Pathotype Separation of <i>Plasmodiophora brassicae</i> by the Host Plant

Fähling, Michael; Graf, H.; Siemens, Johannes

doi:10.1046/j.1439-0434.2003.00744.x

Cited by 81 publications

(67 citation statements)

References 23 publications

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“…The selection isolate eH (Fähling et al 2003), belonging to pathotype 1, was used in all experiments. The isolate is maintained on roots of the universal susceptible host Chinese cabbage (B. rapa subsp.…”

Section: Plant and Pathogen Materialmentioning

confidence: 99%

Metabotyping: A New Approach to Investigate Rapeseed (Brassica napus L.) Genetic Diversity in the Metabolic Response to Clubroot Infection

Wagner¹,

Charton²,

Lariagon³

et al. 2012

MPMI

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Clubroot disease affects all Brassicaceae spp. and is caused by the obligate biotroph pathogen Plasmodiophora brassicae. The development of galls on the root system is associated with the establishment of a new carbon metabolic sink. Here, we aimed to deepen our knowledge of the involvement of primary metabolism in the Brassica napus response to clubroot infection. We studied the dynamics and the diversity of the metabolic responses to the infection. Root system metabotyping was carried out for 18 rapeseed genotypes displaying different degrees of symptom severity, under inoculated and noninoculated conditions at 42 days postinoculation (dpi). Clubroot susceptibility was positively correlated with clubroot-induced accumulation of several amino acids. Although glucose and fructose accumulated in some genotypes with minor symptoms, their levels were negatively correlated to the disease index across the whole set of genotypes. The dynamics of the metabolic response were studied for the susceptible genotype 'Yudal,' which allowed an "early" metabolic response (established from 14 to 28 dpi) to be differentiated from a "late" response (from 35 dpi). We discuss the early accumulation of amino acids in the context of the establishment of a nitrogen metabolic sink and the hypothetical biological role of the accumulation of glutathione and S-methylcysteine.

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Section: Plant and Pathogen Materialmentioning

confidence: 99%

Metabotyping: A New Approach to Investigate Rapeseed (Brassica napus L.) Genetic Diversity in the Metabolic Response to Clubroot Infection

Wagner¹,

Charton²,

Lariagon³

et al. 2012

MPMI

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“…Field populations of P. brassicae are heterogenic, and therefore introducing CR cultivars into infested soil has been considered to result in the appearance of highly virulent races through selection from the populations (Seaman et al 1963;Jones et al 1982;Kageyama et al 1995;Fähling et al 2003). In this work, races that were highly virulent to CR cultivars were not detected from the SSGs.…”

Section: Pathogenicity Of Populations Isolated From Ssgsmentioning

confidence: 82%

Pathogenicity of Plasmodiophora brassicae populations from small, spheroid, resistant-type clubroot galls on roots of clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. subsp. pekinensis)

2008

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A resistant type of small, spheroid clubroot galls (SSGs) containing resting spores formed on the root surface of clubroot-resistant (CR) cultivars of Chinese cabbage (Brassica rapa L. subsp. pekinensis) inoculated with an avirulent population of Plasmodiophora brassicae. Populations isolated from the SSGs severely affected a common (susceptible) cultivar but did not have the typical pathogenicity on CR cultivars, indicating similarity in pathogenicity between SSG and original spore populaions. Populations pathogenic on CR cultivars were not detected from SSGs. Therefore, the ability of the avirulent population among the SSGs to form resting spores may need to be considered to achieve clubroot control in common cultivars of crucifers.

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“…Analysis of the relation between the diversity of pathogenicity (races and pathotypes) and DNA polymorphisms in P. brassicae populations has been tried by some workers (Buhariwalla et al 1995;Möller and Harling 1996;Yano et al 1997;Manzanares-Dauleux et al 2001;Fähling et al 2003). Yano et al (1997) reported that RAPD analysis was able to clearly differentiate Williams' race 9 populations from other race populations (races 1 and 4) but unable to differentiate virulent populations from avirulent ones on CR cultivars of Chinese cabbage bred in Japan.…”

Section: Discussionmentioning

confidence: 99%

Relation between pathogenicity and genetic variation within Plasmodiophora brassicae

et al. 2008

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The relation between diversity of pathogenicity on clubroot-resistant (CR) cultivars of Chinese cabbage (Brassica rapa subsp. pekinensis) bred in Japan and DNA polymorphisms in 17 populations of Plasmodiophora brassicae from cruciferous plants was examined by inoculation tests and random amplified polymorphic DNA (RAPD) analysis using 18 arbitrary primers. Four pathotypes (A-D) were identified after inoculation of six CR cultivars of Chinese cabbage in the 17 populations from cruciferous crops. A relatively high level of genetic diversity was also detected among these populations in the RAPD analysis. Although the four pathotypes could not be clearly differentiated using the RAPD data, most populations of three pathotypes had a consistent location on the dendrogram. All pathotype B (virulent on five cultivars except Utage 70) and D (avirulent on all cultivars) populations, which were common in incompatible interactions with cv. Utage 70, were located in a single subcluster. All five pathotype C populations (virulent only on cv. Utage 70) except for one population grouped in another single subcluster. Because four pathotype A populations (virulent on all six cultivars, races 4 and 9) fell in different subclusters, the populations may be genetically polyphyletic. Populations from cruciferous weed Cardamine flexuosa differed remarkably from those from cruciferous crops in pathogenicity on common cultivars of Chinese cabbage and turnip and C. flexuosa, but they grouped in a single cluster with all race 9 populations from crops. Race 9 populations from crops may thus be closely related to populations from the weed rather than to races 1 and 4 from crops.

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Pathotype Separation of Plasmodiophora brassicae by the Host Plant

Cited by 81 publications

References 23 publications

Metabotyping: A New Approach to Investigate Rapeseed (Brassica napus L.) Genetic Diversity in the Metabolic Response to Clubroot Infection

Metabotyping: A New Approach to Investigate Rapeseed (Brassica napus L.) Genetic Diversity in the Metabolic Response to Clubroot Infection

Pathogenicity of Plasmodiophora brassicae populations from small, spheroid, resistant-type clubroot galls on roots of clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. subsp. pekinensis)

Relation between pathogenicity and genetic variation within Plasmodiophora brassicae

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