximately 1000 species with a diverse variety of commensal microbes that play crucial roles in the health status of individuals. Epidemiological studies related to molecular pathology have revealed that there is a close relationship between oral microbiota and tumor occurrence. Oral microbiota has attracted considerable attention for its role in in‐situ or distant tumor progression. Anaerobic oral bacteria with potential pathogenic abilities, especially Fusobacterium nucleatum and Porphyromonas gingivalis, are well studied and have close relationships with various types of carcinomas. Some aerobic bacteria such as Parvimonas are also linked to tumorigenesis. Moreover, human papillomavirus, oral fungi, and parasites are closely associated with oropharyngeal carcinoma. Microbial dysbiosis, colonization, and translocation of oral microbiota are necessary for implementation of carcinogenic functions. Various underlying mechanisms of oral microbiota‐induced carcinogenesis have been reported including excessive inflammatory reaction, immunosuppression of host, promotion of malignant transformation, antiapoptotic activity, and secretion of carcinogens. In this review, we have systemically described the impact of oral microbial abnormalities on carcinogenesis and the future directions in this field for bringing in new ideas for effective prevention of tumors.

show abstract

Section: Fusobacterium Nucleatummentioning

confidence: 77%

Role of the oral microbiota in cancer evolution and progression

Sun

Tang

et al. 2020

Cancer Medicine

View full text Add to dashboard Cite

show abstract

“…https://doi.org /10.1101/2020.01.30.20019752 doi: medRxiv preprint Actinomyces, Haemophilus, Rothia, Streptococcus, and Veilonella 24 . A letter to the editor reported that Fusobacteruim nucleatum was detected in 8 (57.1%) of 14 CRC patients' tumor and saliva samples, and identical strains were found in 75% of the tumor and saliva samples suggesting that F. nucleatum in colorectal tumor originates in the oral cavity 25 . In the present study, we identified 73 ASVs shared between oral and intestinal or pancreatic (Figure 1; Supplemental table 1).…”

Section: Discussionmentioning

confidence: 99%

Oral, intestinal, and pancreatic microbiomes are correlated and exhibit co-abundance in patients with pancreatic cancer and other gastrointestinal diseases

Chung

Zhao

Meier

et al. 2020

Preprint

View full text Add to dashboard Cite

Oral microbiota are believed to play important roles in systemic diseases, including cancer. We collected oral swabs and at least one pancreatic tissue or intestinal samples from 52 subjects, and characterized 16S ribosomal RNA genes using high-throughput DNA sequencing in a total 324 samples. We identified a total of 73 unique Amplicon Sequence Variants (ASVs) that were shared between oral and pancreatic or intestinal samples. Accounting for pairing and within-subject correlation, 7 ASVs showed significant concordance (Kappa statistics) and 5 ASVs exhibited significant or marginally significant Pairwise Stratified Association (PASTA) between oral samples and pancreatic tissue or intestinal samples. Of these, two specific bacterial species (Gemella morbillorum and Fusobacterium nucleatum subsp. vincentii) showed consistent presence or absence patterns between oral and intestinal or pancreatic samples. Lastly, our microbial co-abundance analyses showed several distinct ASVs clusters and complex correlation-networks between ASV clusters in buccal, saliva, duodenum, jejunum, and pancreatic tumor samples. Toghether, our findings suggest that oral, intestinal, and pancreatic microbiomes are correlated and bacteria of oral origin exhibit co-abundance relationships and demonstrate complex correlation patterns in the intestinal and pancreatic tumor samples. Future prospective studies should aim to uncover the co-abundance of specific microbial communities for studying etiology of microbiota-driven carcinogenesis.

show abstract

“…The oral cavity as reservoir. It has been shown that in a number of cases (6/14, 43%) identical F. nucleatum strains could be recovered from CRC and saliva of the same patients [Komiya et al 2019]. Furthermore, the oral microbiome composition is to a certain extent predictive for CRC disease progress status [Flemer et al 2018].…”

Section: Cohort Specific Speciesmentioning

confidence: 99%

The Presence of Periodontal Pathogens in Gastric Cancer

Leeuw¹,

Duval²

2020

Preprint

View full text Add to dashboard Cite

BackgroundThe microbiome is thought to play a role in the development of gastric cancer (GC). Several studies have put forward putatively carcinogenic species in addition to Helicobacter pylori, but are not in perfect alignment, possibly due to variable parameters in the experiments, including downstream processing. Meta-analyses have not been published so far, so there is lack in clinical guidance beyond H. pylori eradication therapy.MethodsHere, we analysed gastric mucosa samples from nine public data sets, including GC samples. Using both unsupervised and supervised learning, we defined fine grain bacterial networks of gastric mucosa and identified species associated with tumor status of samples.ResultsWe found anatomic locations and cohort regions among the possible factors leading to the observation of study specific gastric microbiomes. Despite this variability, the periodontal species Fusobacterium nucleatum, Parvimonas micra and Peptostreptococcus stomatis were found in association with tumor status in several datasets. The three species were predicted to be in interaction by ecological network analysis and also formed the intersection of tumor associated species between four GC data sets and five colorectal cancer (CRC) data sets we reanalyzed. We formulated a probiotic composition putatively competing with the GC pathogen spectrum, from correlation analysis in a large superset of gut samples (n=17,800) from clinical- and crowd-sourced studies.ImplicationsThe overlapping bacterial pathogen spectrum between two gastrointestinal tumor types, GC and CRC, has implications for etiology, treatment and prevention. In vitro testing results reported in literature suggest H. pylori eradication treatment should be efficient against the GC pathogen spectrum, yet the existence of an upstream periodontal reservoir is of concern. To address this, we propose longer term use of the formulated probiotics composition.

show abstract

Patients with colorectal cancer have identical strains of Fusobacterium nucleatum in their colorectal cancer and oral cavity

Cited by 212 publications

References 9 publications

Role of the oral microbiota in cancer evolution and progression

Role of the oral microbiota in cancer evolution and progression

Oral, intestinal, and pancreatic microbiomes are correlated and exhibit co-abundance in patients with pancreatic cancer and other gastrointestinal diseases

The Presence of Periodontal Pathogens in Gastric Cancer

Contact Info

Product

Resources

About