Pathways for eosinophil lipid body induction: differing signal transduction in cells from normal and hypereosinophilic subjects

Bozza, Patrı́cia T.; Yu, Wengui; Cassara, Jessica; Weller, Peter F.

doi:10.1002/jlb.64.4.563

Cited by 64 publications

(69 citation statements)

References 25 publications

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“…25) For instance, lipid body numbers are increased in eosinophils from patients with hypereosinophilic syndrome, 26) in leukocytes from inflammatory arthritis 25) and sepsis, 8) and from bronchoalveolar lavage of patients with acute respiratory distress syndrome, 27,28) Lipid bodies are candidates to play a major role in the formation of eicosanoid mediators during inflammation as they are sites of localization of several enzymes related to eicosanoid metabolism including COX-2, 5-lipoxygenase, phospholipase A2, mitogen-activated protein kinase and phosphatidylinositide 3-kinase. 25,[29][30][31] In addition, agents that inhibit lipid body formation in vitro inhibited the priming response for enhanced eicosanoid release.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity in the Aqueous Crude Extract of the Leaves of Nidularium procerum: A Bromeliaceae from the Brazilian Coastal Rain Forest

Amendoeira¹,

Frutuoso²,

Zanon³

et al. 2005

Biological & Pharmaceutical Bulletin

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Brazilian flora, especially in the Brazilian coastal rain forest, remains poorly studied regarding its chemical and pharmacological potential. A recent study published by our group indicates that Nidularium procerum, the most common representative of the bromeliaceae family, has potent analgesic and anti-inflammatory activities.1) Bromeliaceae, one of the largest botanical families of the New World, is extensively distributed in tropical America.2) This family comprises 46 genera encompassing about 2500 species from which 20 genera and 245 species are found in the State of Rio de Janeiro. The Brazilian coastal rain forest is the preferred habitat of Bromeliaceae (81.8%) where the greatest genetic diversity and the highest degree of endemism is found.3) Species of Nidularium are well spread in the coastal rain forest from Bahia State, in the northeast region of Brazil, to Rio Grande do Sul State in the south. 4)Our previous results indicate that N. procerum extracts have strong analgesic activity, which is not associated with the activation of opioid receptors, but is mechanistically due to a selective inhibition of PGE 2 production.1) In addition, we showed that the aqueous extract of N. procerum leaves (LAE) inhibited LPS-induced neutrophil accumulation in the mouse pleural cavity, but the molecular mechanism for this anti-inflammatory effect was not investigated.1) Here, we investigated the mechanisms involved in the anti-inflammatory effects of N. procerum aqueous extract on the LPS-induced pleurisy model. We showed that in addition to LPS-induced neutrophil influx, N. procerum also inhibited LPS-driven in vivo neutrophil activation (as attested by inhibition of lipid body formation) and PAF-and fMLP-induced pleural neutrophilia. The mechanisms involved in N. procerum-mediated inhibition of neutrophil recruitment appear to involve decreased production of key inflammatory mediators of LPSdriven response, such as PGE 2 and inflammatory cytokines (IL-1 and IL-6), and an impaired locomotory activity of migrating neutrophils. MATERIALS AND METHODS Plant MaterialNidularium procerum LINDMAN was collected at the National Biological Reserve of Poço das Antas, Silva Jardim, Rio de Janeiro State, Brazil. Identification of the botanical material was made by Dr. Tânia Wendt and Dr. Elton Leme and a voucher has been deposited at the Herbarium of the Rio de Janeiro Botanical Garden, under the number 304732.Preparation of Plant Extracts The aqueous extract of leaves (LAE) (22 g) was obtained by infusing dried leaves (500 g) into 2.5 l of boiling distilled water until the water cooled down to room temperature, followed by filtration and lyophilization as previously described. 1)Animals Male Swiss mice (obtained from the Oswaldo Cruz Foundation breeding unit) weighing 20-30 g, were used. The animals were maintained with free access to food and water and kept at 25-28°C with controlled 12 h light/dark cycle at the Department of Physiology and Pharmacodynamics. The experiments in this study received prior approval from the Oswal...

