Pathways for membrane trafficking during cytokinesis

Strickland, Laila

doi:10.1016/j.tcb.2004.01.006

Cited by 71 publications

(77 citation statements)

References 36 publications

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“…Membrane trafficking is an essential part of cytokinesis for delivering and regulating the addition of new membrane along the ingressing cleavage furrow (Strickland and Burgess, 2004;Albertson et al, 2005). The importance of membrane fusion events during cytokinesis is highlighted by the requirement of soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins (Low et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…The process of cytokinesis is driven by the formation and constriction of an actomyosin II contractile ring and by targeted membrane addition (Glotzer, 2005). Membrane delivery is first required during cleavage furrow ingression to increase the surface area of the two daughter cells and later during the abscission process to seal the two newly formed cells (Strickland and Burgess, 2004;Albertson et al, 2005). Although membrane vesicles delivered into the cleavage furrow were thought to be derived from the secretory pathway (Skop et al, 2001), endocytosis and membrane recycling have also now been shown to be required for cytokinesis (Gerald et al, 2001;Schweitzer and D'SouzaSchorey, 2002;Fielding et al, 2005;Wilson et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Myosin VI Is Required for Targeted Membrane Transport during Cytokinesis

Arden

Puri

et al. 2007

MBoC

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Myosin VI plays important roles in endocytic and exocytic membrane-trafficking pathways in cells. Because recent work has highlighted the importance of targeted membrane transport during cytokinesis, we investigated whether myosin VI plays a role in this process during cell division. In dividing cells, myosin VI undergoes dramatic changes in localization: in prophase, myosin VI is recruited to the spindle poles; and in cytokinesis, myosin VI is targeted to the walls of the ingressing cleavage furrow, with a dramatic concentration in the midbody region. Furthermore, myosin VI is present on vesicles moving into and out of the cytoplasmic bridge connecting the two daughter cells. Inhibition of myosin VI activity by small interfering RNA (siRNA)-mediated knockdown or by overexpression of dominant-negative myosin VI tail leads to a delay in metaphase progression and a defect in cytokinesis. GAIP-interacting protein COOH terminus (GIPC), a myosin VI binding partner, is associated with the function(s) of myosin VI in dividing cells. Loss of GIPC in siRNA knockdown cells results in a more than fourfold increase in the number of multinucleated cells. Our results suggest that myosin VI has novel functions in mitosis and that it plays an essential role in targeted membrane transport during cytokinesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Myosin VI Is Required for Targeted Membrane Transport during Cytokinesis

Arden

Puri

et al. 2007

MBoC

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“…This process requires new membrane insertion at the division site in animal, fungal, plant, and perhaps bacterial cells [Hales et al, 1999;Strickland and Burgess, 2004;Oliferenko et al, 2009]. In budding yeast, immediately after the launch of a new cell cycle in late G1, the growth machinery including the polarity regulators such as Cdc42, the actin cytoskeleton, and the secretory pathways are all directed toward the bud cortex to drive bud emergence and bud enlargement while the mother cell stays constant in size [Bi and Park, 2012].…”

Section: Targeted Membrane Deposition Septum Formation and Cell Sepmentioning

confidence: 99%

Mechanisms of cytokinesis in budding yeast

Wloka

2012

Cytoskeleton

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Cytokinesis is essential for cell proliferation in all domains of life. Because the core components and mechanisms of cytokinesis are conserved from fungi to humans, the budding yeast Saccharomyces cerevisiae has served as an attractive model for studying this fundamental process. Cytokinesis in budding yeast is driven by two interdependent cellular events: actomyosin ring (AMR) constriction and the formation of a chitinous cell wall structure called the primary septum (PS), the functional equivalent of extracellular matrix remodeling during animal cytokinesis. AMR constriction is thought to drive efficient plasma membrane ingression as well as to guide PS formation, whereas PS formation is thought to stabilize the AMR during its constriction. Following the completion of the PS formation, two secondary septa (SS), consisting of glucans and mannoproteins, are synthesized at both sides of the PS. Degradation of the PS and a part of the SS by a chitinase and glucanases then enables cell separation. In this review, we discuss the mechanics of cytokinesis in budding yeast, highlighting its common and unique features as well as the emerging questions. © 2012 Wiley Periodicals, Inc.

