Patient factors affecting culture of Helicobacter pylori isolated from gastric mucosal specimens

Leszczyńska, Katarzyna; Namiot, Andrzej; Namiot, Z; Leszczyńska, JK; Jakoniuk, P; Chilewicz, M; Namiot, DB; Kemonä, A; Milewski, Robert; Bucki, Robert

doi:10.2478/v10039-010-0028-1

Cited by 17 publications

(9 citation statements)

References 13 publications

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“…It is possible that the group of noninfected children with gastritis did in fact coincidently include some H. pyloriinfected subjects. This fact may result from 1, the false negative results in the tests used for the H. pylori diagnosis, because of low bacterial load found in children [20] and 2, undetected previous H. pylori infection in some patients as the serologic tests for H. pylori infection were not performed. Additionally, gastritis itself (without a recognizable H. pylori infection) accounts for the lymphocyte activation, but because of the lack of additional stimulation by H. pylori, a relatively lower proportion of lymphocytes enter apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Pediatric Helicobacter pylori Infection and Circulating T-Lymphocyte Activation and Differentiation

et al. 2011

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In H. pylori-negative children, gastritis was associated with an increased percentage of activated NK and T cells, and intermediate-differentiated peripheral blood CD4(+) T cells, which was more pronounced in H. pylori-positive children who also showed an increased B-cell response. However, increased inflammation was only associated with the elevation of CD4(+) T-cell percentage in H. pylori-positive children as well as B-cell percentage in H. pylori-negative children with gastritis.

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Section: Discussionmentioning

confidence: 99%

Pediatric Helicobacter pylori Infection and Circulating T-Lymphocyte Activation and Differentiation

et al. 2011

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“…To avoid negative results due to the uneven distribution of H. pylori in the stomach and to increase the sensitivity and specificity of the method in the diagnosis of H. pylori, it is necessary to take several biopsies from the gastric mucosa: two from the antrum and two from the body of the stomach. Some authors believe that in order to increase the sensitivity and specificity of the bacteriological method, taking biopsies for cultivation should be carried out 3 months after patients cease taking PPIs, antibiotics, and bismuth [27].…”

Section: Culturementioning

confidence: 99%

Current Helicobacter pylori Diagnostics

et al. 2021

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The high prevalence of Helicobacter pylori and the variety of gastroduodenal diseases caused by this pathogen necessitate the use of only accurate methods both for the primary diagnosis and for monitoring the eradication effectiveness. There is a broad spectrum of diagnostic methods available for detecting H. pylori. All methods can be classified as invasive or non-invasive. The need for upper endoscopy, different clinical circumstances, sensitivity and specificity, and accessibility defines the method chosen. This article reviews the advantages and disadvantages of the current options and novel developments in diagnostic tests for H. pylori detection. The progress in endoscopic modalities has made it possible not only to diagnose precancerous lesions and early gastric cancer but also to predict H. pylori infection in real time. The contribution of novel endoscopic evaluation technologies in the diagnosis of H. pylori such as visual endoscopy using blue laser imaging (BLI), linked color imaging (LCI), and magnifying endoscopy is discussed. Recent studies have demonstrated the capability of artificial intelligence to predict H. pylori status based on endoscopic images. Non-invasive diagnostic tests such as the urea breathing test and stool antigen test are recommended for primary diagnosis of H. pylori infection. Serology can be used for initial screening and epidemiological studies. The histology showed its value in detecting H. pylori and provided more information about the degree of gastric mucosa inflammation and precancerous lesions. Molecular methods are mainly used in detecting antibiotic resistance of H. pylori. Cultures from gastric biopsies are the gold standard and recommended for antibiotic susceptibility tests.

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“…Several factors have been suggested to affect the successful culture of H pylori , including bacterial load, degree of gastritis, transport media, transport time, and temperature 11 . Some investigators suggested that rapid transport of biopsy specimens in an appropriate transport medium at low temperature is essential for a satisfactory recovery of H pylori 3,12 .…”

Section: Discussionmentioning

confidence: 99%

Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

et al. 2020

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Background and Aims Isolation of Helicobacter pylori is considered difficult because of the requirement of the additional biopsy tissue and the effort involved in the isolation of the bacterium. We investigated whether H pylori can be cultured from tissue samples used for the rapid urease test (RUT). Methods Totally, 174 specimens from 87 patients referred for endoscopy were prospectively included. During endoscopy, two biopsy specimens were obtained, one each from the gastric antrum and the corpus, and were placed into a commercially available RUT kit. After detection of urease activity, H pylori was cultured using tissue leftover in the RUT, regardless of the result. Results H pylori was successfully isolated using leftover tissue in 72.4% (63/87) of the patients. In 32 patients, H pylori was isolated from both specimens, while in 31 patients, it was isolated from either antrum or corpus. Eighty‐one H pylori strains were isolated from 141 specimens with positive RUT results (57.4%), whereas 14 strains were isolated from 33 specimens with negative RUT results (42.4%). The median interval between tissue acquisition and inoculation onto the isolation media was 3.6 hours (range: 0.5‐27.5 hours) in cases with successful cultures, compared to 23.5 hours (range: 0.5‐76.0 hours) in cases with failed cultures. Among the positive RUT tissues, 80.4% (45/56) were cultured successfully when the tissue was inoculated within 4 hours of the biopsy. Conclusions RUT kits can be used as transport media for H pylori, and this media is most efficient when used within 4 hours of the test.

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Patient factors affecting culture of Helicobacter pylori isolated from gastric mucosal specimens

Cited by 17 publications

References 13 publications

Pediatric Helicobacter pylori Infection and Circulating T-Lymphocyte Activation and Differentiation

Pediatric Helicobacter pylori Infection and Circulating T-Lymphocyte Activation and Differentiation

Current Helicobacter pylori Diagnostics

Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

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