2021
DOI: 10.3389/fphar.2021.679741
|View full text |Cite
|
Sign up to set email alerts
|

Patient Derived Colonoids as Drug Testing Platforms–Critical Importance of Oxygen Concentration

Abstract: Treatment of inflammatory bowel disease (IBD) is challenging, with a series of available drugs each helping only a fraction of patients. Patients may face time-consuming drug trials while the disease is active, thus there is an unmet need for biomarkers and assays to predict drug effect. It is well known that the intestinal epithelium is an important factor in disease pathogenesis, exhibiting physical, biochemical and immunologic driven barrier dysfunctions. One promising test system to study effects of existi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

4
21
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
4
1

Relationship

2
3

Authors

Journals

citations
Cited by 9 publications
(25 citation statements)
references
References 50 publications
4
21
0
Order By: Relevance
“…Beyond ATF3, no gene expression differences were verified on a protein level, and further verification would be required in follow-up studies of our initial observations. Indeed, we have previously published several studies where the initial hypotheses were formed on basis of the data presented in full for the first time here, thus representing a thorough validation of parts of the data [14,25,26].…”
Section: Plos Onementioning
confidence: 99%
“…Beyond ATF3, no gene expression differences were verified on a protein level, and further verification would be required in follow-up studies of our initial observations. Indeed, we have previously published several studies where the initial hypotheses were formed on basis of the data presented in full for the first time here, thus representing a thorough validation of parts of the data [14,25,26].…”
Section: Plos Onementioning
confidence: 99%
“…Pinch biopsies from patients were collected in 10% buffered formalin and histopathology was evaluated as described before ( 44 ). Colonoids for functional assays were established using pinch biopsies collected from uninflamed mucosa at the hepatic flexure ( 28 , 43 ). Patient characteristics are listed in Table 1 .…”
Section: Methodsmentioning
confidence: 99%
“…Colonoid cultures were established and grown in 20% O 2 and 5% CO 2 at 37°C as described in Skovdahl et al. ( 43 ), based on protocols of Mahe et al ( 40 ), and Jung et al. ( 45 ).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations