Pathway engineering via quorum sensing and sRNA riboregulators—Interconnected networks and controllers

Carter, Karen K.; Valdés, James J.; Bentley, William E.

doi:10.1016/j.ymben.2011.11.006

Cited by 19 publications

(19 citation statements)

References 119 publications

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“…Cells have many natural mechanisms to sense and respond to their surroundings, and dynamic metabolic engineering has begun to harness this cellular machinery for use in controlled autonomous regulation (Carter et al, 2012). One commonly used mechanism is quorum sensing, the process through which cells secrete and sense autoinducers to sense their population density.…”

Section: Sensory Mechanisms To Determine the State Of The Cellmentioning

confidence: 99%

Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems

McNerney

Watstein

Styczynski

2015

Metabolic Engineering

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Metabolic engineering is generally focused on static optimization of cells to maximize production of a desired product, though recently dynamic metabolic engineering has explored how metabolic programs can be varied over time to improve titer. However, these are not the only types of applications where metabolic engineering could make a significant impact. Here, we discuss a new conceptual framework, termed “precision metabolic engineering,” involving the design and engineering of systems that make different products in response to different signals. Rather than focusing on maximizing titer, these types of applications typically have three hallmarks: sensing signals that determine the desired metabolic target, completely directing metabolic flux in response to those signals, and producing sharp responses at specific signal thresholds. In this review, we will first discuss and provide examples of precision metabolic engineering. We will then discuss each of these hallmarks and identify which existing metabolic engineering methods can be applied to accomplish those tasks, as well as some of their shortcomings. Ultimately, precise control of metabolic systems has the potential to enable a host of new metabolic engineering and synthetic biology applications for any problem where flexibility of response to an external signal could be useful.

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Section: Sensory Mechanisms To Determine the State Of The Cellmentioning

confidence: 99%

Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems

McNerney

Watstein

Styczynski

2015

Metabolic Engineering

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“…Advanced genetic devices developed by synthetic biologists have shown a wide range of programmability of cellular and molecular dynamics and have started to be increasingly exploited for metabolic engineering (Carter et al, 2012;Bacchus and Fussenegger, 2013).…”

Section: Discussionmentioning

confidence: 99%

Autonomous production of 1,4-butanediol via a de novo biosynthesis pathway in engineered Escherichia coli

Liu

2015

Metabolic Engineering

103

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“…[ 30 ] AHLs are the signaling molecules that consist of a homoserine lactone ring linked to a fatty acyl side chain varying from 4 to 18 carbon atoms ( Table 1 ). LuxI‐LuxR type QS systems, such as LuxI/LuxR in Vibrio fischeri , [ 31 ] LasI/LasR and RhlI/RhlR in Pseudomonas aeruginosa , CqsA/CqsS and LuxM/LuxN in V. harveyi , [ 32 ] and EsaI/EsaR in Pantoea stewartia , are the most well‐characterized AHLs‐mediated QS systems. In V. fischeri , the gene LuxI encodes AHL synthase that produces N ‐(3‐oxo‐hexanoyl)‐ l ‐homoserine lactone by condensation and lactonization of the substrates S ‐adenosylmethionine (SAM) and hexanoyl‐ACP.…”

Section: Quorum Sensing In Natural Cell Metabolismmentioning

confidence: 99%

Quorum Sensing System Used as a Tool in Metabolic Engineering

Sheng

Chen

et al. 2020

Biotechnology Journal

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Quorum sensing (QS) is a ubiquitous cell-cell communication mechanism in microbes that coordinates population-level cell behaviors, such as biofilm production, virulence, swarming motility, and bacterial persistence. Efforts to engineer QS systems to take part in metabolic network regulation represent a promising strategy for synthetic biology and pathway engineering. Recently, design, construction, and implementation of QS circuits for programmed control of bacterial phenotypes and metabolic pathways have gained much attention, but have not been reviewed recently. In this article, the architectural organizations and genetic contributions of the naturally occurring QS components to understand the mechanisms are summarized. Then, the most recent progress in application of QS toolkits to develop synthetic networks for novel cell behaviors creation and metabolic pathway engineering is highlighted. The current challenges in large-scale application of these QS circuits in synthetic biology and metabolic engineering fields are discussed and future perspectives for further engineering efforts are provided.

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Pathway engineering via quorum sensing and sRNA riboregulators—Interconnected networks and controllers

Cited by 19 publications

References 119 publications

Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems

Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems

Autonomous production of 1,4-butanediol via a de novo biosynthesis pathway in engineered Escherichia coli

Quorum Sensing System Used as a Tool in Metabolic Engineering

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