Pathways of 2,4,6-Trinitrotoluene Transformation by Aerobic Yeasts

Ziganshin, Ayrat M.; Gerlach, Robin

doi:10.1007/978-3-319-01083-0_14

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…A significant number of land and water territories are contaminated with this explosive and its conversion products as a result of the manufacture, transportation, storage and testing of munitions. These compounds are classified as relatively recalcitrant to biological degradation and their fate is affected by biogeochemical processes (Smets et al 2007 ; Singh et al 2012 ; Ziganshin and Gerlach 2014 ; Chien et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Fe(III) mineral reduction followed by partial dissolution and reactive oxygen species generation during 2,4,6-trinitrotoluene transformation by the aerobic yeast Yarrowia lipolytica

et al. 2015

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Understanding the factors that influence pollutant transformation in the presence of ferric (oxyhydr)oxides is crucial to the efficient application of different remediation strategies. In this study we determined the effect of goethite, hematite, magnetite and ferrihydrite on the transformation of 2,4,6-trinitrotoluene (TNT) by Yarrowia lipolytica AN-L15. The presence of ferric (oxyhydr)oxides led to a small decrease in the rate of TNT removal. In all cases, a significant release of NO2− from TNT and further NO2− oxidation to NO3− was observed. A fraction of the released NO2− was abiotically decomposed to NO and NO2, and then NO was likely oxidized abiotically to NO2 by O2. ESR analysis revealed the generation of superoxide in the culture medium; its further protonation at low pH resulted in the formation of hydroperoxyl radical. Presumably, a fraction of NO released during TNT degradation reacted with superoxide and formed peroxynitrite, which was further rearranged to NO3− at the acidic pH values observed in this study. A transformation and reduction of ferric (oxyhydr)oxides followed by partial dissolution (in the range of 7–86% of the initial Fe(III)) were observed in the presence of cells and TNT. Mössbauer spectroscopy showed some minor changes for goethite, magnetite and ferrihydrite samples during their incubation with Y. lipolytica and TNT. This study shows that i) reactive oxygen and nitrogen species generated during TNT transformation by Y. lipolytica participate in the abiotic conversion of TNT and ii) the presence of iron(III) minerals leads to a minor decrease in TNT transformation.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-014-0094-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Fe(III) mineral reduction followed by partial dissolution and reactive oxygen species generation during 2,4,6-trinitrotoluene transformation by the aerobic yeast Yarrowia lipolytica

et al. 2015

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“…The most commonly used military energetic compounds include the explosive 2,4,6-trinitrotoluene and such propellant ingredients as nitroglycerin and nitrocellulose (cellulose nitrate) (El-Diwani et al 2009;Pichtel 2012;Ziganshin & Gerlach 2014). Nitrocellulose has a special place in the production of various materials for national economic purposes, and this compound belongs to one of the most commonly used substances in military and paint industries.…”

Section: Introductionmentioning

confidence: 99%

Bacterial communities inhabiting toxic industrial wastewater generated during nitrocellulose production

et al. 2016

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Investigating the microbial community structure and composition of toxic industrial wastes contaminated with nitrocellulose and various by-products is crucial for understanding the fate of these pollutants in the environment and for the development and application of effective bioremediation processes. In this study, we investigated the chemical properties and toxic potential of wastewater generated during nitrocellulose production. The analyzed wastewater from settling pond contained nitrocellulose powder particles as well as increased ammonium (570-760 mg/L), sulfate (1625-2045 mg/L) and sulfite (864-1014 mg/L) concentrations. The toxicity test results demonstrated that the wastewater samples present acute toxicity for Paramecium caudatum and Daphnia magna. Furthermore, bacterial community structure in the samples was characterized by pyrosequencing of 16S rRNA genes. Phylogenetic analysis of bacterial sequences indicated that Proteobacteria, Bacteroidetes and Firmicutes were the main phyla in the sample near inlet, whereas various phylotypes of the phyla Proteobacteria, Chlorobi, Bacteroidetes and Gemmatimonadetes dominated in the sample near outlet. Some bacterial members observed in the current work can be considered as agents capable of performing biodegradation of various hazardous contaminants, indicating that the described bacterial communities have a high potential for the development of effective bioremediation strategies.

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“…The microbial degradation of TNT through the formation of TNT-Meisenheimer complexes is a unique path leading to the release of nitrogroups and conditioning the enzymatic cleavage of the aromatic ring, which is followed by its complete degradation ( Ziganshin and Gerlach, 2014 ). Enterobacter cloacae PB2, Pseudomonas fluorescence I-C, and Pseudomonas putida JLR11 are the aerobic bacteria that hydrogenate the TNT aromatic ring and trigger the release of

by the family of old yellow enzymes (OYEs) ( Pak et al, 2000 ; Wittich et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of 2,4,6-Trinitrotoluene Degrading Yeast Yarrowia lipolytica

2017

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2,4,6-trinitrotoluene (TNT) is a common component of many explosives. The overproduction and extensive usage of TNT significantly contaminates the environment. TNT accumulates in soils and aquatic ecosystems and can primarily be destroyed by microorganisms. Current work is devoted to investigation of Yarrowia lipolytica proteins responsible for TNT transformation through the pathway leading to protonated Meisenheimer complexes and nitrite release. Here, we identified a unique set of upregulated membrane and cytosolic proteins of Y. lipolytica, which biosynthesis increased during TNT transformation through TNT-monohydride-Meisenheimer complexes in the first step of TNT degradation, through TNT-dihydride-Meisenheimer complexes in the second step, and the aromatic ring denitration and degradation in the last step. We established that the production of oxidoreductases, namely, NADH flavin oxidoreductases and NAD(P)+-dependent aldehyde dehydrogenases, as well as transferases was enhanced at all stages of the TNT transformation by Y. lipolytica. The up-regulation of several stress response proteins (superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase) was also detected. The involvement of intracellular nitric oxide dioxygenase in NO formation during nitrite oxidation was shown. Our results present at the first time the full proteome analysis of Y. lipolytica yeast, destructor of TNT.

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Pathways of 2,4,6-Trinitrotoluene Transformation by Aerobic Yeasts

Cited by 4 publications

References 33 publications

Fe(III) mineral reduction followed by partial dissolution and reactive oxygen species generation during 2,4,6-trinitrotoluene transformation by the aerobic yeast Yarrowia lipolytica

Fe(III) mineral reduction followed by partial dissolution and reactive oxygen species generation during 2,4,6-trinitrotoluene transformation by the aerobic yeast Yarrowia lipolytica

Bacterial communities inhabiting toxic industrial wastewater generated during nitrocellulose production

Proteomic Analysis of 2,4,6-Trinitrotoluene Degrading Yeast Yarrowia lipolytica

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