Pathophysiological dual action of adiponectin after transient focal ischemia in mouse brain

Yatomi, Kenji; Miyamoto, Nobukazu; Komine-Kobayashi, Miki; Liu, Meizi; Oishi, Hidenori; Arai, Hajime; Urabe, Takao

doi:10.1016/j.brainres.2009.08.039

Cited by 22 publications

(13 citation statements)

References 37 publications

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“…Therefore, we hypothesize that increased TNF-α expression might be one of the mechanisms responsible for the decrease in the plasma adiponectin level after TBI that was observed in the present study. Several authors have reported a high level of adiponectin immunoreactivity in the ischemic hemisphere in mice (20,24), whereas adiponectin mRNA was not detected in the brain (24). Taken together with these past results, our data suggest that the immunoreactivity of adiponectin in the brain reflects not the production, but the accumulation, in the injured brain (24).…”

Section: Referencessupporting

confidence: 81%

“…The relationship between adiponectin and its signal after TBI, and the effects of adiponectin administration after TBI should be the subject of future investigations. reported that, in a mouse model of ischemia-reperfusion, the plasma adiponectin level decreased significantly until 48 h after reperfusion (24). Marousi et al reported that the serum adiponectin level was already significantly suppressed during the early phases of stroke, and that the levels remained unchanged 6 months later in humans (10).…”

Section: Discussionmentioning

confidence: 99%

“…It has recently been reported that the plasma adiponectin levels in patients with several acute illness decreased periodically, thus indicating that adiponectin may play a role in the inflammatory response in patients with acute illness (21). Furthermore, recent experimental studies using a cerebral ischemia-reperfusion model have shown that high levels of adiponectin were detected in the ischemic hemisphere (24), and that adiponectin may exert a cerebroprotective action (13). The precise mechanism(s) underlying its effects remains elusive, but it has been reported that adiponectin exerts its effect mainly by activating the AMPK and PPARα pathways (9, 13,22,23).…”

Section: Western Blot Analysismentioning

confidence: 99%

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Decrease in plasma adiponectin level and increase in adiponectin immunoreactivity in cortex and hippocampus after traumatic brain injury in rats

Takeuchi

Wada²,

Nawashiro³

et al. 2012

Turkish Neurosurgery

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AIm: Adiponectin plays an important role in the regulation of tissue inflammation. Recently, it has been reported that the plasma adiponectin levels in several acute illnesses decrease periodically, thus indicating that adiponectin may play a role in the inflammatory response in patients with acute illness. However, little is known about the effects of adiponectin following TBI. The aim of the present study was to examine the changes in the plasma adiponectin levels and the immunoreactivity of adiponectin in the brain after TBI. mAterIAl and methOds: Adult male Sprague-Dawley rats were subjected to lateral fluid percussion injury using the Dragonfly device. Plasma adiponectin levels were determined by ELISA kit. Immunohistochemistry and Western blot analysis were performed to assess the immunoreactivity of adiponectin. results:The plasma adiponectin levels gradually decreased and were significantly lower at 48 h and 72h after injury than before injury. Immunohistochemistry and Western blot analysis showed that the adiponectin immunoreactivity was increased in the cerebral cortex at 24 hours after injury and in the hippocampus at 72 hours after injury.COnClusIOn: Our findings suggest that adiponectin might participate in the pathophysiological process occurring after TBI.KeywOrds: Adiponectin, Traumatic brain injury, Lateral fluid percussion, Rat ÖZ AmAÇ: Adiponektin doku enflamasyonunun düzenlenmesinde önemli bir rol oynar. Son zamanlarda plazma adiponektin düzeylerinin çeşitli akut hastalıklarda düzenli olarak azalması nedeniyle adiponektinin akut hastalığı olan hastalarda enflamatuvar cevapta bir rol oynayabileceği bildirilmiştir. Ancak travmatik beyin hasarı sonrasında adiponektin etkileri hakkında çok az şey bilinmektedir. Mevcut çalışmanın amacı travmatik beyin hasarı sonrasında plazma adiponektin düzeylerinin değişiklikleri ve beyinde adiponektin immünoreaktivitesini incelemekti. yÖntem ve GereÇler: Yetişkin erkek Sprague-Dawley sıçanlarında Dragonfly cihazları kullanılarak lateral sıvı perküsyon hasarı oluşturuldu. Plazma adiponektin düzeyleri ELISA kitiyle belirlendi. Adiponektin immünoreaktivitesini değerlendirmek üzere immünohistokimya ve Western blot analizi yapıldı.BulGulAr: Plazma adiponektin düzeyleri giderek azaldı ve yaralanmadan 48 saat ve 72 saat sonra yaralanma öncesine göre önemli ölçüde daha düşüktü. İmmünohistokimya ve Western blot analizi adiponektin immünoreaktivitesinin serebral kortekste yaralanmadan 24 saat sonra ve hippocampuste yaralanmadan 72 saat sonra arttığını gösterdi. sOnuÇ: Bulgularımız adiponektinin travmatik beyin hasarı sonrasındaki patofizyolojik sürece katkıda bulunabileceğini düşündürmektedir.

