Pathways for arsenic from sediments to groundwater to streams: Biogeochemical processes in the Inner Coastal Plain, New Jersey, USA

Barringer, Julia L.; Mumford, Adam C.; Young, L. Y.; Reilly, Pamela A.; Bonin, Jennifer L.; Rosman, Robert

doi:10.1016/j.watres.2010.05.047

Cited by 48 publications

(37 citation statements)

References 41 publications

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“…2A and B), supporting the model of coupled reductive dissolution of iron minerals and As release to solution (10,27,35,(60)(61)(62)(63)(64)(65). A strong correlation between the increases in the concentrations of Fe II and As III for the NP3 sediment amended with glucose suggests that the reduction of As V was likely due to the direct activity of DARB on both adsorbed arsenic and that released into solution by the reduction of Fe III .…”

Section: Figsupporting

confidence: 63%

“…For ease of presentation, we have reported only the results of the DNA sequences at the 90% similarity level (Fig. 5), even though cutoff levels as low as 85% similarity have been used for the clustering of this gene (27). Overall, the 288 arrA gene sequences from this study could be grouped into 14 distinctive phylotypes (clusters 1 to 14) and four singletons (Fig.…”

Section: Resultsmentioning

confidence: 92%

“…However, to obtain information about the potential As reduction function associated with these microorganisms requires the sequencing of the functional gene arrA. Previously, the arrA gene has been used to assess the diversity of DARB in a range of ecosystems, including water and sediments from Mono Lake and Searles Lake, CA (20)(21)(22), arsenic-contaminated aquifers in Cambodia (23,24) and West Bengal (25), estuarine sediments from Chesapeake Bay (26), and streambed sediments from the Inner Coastal Plain, NJ (27,28). However, information on the reduction of As V in relation to Fe reduction and the microbial community (both structural and functional) responsible for these reactions within the basin-fill environment of the Southwestern United States has not been reported.…”

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confidence: 99%

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Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah

Mirza

Muruganandam

Meng

et al. 2014

Appl Environ Microbiol

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Basin-fill aquifers of the Southwestern United States are associated with elevated concentrations of arsenic (As) in groundwater. Many private domestic wells in the Cache Valley Basin, UT, have As concentrations in excess of the U.S. EPA drinking water limit. Thirteen sediment cores were collected from the center of the valley at the depth of the shallow groundwater and were sectioned into layers based on redoxmorphic features. Three of the layers, two from redox transition zones and one from a depletion zone, were used to establish microcosms. Microcosms were treated with groundwater (GW) or groundwater plus glucose (GW؉G) to investigate the extent of As reduction in relation to iron (Fe) transformation and characterize the microbial community structure and function by sequencing 16S rRNA and arsenate dissimilatory reductase (arrA) genes. Under the carbon-limited conditions of the GW treatment, As reduction was independent of Fe reduction, despite the abundance of sequences related to Geobacter and Shewanella, genera that include a variety of dissimilatory iron-reducing bacteria. The addition of glucose, an electron donor and carbon source, caused substantial shifts toward domination of the bacterial community by Clostridium-related organisms, and As reduction was correlated with Fe reduction for the sediments from the redox transition zone. The arrA gene sequencing from microcosms at day 54 of incubation showed the presence of 14 unique phylotypes, none of which were related to any previously described arrA gene sequence, suggesting a unique community of dissimilatory arsenate-respiring bacteria in the Cache Valley Basin.A rsenic (As) is one of the most frequently detected contaminants in private domestic wells used for household drinking water (1) and public water supplies (2). In the United States, As concentrations in excess of the drinking water maximum contaminant level (MCL; 10 g/liter) in public supply wells are distributed across the country, but 3/4 of these wells are in the western United States (2). In basin-fill aquifers in California, Nevada, New Mexico, Arizona (1), and Utah (3), 10% of the domestic wells tested had As in excess of the MCL. Modeling efforts by the USGS predicted that 43% of the area in the basin-fill aquifers of the Southwest might have groundwater that equals or exceeds the MCL for As (4). While public water suppliers are required to treat water to meet drinking water standards, there are no such requirements for private wells.Common aspects of the basin-fill aquifers in the western United States are geothermal activity and volcanic rock. The source of As in the Cache Valley Basin, UT, is presumed to be the hydrothermal sulfide and arsenide deposits in the surrounding mountains. Due to low rainfall and high evapotranspiration, groundwater recharge does not result from precipitation in the basin but from precipitation in the surrounding mountains. The Cache Valley Basin is located 128 km northeast of Salt Lake City and is on the eastern edge of the Basin and Range Province. A sur...

