Pathways of Apoptosis Regulation in Hepatocytes Induced by First-Line Antitubercular Drugs

Бажанова, Е. Д.; Суханов, Дмитрий Сергеевич; Teplyi, D. L.

doi:10.1007/s10517-015-2828-6

Cited by 4 publications

(5 citation statements)

References 7 publications

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“…Inhibition of P53 and its nuclear translocation by IL-17 impairs apoptosis of the Mtb-infected cells via reduction of caspase-3, Bax, and cytochrome-c release, as well as by increasing Bcl2 [ 170 ]. The ethanolic extract of Pluchea indica and other antitubercular drugs exhibited anti-tuberculosis and anti-inflammatory effects by P53-mediated apoptosis [ 171 , 172 ].…”

Section: Copdmentioning

confidence: 99%

P53 in the impaired lungs

Uddin

Barabutis

2020

DNA Repair

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Our laboratory is focused on investigating the supportive role of P53 towards the maintenance of lung homeostasis. Acute lung injury, acute respiratory distress syndrome, chronic obstructive pulmonary disease, pulmonary fibrosis, bronchial asthma, pulmonary arterial hypertension, pneumonia and tuberculosis are respiratory pathologies, associated with dysfunctions of this endothelium defender (P53). Herein we review the evolving role of P53 towards the aforementioned inflammatory disorders, to potentially reveal new therapeutic possibilities in pulmonary disease.

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Section: Copdmentioning

confidence: 99%

P53 in the impaired lungs

Uddin

Barabutis

2020

DNA Repair

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“…The mechanism of ATD-induced DILI involves both the external apoptosis pathway mediated by the cell surface receptor Fas and the internal apoptotic pathway, which is p53-dependent. Simultaneously, the level of antiapoptotic Bcl-2 protein was reduced 141. Decreased intracellular antioxidases (eg, superoxide dismutase) and elevated ROS generation also play a role 139…”

Section: Hepatocyte Apoptosis and Drug-induced Liver Injury (Dili)mentioning

confidence: 99%

“…140 Combined administration of isoniazid (50 mg/kg subcutaneously), rifampicin (250 mg/kg intragastrically), and pyrazinamide (45 mg/kg intragastrically) for 14 days in rats significantly induced hepatocyte apoptosis. 141 The mechanism of ATD-induced DILI involves both the external apoptosis pathway mediated by the cell surface receptor Fas and the internal apoptotic pathway, which is p53-dependent. Simultaneously, the level of antiapoptotic Bcl-2 protein was reduced.…”

Section: Hepatocyte Apoptosis and Drug-induced Liver Injury (Dili)mentioning

confidence: 99%

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Mechanism of Hepatocyte Apoptosis

et al. 2016

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Hepatocyte apoptosis plays important roles in both the removal of external microorganisms and the occurrence and development of liver diseases. Different conditions, such as virus infection, fatty liver disease, hepatic ischemia reperfusion, and drug-induced liver injury, are accompanied by hepatocyte apoptosis. This review summarizes recent research on the mechanism of hepatocyte apoptosis involving the classical extrinsic and intrinsic apoptotic pathways, endoplasmic reticulum stress, and oxidative stress-induced apoptosis. We emphasized the major causes of apoptosis according to the characteristics of different liver diseases. Several concerns regarding future research and clinical application are also raised.

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“…Expression levels of important functional proteins are typically altered during cell apoptosis ( 33 – 35 ). The results of the present study, using 2D analysis, revealed a significant decrease of 14-3-3ε in the nuclear matrix of apoptotic MG-63 cells.…”

Section: Discussionmentioning

confidence: 99%

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG‑63 cells

et al. 2017

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The 14-3-3 protein family may regulates protein interaction, transportation and cellular localization. The regulatory role of 14-3-3ε is influenced by its altered localization. In the present study, human osteosarcoma MG-63 cells were treated with curcumin to induce apoptosis. Subsequently, the altered expression and localization of 14-3-3ε and its co-localization with other apoptosis-associated proteins during apoptosis was investigated. Analysis of nuclear matrix proteins (NMPs), using two-dimensional gel electrophoresis with matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, revealed that 14-3-3ε existed on the nuclear matrix of MG-63 cells, and its expression was decreased compared with that in control cells following curcumin treatment. In addition, western blot analysis validated that the expression level of 14-3-3ε was downregulated during curcumin-induced apoptosis of MG-63 cells compared with that in control cells. Using immunofluorescence labeling, it was observed that 14-3-3ε was located on the nuclear matrix of MG-63 cells and the distribution of 14-3-3ε on the nuclear matrix was decreased following treatment with curcumin, compared with that in control cells. Double immunofluorescence staining and laser-scanning confocal microscopy demonstrated that 14-3-3ε was co-localized with B-cell lymphoma-2 (Bcl-2), Bcl-2-associated-X protein, p53 and c-FOS transcription factor in MG-63 cells. Furthermore, following treatment with curcumin, these co-localization regions were decreased. The results of the present study revealed that 14-3-3ε is an NMP in MG-63 cells, and its altered expression and co-localization with apoptosis-associated proteins indicated an important function of 14-3-3ε in apoptosis of MG-63 cells. Additional studies are required to investigate the results of the present study.

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Pathways of Apoptosis Regulation in Hepatocytes Induced by First-Line Antitubercular Drugs

Cited by 4 publications

References 7 publications

P53 in the impaired lungs

P53 in the impaired lungs

Mechanism of Hepatocyte Apoptosis

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG‑63 cells

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