Pathways of hydrogen photoproduction by immobilized Chlamydomonas reinhardtii cells deprived of sulfur

Antal, Taras K.; Matorin, D. N.; Kukarskikh, G. P.; Lambreva, Maya D.; Tyystjärvi, Esa; Krendeleva, T. E.; Tsygankov, Anatoly A.; Rubin, A. B.

doi:10.1016/j.ijhydene.2014.08.135

Cited by 24 publications

(8 citation statements)

References 31 publications

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“…Different approaches have been proposed to increase the hydrogen production capacity in green algae (recently reviewed in Esquível et al 2011;Eroglu and Melis 2011;Antal et al 2011;Torzillo et al 2014;Dubini and Ghirardi 2014;Ghirardi 2015). In S-deprived C. reinhardtii, advances have been achieved by 1. optimization of cultivation parameters, such as Chl concentration, medium composition and pH, light intensity, CO 2 supply, and synchronization of cell division (Tsygankov et al 2002;Kosourov et al 2003;Laurinavichene et al 2004;Kim et al 2006;Giannelli et al 2009); 2. construction of photobioreactors and elaboration of special protocols for continuous and semi-continuous regimes of cultivation, including outdoor and largescale cultivations (Fedorov et al 2005;Oncel and Vardar-Sukan 2009;Scoma et al 2012); 3. immobilization/encapsulation of algal cells in alginate, agar, or other materials (Laurinavichene et al 2008;Kosourov and Seibert 2009;Rashid et al 2013;Antal et al 2014;Stojkovica et al 2015); 4. design of high hydrogen-producing mutants characterized by an elevated respiration rate, low CET, low trans-thylakoid proton gradient, modified starch metabolism, truncated light-harvesting antennae, more oxygen-tolerant H 2 ase, and screening of natural species (reviewed in Torzillo et al 2014;Dubini and Ghirardi 2014).…”

Section: Advances and Perspectives Of Hydrogen Photoproduction By Grementioning

confidence: 99%

“…Moreover, immobilization has proven to significantly increase the efficiency of conversion of light energy to hydrogen energy, compared with a suspension culture (Kosourov and Seibert 2009). The mechanisms of hydrogen photoproduction by immobilized, S-deprived C. reinhardtii were recently studied by Antal et al (2014). Results of the study indicate more rapid PSII inactivation, elevated contribution of a PSII-independent pathway of hydrogen photoproduction, and altered starch metabolism in immobilized cells.…”

Section: Photosynth Resmentioning

confidence: 99%

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Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production

2015

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A complex regulatory network in the chloroplast of green algae provides an efficient tool for maintenance of energy and redox balance in the cell under aerobic and anaerobic conditions. In this review, we discuss the structural and functional organizations of electron transport pathways in the chloroplast, and regulation of photosynthesis in the green microalga Chlamydomonas reinhardtii. The focus is on the regulatory mechanisms induced in response to nutrient deficiency stress and anoxia and especially on the role of a hydrogenase-mediated reaction in adaptation to highly reducing conditions and ATP deficiency in the cell.

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Section: Advances and Perspectives Of Hydrogen Photoproduction By Grementioning

confidence: 99%

Section: Photosynth Resmentioning

confidence: 99%

Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production

2015

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“…It is well established that the immobilization of S‐deprived cells may alter important characteristics including the photosynthetic capacity and H 2 photoproduction (Kosourov and Seibert , Antal et al ). Thus, H 2 photoproduction period increases from 3 to 4 days in suspension S‐deprived cultures up to 3 weeks in immobilized cells.…”

Section: Resultsmentioning

confidence: 99%

Comparative analyses of H₂ photoproduction in magnesium‐ and sulfur‐starved Chlamydomonas reinhardtii cultures