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“…Septins are required for cytokinesis as well as for exocytosis in neuron (Beites et al, 1999;Field and Kellogg, 1999). Several proteins needed for internalization and endocytosis also are required for cytokinesis and/or cellularization in different species, such as syntaxins (t-SNARE), dynamin, clathrin, the clathrin adaptor AP-2, and some Rab proteins, Rab3 in sea urchin, and Rab11 and the Rab11 effector Nuf in Drosophila (O'Halloran, 2000;Finger and White, 2002;Strickland and Burgess, 2004). Furthermore, it seems that membrane fusion events are possibly linked with actin remodeling (Burgess et al, 1997;Niswonger and O'Halloran, 1997, Emoto andUmeda, 2000).…”

Section: Introductionmentioning

confidence: 99%

Clues to CD2-associated Protein Involvement in Cytokinesis

Monzo

Gauthier

Keslair

et al. 2005

MBoC

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Cytokinesis requires membrane trafficking coupled to actin remodeling and involves a number of trafficking molecules. CD2-associated protein (CD2AP) has been implicated in dynamic actin remodeling and membrane trafficking that occurs during endocytosis leading to the degradative pathway. In this study, we present several arguments for its implication in cytokinesis. First, endogenous CD2AP was found concentrated in the narrow region of the midzone microtubules during anaphase and in the midbody during late telophase. Moreover, we found that CD2AP is a membrane-and not a microtubule-associated protein. Second, the overexpression of the first two Src homology 3 domains of CD2AP, which are responsible for this localization, led to a significant increase in the rate of cell multinucleation. Third, the CD2AP small interfering RNA interfered with the cell separation, indicating that CD2AP is required for HeLa cells cytokinesis. Fourth, using the yeast two-hybrid system, we found that CD2AP interacted with anillin, a specific cleavage furrow component, and the two proteins colocalized at the midbody. Both CD2AP and anillin were found phosphorylated early in mitosis and also CD2AP phosphorylation was coupled to its delocalization from membrane to cytosol. All these observations led us to propose CD2AP as a new player in cytokinesis. INTRODUCTIONCytokinesis is a fundamental cellular process that leads to the separation of daughter cells after mitosis. This mechanism involves microtubules and actin remodeling that are, in part, controlled by phosphorylation, degradation, and membrane fusion events (Robinson and Spudich, 2000;Straight and Field, 2000;Glotzer, 2001). During this process, dividing cells form a plasma membrane invagination or cleavage furrow, which is created by an actomyosin-based contractile ring that assembles under the plasma membrane. At a late stage of cytokinesis a narrow cytoplasmic bridge, defined as the midbody, connects the two daughter cells. This bridge is ultimately resolved and the two cells separate, thus completing cytokinesis.Furrow ingression is accompanied by fusion of membrane vesicles with the ingressing plasma membrane (O'Halloran, 2000;Finger and White, 2002). A number of membrane trafficking proteins were found at the division site. Septins are required for cytokinesis as well as for exocytosis in neuron (Beites et al., 1999;Field and Kellogg, 1999). Several proteins needed for internalization and endocytosis also are required for cytokinesis and/or cellularization in different species, such as syntaxins (t-SNARE), dynamin, clathrin, the clathrin adaptor AP-2, and some Rab proteins, Rab3 in sea urchin, and Rab11 and the Rab11 effector Nuf in Drosophila (O'Halloran, 2000;Finger and White, 2002;Strickland and Burgess, 2004). Furthermore, it seems that membrane fusion events are possibly linked with actin remodeling (Burgess et al., 1997;Niswonger and O'Halloran, 1997, Emoto andUmeda, 2000). Indeed, in cellularizing Drosophila embryo mutants for syntaxin 1, a protein that is essential fo...

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Pathways for membrane trafficking during cytokinesis

Cited by 71 publications

References 36 publications

Myosin VI Is Required for Targeted Membrane Transport during Cytokinesis

Myosin VI Is Required for Targeted Membrane Transport during Cytokinesis

Mechanisms of cytokinesis in budding yeast

Clues to CD2-associated Protein Involvement in Cytokinesis

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