Pathway of propionate formation from ethanol in Pelobacter propionicus

Schink, Bernhard; Kremer, D.R.; Hansen, Theo A.

doi:10.1007/bf00406127

Cited by 65 publications

(37 citation statements)

References 40 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…vG o0 ¼ 3124 kJ per 3 mol ethanol and uses the methylmalonyl-CoA pathway [26]. P. propionicus exhibited di¡erent growth rates and growth yields when growing either with lactate or ethanol.…”

Section: Discussionmentioning

confidence: 99%

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Seeliger

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

Fermentation balances and growth yields were determined with various bacteria fermenting lactate to acetate plus propionate either via methylmalonyl-CoA or via acrylyl-CoA. All strains fermented lactate to acetate plus propionate at approximately a 1:2 ratio. Growth yields of Propionibacterium freudenreichii were more than twice as high as those of Clostridium homopropionicum or Veillonella parvula. Hydrogen was formed as a side product to a significant extent only by V. parvula and Pelobacter propionicus; the latter formed hydrogen preferentially when using ethanol as substrate. Acrylyl-CoA reductase of C. homopropionicum and Clostridium neopropionicum was found nearly exclusively in the cytoplasm thus confirming that this reduction step is unlikely to be involved in energy conservation. C. homopropionicum exhibited higher K S and higher W max values, as well as higher specific substrate turnover rates than P. freudenreichii. The results allow us to conclude that C. homopropionicum using the acrylyl-CoA pathway with low growth yield obtains its specific competitive advantage compared to P. freudenreichii not through higher substrate affinity or metabolic shift toward enhanced acetate-plus-hydrogen formation but through faster specific substrate turnover. ß

show abstract

Section: Discussionmentioning

confidence: 99%

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Seeliger

2002

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…OTU35a was related to Pelobacter propionicus ( Figure 5), which is known to convert ethanol to propionate (3 ethanol+2 bicarbonate → 2 propionate+1 acetate+1 proton+3 water; Schink et al, 1987) but not syntrophically to acetate and H 2 (reaction 11 in Supplementary Table S1) as do Pelobacter acetylenicus and Pelobacter carbinolicus (Schink, 1984;Seitz et al, 1990). However, only small concentrations of propionate were detected in ethanol-supplemented microcosms, whereas transient acetate accumulations were repeatedly observed at 15 and 5°C after the addition of ethanol (Supplementary Figures S4b and S8b).…”

Section: Syntrophic Methanogenesis In Peat O Schmidt Et Almentioning

confidence: 99%

Peat: home to novel syntrophic species that feed acetate- and hydrogen-scavenging methanogens

et al. 2016

View full text Add to dashboard Cite

Syntrophic bacteria drive the anaerobic degradation of certain fermentation products (e.g., butyrate, ethanol, propionate) to intermediary substrates (e.g., H 2 , formate, acetate) that yield methane at the ecosystem level. However, little is known about the in situ activities and identities of these syntrophs in peatlands, ecosystems that produce significant quantities of methane. The consumption of butyrate, ethanol or propionate by anoxic peat slurries at 5 and 15°C yielded methane and CO 2 as the sole accumulating products, indicating that the intermediates H 2 , formate and acetate were scavenged effectively by syntrophic methanogenic consortia. 16S rRNA stable isotope probing identified novel species/strains of Pelobacter and Syntrophomonas that syntrophically oxidized ethanol and butyrate, respectively. Propionate was syntrophically oxidized by novel species of Syntrophobacter and Smithella, genera that use different propionate-oxidizing pathways. Taxa not known for a syntrophic metabolism may have been involved in the oxidation of butyrate (Telmatospirillum-related) and propionate (unclassified Bacteroidetes and unclassified Fibrobacteres). Gibbs free energies (ΔGs) for syntrophic oxidations of ethanol and butyrate were more favorable than ΔGs for syntrophic oxidation of propionate. As a result of the thermodynamic constraints, acetate transiently accumulated in ethanol and butyrate treatments but not in propionate treatments. Aceticlastic methanogens (Methanosarcina, Methanosaeta) appeared to outnumber hydrogenotrophic methanogens (Methanocella, Methanoregula), reinforcing the likely importance of aceticlastic methanogenesis to the overall production of methane. ΔGs for acetogenesis from H 2 to CO 2 approximated to − 20 kJ mol − 1 when acetate concentrations were low, indicating that acetogens may have contributed to the flow of carbon and reductant towards methane.

show abstract

“…Cells were permeabilized in the respective assay cuvettes with cetyltrimethyl ammonium bromide (CTAB; details see Schink et al 1987). Hydrogenase, acetaldehyde: ferredoxin oxidoreductase, and pyruvate: ferredoxin oxidoreductase were measured with benzylviologen as electron acceptor (Schink 1985;Odom & Peck 1981), phosphotransacetylase and acetate kinase after Bergmeyer (1974), malate dehydrogenase and fumarase after Stams et al (1984), succinate dehydrogenase after Boonstra et al (1975), propionyl CoA: succinate CoA transferase after Hilpert et al (1984), lactate dehydrogenase after Bergmeyer (1974) and Stams & Hansen (1982).…”

Section: Enzymesmentioning

confidence: 99%

Fermentative degradation of dipicolinic acid (pyridine-2,6-dicarboxylic acid) by a defined coculture of strictly anaerobic bacteria

Seyfried

Schink

1990

Biodegradation

View full text Add to dashboard Cite

Degradation of dipicolinic acid (pyridine-2,6-dicarboxylic acid) under strictly anaerobic conditions was studied in enrichment cultures from marine and freshwater sediments. In all cases, dipicolinic acid was completely degraded. From an enrichment culture from a marine sediment, a defined coculture of two bacteria was isolated. The dipicolinic acid-fermenting bacterium was a Gram-negative, non-sporeforming strictly anaerobic short rod which utilized dipicolinic acid as sole source of carbon, energy, and nitrogen, and fermented it to acetate, propionate, ammonia, and 2CO2. No other substrate was fermented. This bacterium could be cultivated only in coculture with another Gram-negative, non-sporeforming rod from the same enrichment culture which oxidized acetate to CO2 with fumarate, malate, or elemental sulfur as electron acceptor, similar to Desulfuromonas acetoxidans. Since this metabolic activity is not important in substrate degradation by the coculture, the basis of the dependence of the dipicolinic acid-degrading bacterium on the sulfur reducer may be sought in the assimilatory metabolism.

show abstract

Pathway of propionate formation from ethanol in Pelobacter propionicus

Cited by 65 publications

References 40 publications

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Energetics and kinetics of lactate fermentation to acetate and propionate via methylmalonyl-CoA or acrylyl-CoA

Peat: home to novel syntrophic species that feed acetate- and hydrogen-scavenging methanogens

Fermentative degradation of dipicolinic acid (pyridine-2,6-dicarboxylic acid) by a defined coculture of strictly anaerobic bacteria

Contact Info

Product

Resources

About