“Patient‐Like” nude mouse metastatic model of advanced human pleural cancer

Abstract

Pleural cancer in humans is a frequently occurring tumor. Recently, clinical trials have suggested that chemotherapy and immunotherapy administered intrapleurally may elicit responses in early-stage diseases. However, at radiological and pleural endoscopic evaluation, most of the patients are found to have a visceral pleural involvement that is generally refractory to therapy and leads to a poor prognosis. The goal of this study was to construct a nude mouse model of human parietal- and visceral-pleural cancer that could reflect the clinical picture for this disease. The model could then be useful for drug discovery for pleural cancer. A well-differentiated human lung adenocarcinoma was used as intact tissue for implantation. Ten mice underwent parietal-pleural implantation and ten mice visceral-pleural implantation via a novel thoracotomy procedure we have developed. Symptoms of tumor growth were determined from weight loss, respiratory distress, or debilitation. Actual tumor growth and spread were measured at autopsy. The mouse survival curves of each group were estimated by the Kaplan-Meier method and the difference of the median survival times was assessed by the Log-rank test. The slopes of mean-mouse weight curves were compared using a standard two-sample t-test. A 100% take rate was achieved in constructing the pleural cancer models. Tumor growth was initially assessed by symptomatology and survival: the median survival time was, respectively, 27.9 days and 31 days for visceral-pleural and parietal-pleural implanted groups (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

The dielectric properties of cancerous tissues in a nude mouse xenograft model

“…Surgical heterotransplantation of tumor tissue has been successfully established in xenograft models from a number of human sources [Fu et al, 1991;Astoul et al, 1994;Colt et al, 1996]. The advantage of human tissue heterotransplantation would be to transfer cancer cells with their native stroma and extracellular matrix.…”

Liver colonization competence governs colon cancer metastasis.

“…These models are the first in which animal-implanted human tumors closely replicate the complex behaviors of the original tumor while still in the human host. The implanted tumors parallel the original tumor's characteristic growth and local invasiveness, its drug sensitivity and, most important, the tumor's rate of metastases to corresponding target tissues (17)(18)(19)(20), pancreatic cancer (21,22), bladder cancer (23,24) lung cancer (25)(26)(27)(28)(29)(30)(31)(32)(33), ovarian cancer (34), breast cancer (35), and prostate cancer (36) Table 1. All strains were maintained by serial transplantation into nude mice (39,40).…”

Near Infrared Photoimmunotherapy in a Transgenic Mouse Model of Spontaneous Epidermal Growth Factor Receptor (EGFR)-expressing Lung Cancer

“…Another way to overcome the problem of the xenograft tumor model which does not fully represent human cancers is using orthotopic mouse models which can lead to metastasis mimicking to that seen in patients (11,24–28). However, surgical orthotopic implant requires highly trained surgical skills.…”