Pathotyping avian pathogenic<i>Escherichia coli</i>strains in Korea

Jeong, Yong Wun; Kim, Tae Eun; Kim, Jae Hong; Kwon, Hanjung

doi:10.4142/jvs.2012.13.2.145

Cited by 66 publications

(68 citation statements)

References 38 publications

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“…The frequency of vat has been reported as 10%, 34% and 95% [19,20,23]. In this work, vat was detected in 13% of the isolates and it is located in pathogenicity island (PAI).…”

Section: Discussionmentioning

confidence: 71%

“…Iron uptake appears to be one of the main virulence factors in strains of APEC. Jeong et al [23], attribute the presence of redundant iron uptake systems in APEC to the fact that these could function in different host's niches.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the number of virulence genes was similar in both groups with an average of seven genes, which is not in agreement with that described for group B1, which is mainly formed by commensal strains. Zhao et al [26], reported that 40% of APEC isolates analyzed belong to group A, which has also been classified as commensal strains and IPEC; likewise, Jeong et al [23] found high percentages of APEC isolates within groups A and B. Under this, it is assumed that the classification of E. coli in phylogenetic groups per se, does not allow differentiating the commensal strains of the ExPEC strains and suggests that the phylogenetic origin of the APEC isolates is diverse.…”

Section: Discussionmentioning

confidence: 99%

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Genes of Virulence and Phylogenetic Group in Isolates of Avian Pathogenic Escherichia coli

VHM¹,

IQ²,

PDPM³

et al. 2017

Arch Med

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Avian pathogenic Escherichia coli (APEC) shares some virulence attributes with strains of E. coli that cause extraintestinal infections in humans. The APEC is considered a possible cause for a zoonosis. The objective of this work was to determine the prevalence of twelve genes that are associated with virulence in a group of APEC isolates, as well as to identify the phylogenetic groups to which they belong. According to the results, one of these isolates harbors 91.6% of the virulence genes and most of the isolates have 7 or 8 of such twelve genes. The genes feoB and iss had the highest prevalence, with 95.6%. Genes associated to the acquisition of iron were present in more than 60% of the APEC isolates, while those of the ibeA invasin and vat toxin were detected with the lowest prevalence. A great genetic diversity was observed on the APEC isolates, which suggest that bacterial systems for iron acquisition, and those related to bacterial resistance to the host's defense mechanisms are fundamental virulence factors in these bacteria. On the other hand, the rest of the virulence genes provide valuable information for the development of vaccines against avian colibacillosis. It was also determined that a high percentage of APEC belongs to the phylogenetic group B1, from which mainly commensal and pathogenic E. coli strains derive.

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“…The frequency of vat has been reported as 10%, 34% and 95% [19,20,23]. In this work, vat was detected in 13% of the isolates and it is located in pathogenicity island (PAI).…”

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genes of Virulence and Phylogenetic Group in Isolates of Avian Pathogenic Escherichia coli

VHM¹,

IQ²,

PDPM³

et al. 2017

Arch Med

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“…A number of studies have demonstrated an association between the presence of these VGs and APEC pathogenicity (Janssen et al, 2001;Jeong et al, 2012;Mokady et al, 2005;Paixao et al, 2016). …”

Section: Yersiniabactinmentioning

confidence: 99%

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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“…Due to increased resistance to antibiotics and the risk of drug residues in poultry production, attention is more focused on vaccine development. This goal requires characterization of major and common virulence genes and determine their abundance in each geographic location (Kwaga et al 1994, Jeong et al 2012. Full understanding and use of the unique characteristics of APEC as targets for vaccination or diagnosis result in the planning criteria in order to control colibacillosis effectively (Boerlin andWhite 2006, Moulin-Schouleur et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Frequency of iss and irp2 genes by PCR method in Escherichia coli isolated from poultry with colibacillosis in comparison with healthy chicken in poultry farms of Zabol, South East of Iran

Bonjar¹,

Salari²,

Jahantigh³

et al. 2017

Polish Journal of Veterinary Sciences

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There is no special trait for differentiation of Avian Pathogenic Escherichia coli from Avian Fecal Escherichia coli. This investigation is aimed, as a case control study, to evaluate and compare the frequency of iss and irp2 in 43 AFEC strains and also 40 and 56 E. coli strains isolated from the liver and kidney of chickens with colibacillosis, respectively, farmed in Zabol, as a border region of Iran, by PCR. 86.9% and 37.2% of isolates collected from chickens with colibacillosis and feces samples obtained from healthy chickens were positive for iss gene, respectively (P<0.05). On average, 59.3% of E. coli strains isolated from colibacillosis have irp2 gene while 27.9% of isolates from the feces of healthy birds were positive (P<0.05). 52.15% of isolates from colibacillosis and 19.62% of isolates from healthy chicken feces were positive for both genes, with statistical significant difference (p<0.05). This marked difference in the distribution of iss and irp2 genes makes these two genes good markers to differentiate AFEC and APEC strains especially in Sistan region to improve colibacillosis control measurements.

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Pathotyping avian pathogenicEscherichia colistrains in Korea

Cited by 66 publications

References 38 publications

Genes of Virulence and Phylogenetic Group in Isolates of Avian Pathogenic Escherichia coli

Genes of Virulence and Phylogenetic Group in Isolates of Avian Pathogenic Escherichia coli

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Frequency of iss and irp2 genes by PCR method in Escherichia coli isolated from poultry with colibacillosis in comparison with healthy chicken in poultry farms of Zabol, South East of Iran

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