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity in the Aqueous Crude Extract of the Leaves of Nidularium procerum: A Bromeliaceae from the Brazilian Coastal Rain Forest

Amendoeira¹,

Frutuoso²,

Zanon³

et al. 2005

Biological & Pharmaceutical Bulletin

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“…PAF-like lipids are potent proinflammatory mediators and seem to be involved on earliest events of atherosclerosis in mice because the PAF receptor antagonist, WEB 2086, decreased monocyte-endothelial interactions and fatty streak formation in LDL receptor-deficient animals fed a lipid-rich diet (19). It is important to note that authentic PAF induces lipid body formation in leukocytes such as neutrophils (20) and eosinophils (21,22). Those lipid bodies are membraneless lipid-rich cytoplasmic inclusions localized in leukocytes and in other inflammation-associated cell types.…”

mentioning

confidence: 99%

Synergism Between Platelet-Activating Factor-Like Phospholipids and Peroxisome Proliferator-Activated Receptor γ Agonists Generated During Low Density Lipoprotein Oxidation That Induces Lipid Body Formation in Leukocytes

Assis

Silva

Caiado

et al. 2003

The Journal of Immunology

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Oxidized low density lipoprotein (LDL) has an important proinflammatory role in atherogenesis. In this study, we investigated the ability of oxidized LDL (oxLDL) and its phospholipid components to induce lipid body formation in leukocytes. Incubation of mouse peritoneal macrophages with oxidized, but not with native LDL led to lipid body formation within 1 h. This was blocked by platelet-activating factor (PAF) receptor antagonists or by preincubation of oxLDL with rPAF acetylhydrolase. HPLC fractions of phospholipids purified from oxLDL induced calcium flux in neutrophils as well as lipid body formation in macrophages. Injection of the bioactive phospholipid fractions or butanoyl and butenoyl PAF, a phospholipid previously shown to be present in oxLDL, into the pleural cavity of mice induced lipid body formation in leukocytes recovered after 3 h. The 5-lipoxygenase and cyclooxygenase-2 colocalized within lipid bodies formed after stimulation with oxLDL, bioactive phospholipid fractions, or butanoyl and butenoyl PAF. Lipid body formation was inhibited by 5-lipoxygenase antagonists, but not by cyclooxygenase-2 inhibitors. Azelaoyl-phosphatidylcholine, a peroxisome proliferator-activated receptor-γ agonist in oxLDL phospholipid fractions, induced formation of lipid bodies at late time points (6 h) and synergized with suboptimal concentrations of oxLDL. We conclude that lipid body formation is an important proinflammatory effect of oxLDL and that PAF-like phospholipids and peroxisome proliferator-activated receptor-γ agonists generated during LDL oxidation are important mediators in this phenomenon.

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“…Diferente dos adipócitos, nos leucócitos a formação de CLs é específica e dependente do estímulo [76][77][78] , do tipo celular [79] e de receptores [77,[80][81][82] . Os CLs são tipicamente escassos em leucócitos não ativados, mas caracteristicamente aumentados em número e tamanho in vitro e in vivo em células inflamatórias [76,[82][83][84][85][86] .…”

Section: Corpúsculos Lipídicos E Sua Inibição Farmacológicaunclassified

“…Trabalhos da literatura já demonstraram uma relação entre a produção inicial de eicosanóides derivados da 5-LO, COX-1 e 2 e a formação CLs in vitro [80,[135][136][137][138][139][140] . Estas são estruturas intracelulares ricas em lipídios não associadas à membrana, cuja indução é específica, dependente do estímulo, [77,80] do tipo celular [79] e do receptor [77,80,94] .…”

Section: Infecção Com As Cepas Sv009 E Sv068 Induz Produção In Vivo Deunclassified

“…Estas são estruturas intracelulares ricas em lipídios não associadas à membrana, cuja indução é específica, dependente do estímulo, [77,80] do tipo celular [79] e do receptor [77,80,94] . [76,77,94] . Africa-2 e Somalia-2, estão relacionadas com maior ou menor carga bacilar nos pulmões [141] .…”

