Pathways and genes involved in steroid hormone metabolism in male pigs: A review and update

Robic, Annie; Faraut, Thomas; Prunier, Armelle

doi:10.1016/j.jsbmb.2013.11.001

Cited by 42 publications

(47 citation statements)

References 97 publications

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“…The present data enrich the existing knowledge on steroidogenesis pathways occurring in pigs that we have recently described (Robic et al ). This regards especially epiADT and 19‐norTesto synthesis.…”

Section: Discussionsupporting

confidence: 83%

“…The absence of CYP21 in the testis demonstrates the lack of conversion of progesterone into androstadienone via 11‐deoxycortisone, as proposed by Brooks & Pearson (). Two other pathways leading to androstadienone (precursor of androstenone) were already demonstrated in pig testes (Robic et al ). They do not involve CYP21 in the synthesis of androstenone.…”

Section: Discussionmentioning

confidence: 89%

“…As in other mammals, the production of sex steroids in male pigs varies greatly with age and shows two peaks of activity, during the first month of life and around puberty (Schwarzenberger et al ). Nevertheless, this steroid production shows several specificities (Schwarzenberger et al ; Raeside et al ; Robic et al ). Endocrine organs of boars are able to produce four compounds classified as androgens (testosterone, epiandrosterone (epiADT), androstanediol and 19‐nortestosterone (19‐norTesto)), two precursors of androgens or estrogens (DHEA (dehydroepiandrosterone), and androstenedione (Δ4‐AD)), two estrogenic compounds (estrone and estradiol), and androstenone (Schwarzenberger et al ; Raeside et al ; Robic et al ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exploration of steroidogenesis‐related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs

Robic

Fève

Louveau

et al. 2015

Animal Science Journal

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To explore the metabolism of steroids in the pig species, a qualitative PCR analysis was performed for the main transcript of 27 genes involved in steroid metabolism. We compared samples of testes, adipose tissue and liver from immature and peripubertal males, adrenal cortex from peripubertal males, ovaries from cyclic females and adipose tissue from peripubertal females. Some genes were shown to have a tissue-specific expression. Two of them were expressed only in testes, ovaries and adrenals: CYP11A1 and CYP11B. The CYP21 and HSD17B3 genes, were expressed respectively only in adrenals and only in testes. Very few differences were observed between transcriptional patterns of peripubertal testes and adrenal glands as well as between male and female fat tissues. However, the expression of genes involved in the sulfonation of steroids was higher in testes than in adrenals from males. Main differences between ovaries and testes were observed for HSD17B1/2/3, AKR1C-pig6 and sulfotransferase genes (SULT2A1/SULT2B1). The present study shows that the SRD5A2 and CYP21 genes were not involved in the testicular biosynthesis of androstenone. It also shows that porcine adrenal glands produce essentially corticosteroids and that fat tissue is unable to produce de novo steroids.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploration of steroidogenesis‐related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs

Robic

Fève

Louveau

et al. 2015

Animal Science Journal

Self Cite

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show abstract

“…Agranular endoplasmic reticulum, mitochondria and well developed Golgi complex were the most prominent organelles in the cytoplasm of interstitial cells of the contralateral scrotal testes and the testes of the normal bucks. Abundance of these organelles has been demonstrated as a characteristic feature of steroid synthesizing cells (Shima et al, 2013a,b;Robic et al, 2014). It seems logical that the elaborate nature of these organelles in the contralateral scrotal testes, and the observed hyperplasia of interstitial cells are indications of increased steroid secretory and metabolic activities of the interstitial cells.…”

Section: Discussionmentioning

confidence: 94%

Quantitative and cytological studies of interstitial (Leydig) cells in the scrotal and retained testes of unilateral cryptorchid West African Dwarf goats

Okpe

Ezeasor

2016

Small Ruminant Research

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“…StAR, cyp11A1, 3β‐HSD, and HSD17Β4 are key enzymes in steroid hormone biosynthesis (Fukami, Homma, Hasegawa, & Ogata, ; Robic, Faraut, & Prunier, ). The present study evaluated the expression of these enzymes after NRDR was inhibited and found that only HSD17Β4 expression was downregulated.…”

Section: Discussionmentioning

confidence: 99%

NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

Liu

Yang

et al. 2018

Molecular Reproduction Devel

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Cumulus cells secreting steroid hormones have important functions in oocyte development. Several members of the short‐chain dehydrogenase/reductase (SDR) family are critical to the biosynthesis of steroid hormones. NADPH‐dependent retinol dehydrogenase/reductase ( NRDR ), a member of the SDR superfamily, is overexpressed in pig breeds that also show high levels of androstenone. However, the potential functions and regulatory mechanisms of NRDR in pig ovaries have not been reported to date. The present study demonstrated that NRDR is highly expressed in pig ovaries and is specifically located in cumulus granulosa cells. Functional studies showed that NRDR inhibition increased estradiol synthesis. Both pregnant mare serum gonadotropin and human chorionic gonadotropin downregulated the expression of NRDR in pig cumulus granulosa cells. When the relationship between reproductive traits and single‐nucleotide polymorphisms (SNPs) of the NRDR gene was examined, we found that two SNPs affected reproductive traits. SNP rs701332503 was significantly associated with a decrease in the total number of piglets born during multiparity, and rs326982309 was significantly associated with an increase in the average birth weight during primiparity. Thus, NRDR has an important role in steroid hormone biosynthesis in cumulus granulosa cells, and NRDR SNPs are associated with changes in porcine reproduction traits.

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Pathways and genes involved in steroid hormone metabolism in male pigs: A review and update

Cited by 42 publications

References 97 publications

Exploration of steroidogenesis‐related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs

Exploration of steroidogenesis‐related genes in testes, ovaries, adrenals, liver and adipose tissue in pigs

Quantitative and cytological studies of interstitial (Leydig) cells in the scrotal and retained testes of unilateral cryptorchid West African Dwarf goats

NRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs

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