Волгушева

Jokel

Allahverdiyeva

et al. 2017

Physiologia Plantarum

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Magnesium (Mg)-deprived Chlamydomonas reinhardtii cells are capable to sustain hydrogen (H ) photoproduction at relatively high photosystem II (PSII) activity levels for an extended time period as compared with sulfur (S)-deprived cells. Herein, we present a comparative study of H photoproduction induced by Mg and S shortage to unravel the specific rearrangements of the photosynthetic machinery and cell metabolism occurring under the two deprivation protocols. The exhaustive analysis of photosynthetic activity and regulatory pathways, respiration and starch metabolism revealed the specific rearrangements of the photosynthetic machinery and cellular metabolism, which occur under the two deprivation conditions. The obtained results allowed us to conclude that the expanded time period of H production upon Mg-deprivation is due to the less harmful effects that Mg-depletion has on viability and metabolic performance of the cells. Unlike S-deprivation, the photosynthetic light and dark reactions in Mg-deprived cells remained active over the whole H production period. However, the elevated PSII activity in Mg-deprived cells was counteracted by the operation of pathways for O consumption that maintain anaerobic conditions in the presence of active water splitting.

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“…However, several systems have been described which utilise immobilised algal cells (e.g. by Antal et al [100]). The possibility of selectively controlling algal interactions with scaffolds means that in future, bioreactors may hold algal populations in defined 3D configurations that enable the organism to adapt its biology to its individual situation, while also providing the possibility of controlled gas and nutrient exchange through the scaffold material which could, for example, obviate the problem of oxygen generation during photobiological hydrogen production.…”

Section: Scaffolding Behaviourmentioning

confidence: 99%

Prospects for Photobiological Hydrogen as a Renewable Energy

Ross

Oey²,

Stephens³

et al. 2016

CBIOT

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BACKGROUND: Hydrogen is a clean, versatile fuel and energy carrier which can be produced by a range of renewable technologies for combustion, use in fuel cells, or as a manufacturing feedstock. Despite its attraction and significant technological innovation, commercial feasibility of photobiological hydrogen processes is far from demonstrated. OBJECTIVE: This review examines direct photobiological biohydrogen systems, with a particular focus on the main obstacles that must be overcome to deliver commercially viable, net energy positive systems. As part of this process the interactions between future photobiological biohydrogen systems and other parts of a renewable energy economy are examined to analyse potential technology integration paths. RESULTS: The primary driver for renewably produced hydrogen is the potential for CO 2 emissions reductions. Renewable hydrogen is largely solar driven, either directly (e.g. natural photosynthesis, or bio-inspired devices) or indirectly (e.g. fermentation, electrical hydrolysis). A significant market for hydrogen already exists and is supported by extensive infrastructure providing significant opportunities for emerging renewable hydrogen streams. Several key physiological obstacles to efficient photobiohydrogen production have already been overcome, with oxygen tolerance as the most significant remaining problem. CONCLUSIONS: A much deeper understanding of photosynthetic biology is required before existing knowledge can be integrated with real world systems. Cross-fertilisation between engineering and biology represents the best path forward for implementation as a robust biotechnology.

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Pathways of hydrogen photoproduction by immobilized Chlamydomonas reinhardtii cells deprived of sulfur

Cited by 24 publications

References 31 publications

Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production

Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production

Comparative analyses of H₂ photoproduction in magnesium‐ and sulfur‐starved Chlamydomonas reinhardtii cultures

Prospects for Photobiological Hydrogen as a Renewable Energy

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Pathways of hydrogen photoproduction by immobilized Chlamydomonas reinhardtii cells deprived of sulfur

Cited by 24 publications

References 31 publications

Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production

Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production

Comparative analyses of H2 photoproduction in magnesium‐ and sulfur‐starved Chlamydomonas reinhardtii cultures

Prospects for Photobiological Hydrogen as a Renewable Energy

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Comparative analyses of H₂ photoproduction in magnesium‐ and sulfur‐starved Chlamydomonas reinhardtii cultures