Section: Infecção Com As Cepas Sv009 E Sv068 Induz Produção In Vivo Deunclassified

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Avaliação da participação de mediadores lipídicos nas infecções experimentais induzidas por diferentes isolados de Mycobacterium tuberculosis de humanos

Soares¹

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Jacob Soares, responsáveis pelo que me tornei; aos meus irmãos Junior e Eduardo, pelo amor e companheirismo que nos une. Em especial ao Eduardo que lá no 'fundo' sempre teve interesse na ciência e vinha sempre com alguma pergunta do tipo: "Tata, o que é que você estuda?"; "Que experimento você faz?" e das perguntas sempre vinham as suas exclamações imediatas encantado com minhas respostas: "Nossa Tata, que tudo!!!""O primeiro passo para conseguir algo é desejá-lo". Eleuza, Bete, Daniela Isabel, Walter Turato (Tatu), Daniela Carlos, Brunão, Cláudia (Calúdia), Daiane, Beto, Romulo, Fran, Laís, Fabiana (FACs) Madre Teresa de Calcutá" Figura 5. Tratamento com MK886 não altera a produção de nitrito e de TNF- por MAs infectados com as cepas SV009 ou SV068Pág. 66 Figura 6. Efeito do tratamento com ácido caféico na produção de nitrito e TNF- por MAs infectados com as cepas SV009 ou SV068 Pág. 68 Figura 7. Atividades fagocítica e microbicida de MAs são diferentes após infecção com as cepas SV068 e SV009 Pág. 70 Figura 8. Unidades formadoras de colônias recuperadas de MAs infectados após tratamento com MK886 ou ácido caféico Pág. 72 Figura 9. Tratamento com MK886 diminui parcialmente a formação de CLs em macrófagos infectados com a cepa SV068 Pág. 74 Figura 10. Efeito do tratamento com ácido caféico na formação de CLs por MAs infectados com as cepas SV009 e SV068 Pág. 76 Figura 11. Curvas de sobrevivência de camundongos Balb/c infectados com as cepas SV009 ou SV068 e tratados ou não com MK886 Pág. 80 Figura 12. Carga bacilar nos pulmões e baço de camundongos balb/c infectados com as cepas SV009 ou SV068 de Mtb Pág. 83 Figura 13. Fotomicrografias representativas do tecido pulmonar de camundongos Balb/c corados com Hematoxilina&Eosina, Tricrômio de Gomori e Ziehl Neelsen 30 e 60 dias após a infecção com as cepas de Mtb Pág. 86 Figura 14. Cinética do recrutamento de células inflamatórias para o espaço broncoalveolar de camundongos balb/c infectados com bacilos das cepas SV009 e SV068 de Mtb, e tratados ou não com MK886 Pág. 91 Figura 15. Óxido nítrico produzido nos pulmões de camundongos balb/c infectados com as cepas de Mtb Pág. 94 Figura 16. Citocinas produzidas pelas células do parênquima pulmonar de camundongos balb/c infectados ou não com as duas cepas de Mtb

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Pathways for eosinophil lipid body induction: differing signal transduction in cells from normal and hypereosinophilic subjects

Cited by 64 publications

References 25 publications

Anti-inflammatory Activity in the Aqueous Crude Extract of the Leaves of Nidularium procerum: A Bromeliaceae from the Brazilian Coastal Rain Forest

Anti-inflammatory Activity in the Aqueous Crude Extract of the Leaves of Nidularium procerum: A Bromeliaceae from the Brazilian Coastal Rain Forest

Synergism Between Platelet-Activating Factor-Like Phospholipids and Peroxisome Proliferator-Activated Receptor γ Agonists Generated During Low Density Lipoprotein Oxidation That Induces Lipid Body Formation in Leukocytes

Avaliação da participação de mediadores lipídicos nas infecções experimentais induzidas por diferentes isolados de Mycobacterium tuberculosis de humanos

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