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Section: Figsupporting

confidence: 63%

Section: Resultsmentioning

confidence: 92%

mentioning

confidence: 99%

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Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah

Mirza

Muruganandam

Meng

et al. 2014

Appl Environ Microbiol

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“…Most of the previous studies that focused on the assessment of arrA gene diversity across different environments, including arseniccontaminated Cambodian sediments (32), aquifer sediments from West Bengal, India (33), estuarine sediments from the Chesapeake Bay (20), streambed sediments from the Inner Coastal Plain in New Jersey (21,34), water and sediments from Mono Lake and Searles Lake in California (35,36), sediments from Rifle, Colorado (37), and sediments from the CVB (17), used a preenrichment step before the evaluation of arrA gene diversity. Some studies (12,25,38) were able to amplify arrA gene sequences directly from environmental samples using existing primers, without preenrichment, as recently highlighted by Ying et al (25).…”

Section: Resultsmentioning

confidence: 99%

New Arsenate Reductase Gene ( arrA ) PCR Primers for Diversity Assessment and Quantification in Environmental Samples

Mirza

Sorensen

Dupont

et al. 2017

Appl Environ Microbiol

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The extent of arsenic contamination in drinking water and its potential threat to human health have resulted in considerable research interest in the microbial species responsible for arsenic reduction. The arsenate reductase gene (arrA), an important component of the microbial arsenate reduction system, has been widely used as a biomarker to study arsenate-reducing microorganisms. A new primer pair was designed and evaluated for quantitative PCR (qPCR) and high-throughput sequencing of the arrA gene, because currently available PCR primers are not suitable for these applications. The primers were evaluated in silico and empirically tested for amplification of arrA genes in clones and for amplification and high-throughput sequencing of arrA genes from soil and groundwater samples. In silico, this primer pair matched (Ն90% DNA identity) 86% of arrA gene sequences from GenBank. Empirical evaluation showed successful amplification of arrA gene clones of diverse phylogenetic groups, as well as amplification and high-throughput sequencing of independent soil and groundwater samples without preenrichment, suggesting that these primers are highly specific and can amplify a broad diversity of arrA genes. The arrA gene diversity from soil and groundwater samples from the Cache Valley Basin (CVB) in Utah was greater than anticipated. We observed a significant correlation between arrA gene abundance, quantified through qPCR, and reduced arsenic (As III ) concentrations in the groundwater samples. Furthermore, we demonstrated that these primers can be useful for studying the diversity of arsenate-reducing microbial communities and the ways in which their relative abundance in groundwater may be associated with different groundwater quality parameters. IMPORTANCE Arsenic is a major drinking water contaminant that threatens the health of millions of people worldwide. The extent of arsenic contamination and its potential threat to human health have resulted in considerable interest in the study of microbial species responsible for the reduction of arsenic, i.e., the conversion of As V to As III . In this study, we developed a new primer pair to evaluate the diversity and abundance of arsenate-reducing microorganisms in soil and groundwater samples from the CVB in Utah. We observed significant arrA gene diversity in the CVB soil and groundwater samples, and arrA gene abundance was significantly correlated with the reduced arsenic (As III ) concentrations in the groundwater samples. We think that these primers are useful for studying the ecology of arsenate-reducing microorganisms in different environments.KEYWORDS arsenate reductase gene (arrA), high-throughput sequencing, qPCR of arrA gene, arsenic reduction

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“…2, sample IMCUGSH15 which had the highest As concentration also showed with the highest TOC content. This might imply that sediments with high contents of TOC might be in favor of microbial mediated As enrichment (Barringer et al 2010). DGGE analysis DGGE patterns indicated that bacterial community in high As sediments was dynamically diversified with increasing depth, which could be due to the different distribution of geochemical parameters with depth.…”

Section: Sediment Geochemistrymentioning

confidence: 99%

Vertical distribution of bacterial communities in high arsenic sediments of Hetao Plain, Inner Mongolia

Wang

Jiang

et al. 2014

Ecotoxicology

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Vertical distribution of bacterial communities was detected in high arsenic (As) sediments in a representative high As area in Inner Mongolia. Nineteen sediment samples were collected from a 30 m borehole and detected by geochemistry and molecular ecological approaches including polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), 16S rRNA gene clone library and 454 pyrosequencing. As contents ranged from 42.1 to 111.3 mg kg(-1) which fluctuated with different depth and significantly high in clay and mild clay sediment samples at depth of 8, 20, 25 and 28 m respectively. The ratios of As(III) to total As generally increased with depth but As(V) dominated in all sediment samples. High concentrations of total As, sulfur, iron and total organic carbon were generally found in clay and low in sand samples. Both DGGE patterns and 454 pyrosequencing results indicated that bacterial communities dynamically diversified with increasing depth and were dominated by Firmicutes, Bacteroidetes, Proteobacteria and Chloroflexi. Most of the sediment samples were dominated by populations including Sporosarcina, Acinetobacter, Pseudomonas, Halomonas, Polaromonas, Paenibacillus and Flavobacterium. These populations were found with high similarities with those microbes capable of denitrification, sulfur oxidation, organic matter degradation and As resistance and reduction. These results implied that microbes might play an important role in As mobilization in the shallow aquifers of Hetao Plain, Inner Mongolia.

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Pathways for arsenic from sediments to groundwater to streams: Biogeochemical processes in the Inner Coastal Plain, New Jersey, USA

Cited by 48 publications

References 41 publications

Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah

Arsenic(V) Reduction in Relation to Iron(III) Transformation and Molecular Characterization of the Structural and Functional Microbial Community in Sediments of a Basin-Fill Aquifer in Northern Utah

New Arsenate Reductase Gene ( arrA ) PCR Primers for Diversity Assessment and Quantification in Environmental Samples

Vertical distribution of bacterial communities in high arsenic sediments of Hetao Plain, Inner Mongolia

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