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Section: Referencessupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

See 1 more Smart Citation

Decrease in plasma adiponectin level and increase in adiponectin immunoreactivity in cortex and hippocampus after traumatic brain injury in rats

Takeuchi

Wada²,

Nawashiro³

et al. 2012

Turkish Neurosurgery

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“…Evidence suggests that ischemia/reperfusion injury leads to increased adiponectin expression in brain vessels, 31 a vascular bed where uncoupled eNOS is a key contributor to vascular O 2 − generation. 32 Moreover, it has recently been shown that 4-HNE, a product of peroxidation released from tissues with high oxidative burden, upregulates ADIPOQ in primary human and 3T3 adipocytes, 18 as well as in skeletal muscle.…”

Section: Effects Of Vascular Oxidative Stress On Adiponectin Expressimentioning

confidence: 99%

Interactions Between Vascular Wall and Perivascular Adipose Tissue Reveal Novel Roles for Adiponectin in the Regulation of Endothelial Nitric Oxide Synthase Function in Human Vessels

et al. 2013

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Background-Adiponectin is an adipokine with potentially important roles in human cardiovascular disease states.We studied the role of adiponectin in the cross-talk between adipose tissue and vascular redox state in patients with atherosclerosis. Methods and Results-The study included 677 patients undergoing coronary artery bypass graft surgery. Endothelial function was evaluated by flow-mediated dilation of the brachial artery in vivo and by vasomotor studies in saphenous vein segments ex vivo. Vascular superoxide (O 2 − ) and endothelial nitric oxide synthase (eNOS) uncoupling were quantified in saphenous vein and internal mammary artery segments. Local adiponectin gene expression and ex vivo release were quantified in perivascular (saphenous vein and internal mammary artery) subcutaneous and mesothoracic adipose tissue from 248 patients. Circulating adiponectin was independently associated with nitric oxide bioavailability and O 2 − production/ eNOS uncoupling in both arteries and veins. These findings were supported by a similar association between functional polymorphisms in the adiponectin gene and vascular redox state. In contrast, local adiponectin gene expression/release in perivascular adipose tissue was positively correlated with O 2 − and eNOS uncoupling in the underlying vessels. In ex vivo experiments with human saphenous veins and internal mammary arteries, adiponectin induced Akt-mediated eNOS phosphorylation and increased tetrahydrobiopterin bioavailability, improving eNOS coupling. In ex vivo experiments with human saphenous veins/internal mammary arteries and adipose tissue, we demonstrated that peroxidation products produced in the vascular wall (ie, 4-hydroxynonenal) upregulate adiponectin gene expression in perivascular adipose tissue via a peroxisome proliferator-activated receptor-γ-dependent mechanism. Conclusions-We demonstrate for the first time that adiponectin improves the redox state in human vessels by restoring eNOS coupling, and we identify a novel role of vascular oxidative stress in the regulation of adiponectin expression in human perivascular adipose tissue. (Circulation. 2013;127:2209-2221.)

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“…The close correlation between adiponectin and ischemic stroke has been confirmed in numerous studies (Yatomi et al, 2009;Marousi et al, 2010;Nagasawa et al, 2011). In addition, polymorphisms in the adiponectin gene may affect adiponectin levels in the serum (Mente et al, 2013).…”

Section: Introductionmentioning

confidence: 72%

Association between polymorphisms in the adiponectin gene (APM-1) and atherosclerotic cerebral infarction in a Hainan Chinese Han population

Chen

Ding

2015

Genet. Mol. Res.

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ABSTRACT. We investigated the association between polymorphisms in the adiponectin gene (APM-1) and atherosclerotic cerebral infarction (ACI) in a Chinese Han population of Hainan Province. Polymerase chain reaction-restriction fragment length polymorphism and gene sequencing were used to analyze the distribution of APM-1 +45T/G and +276G/T genotypes and their alleles in 120 ACI patients and 120 healthy controls. No statistical correlation was found in the frequency and distribution of the genotype 45T/G between the ACI group and the control group. Genotypic frequencies of GG, GT, and TT at the APM-1 +276 locus were 70.0% (84/120), 25.0% (30/120), and 5.0% (6/120), respectively, in the ACI group, while these values were 52.5% (63/120), 37.5% (45/120), and 10.0% (12/120), respectively, in the control group. The frequency of the G allele was 82.5% (198/240) in the ACI group and 71.25% (171/240) in the control group. The T allele frequency was 17.5% (42/240) in the ACI group and 28.75% (69/240) in the control group. Polymorphisms at the APM-1 -276 locus in the case-controlled 4143 Associations between polymorphisms in APM-1 gene and ACI ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 4142-4149 (2015) groups showed significant differences in the genotype distribution and allele frequency between the 2 groups (P = 0.041). The occurrence of ACI in the Hainan Chinese Han population may be associated with +276G/T polymorphisms but not with +45T/G polymorphisms in the APM-1 gene.

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Pathophysiological dual action of adiponectin after transient focal ischemia in mouse brain

Cited by 22 publications

References 37 publications

Decrease in plasma adiponectin level and increase in adiponectin immunoreactivity in cortex and hippocampus after traumatic brain injury in rats

Decrease in plasma adiponectin level and increase in adiponectin immunoreactivity in cortex and hippocampus after traumatic brain injury in rats

Interactions Between Vascular Wall and Perivascular Adipose Tissue Reveal Novel Roles for Adiponectin in the Regulation of Endothelial Nitric Oxide Synthase Function in Human Vessels

Association between polymorphisms in the adiponectin gene (APM-1) and atherosclerotic cerebral infarction in a Hainan Chinese Han population

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