Pathophysiological Evaluation of the Protective Role of Alpha Lipoic Acid on Diabetes Mellitus Nephropathy in Male Rabbits

Abstract

In order to estimate the ameliorating effects of alpha lipoic acid (ALA) on the nephropathy of diabetes mellitus (DM) associated with oxidative stress, this study performed in 18 adults male rabbits were divided into 3 equal groups, the group-I was regard as control while group-II Induced DM only by intravenously single dose (150 mg/kg b.w.) of alloxan-monohydrate; group-III was induced DM by intravenously single dose of (150mg/kg b.w.) of alloxan-monohydrate and then treated by (10mg/kg b.w.) daily of ALA intraperitoneally for 4 weeks. The biochemical results showed significant (P≤0.05) decreased of the serum values of GSH, CAT and SOD as well, significantly (P≤0.05) increased of MDA, peroxynitrate, creatinine and BUN of group-II when compared to control; also the result of group-III showed nonsignificant (P˃0.05) differences of GSH, SOD, MDA, creatinine, BUN and peroxynitrate when compared to control. The histopathological and histochemical results of kidney of group-II showed moderate thickness of basement membrane of glomeruli with compressed capillaries as well infiltration of glyco-proteinaceous materials in glomeruli and around renal tubules with some vacuolation of these tubules and mesangial cells; while the results of group-III showed normal architectures with very mild degree of vacuolation of few renal tubules in addition to disappearance of glyco-proteinaceous materials in this group; in conclusion, ALA had a dual protective effects on nephropathy by scavenging the oxidative stress free radicals and enhanced insulin metabolism.

Medical Ozone Treatment Alleviate Blood Oxidative Stress And Pancreas Damage In an Alloxan-Induced Diabetes Model in Rats

“…Glucose auto-oxidation and protein glycation are important additional sources of free radicals during hyperglycemia [39]. [40,41] reported a decrease in total antioxidant status in diabetes mellitus, thus indicating the delicate balance between oxidants and antioxidants, whereas hyperglycemia can result in the generation of free radicals through several biochemical pathways such as nonenzymatic glycation, the poly pathway, and glucose auto-oxidation [42]. Free radicals can result in the destruction of antioxidant defences and enhanced susceptibility to lipid peroxidation.…”