Pathophysiological aspects of Mecistocirrus digitatus (Nematoda: Trichostrongylidae) infection in calves

Aken, D. Van; Vercruysse, Jozef; Dargantes, A.P.; Lagapa, Jose Trinipil; Raes, Stefaan; Shaw, Darren

doi:10.1016/s0304-4017(96)01132-6

Cited by 16 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Given that anaemia and a reduction in weight gain caused by the haematophagous activity of adult stages seem to be the most important pathogenic effects of M. digitatus infection in calves and are similar to those observed during infection with Haemonchus placei in calves and H. contortus in sheep and goats [2], [18], we sought to compare native protein vaccine preparations, enriched for intestinal surface proteins by Concanavalin A lectin affinity binding [19] from H. contortus and M. digitatus using proteomics, and to evaluate the protective efficacy of the latter against H. contortus challenge in sheep as a prelude to vaccine trials in buffalo in India. Cross-protection has been previously shown to occur in trials conducted in sheep which had been immunized with native Ostertagia protein fractions but challenged with H. contortus ; the Ostertagia antigens cross-protected efficiently against Haemonchus [20], as such cross-protection between M. digitatus and H. contortus was believed to be likely.…”

Section: Introductionmentioning

confidence: 69%

See 1 more Smart Citation

Proteomic Analysis of Mecistocirrus digitatus and Haemonchus contortus Intestinal Protein Extracts and Subsequent Efficacy Testing in a Vaccine Trial

et al. 2014

View full text Add to dashboard Cite

BackgroundGastrointestinal nematode infections, such as Haemonchus contortus and Mecistocirrus digitatus, are ranked in the top twenty diseases affecting small-holder farmers' livestock, yet research into M. digitatus, which infects cattle and buffalo in Asia is limited. Intestine-derived native protein vaccines are effective against Haemonchus, yet the protective efficacy of intestine-derived M. digitatus proteins has yet to be determined.Methodology/Principal FindingsA simplified protein extraction protocol (A) is described and compared to an established method (B) for protein extraction from H. contortus. Proteomic analysis of the H. contortus and M. digitatus protein extracts identified putative vaccine antigens including aminopeptidases (H11), zinc metallopeptidases, glutamate dehydrogenase, and apical gut membrane polyproteins. A vaccine trial compared the ability of the M. digitatus extract and two different H. contortus extracts to protect sheep against H. contortus challenge. Both Haemonchus fractions (A and B) were highly effective, reducing cumulative Faecal Egg Counts (FEC) by 99.19% and 99.89% and total worm burdens by 87.28% and 93.64% respectively, compared to the unvaccinated controls. There was no effect on H. contortus worm burdens following vaccination with the M. digitatus extract and the 28.2% reduction in cumulative FEC was not statistically significant. However, FEC were consistently lower in the M. digitatus extract vaccinates compared to the un-vaccinated controls from 25 days post-infection.Conclusions/SignificanceSimilar, antigenically cross-reactive proteins are found in H. contortus and M. digitatus; this is the first step towards developing a multivalent native vaccine against Haemonchus species and M. digitatus. The simplified protein extraction method could form the basis for a locally produced vaccine against H. contortus and, possibly M. digitatus, in regions where effective cold chains for vaccine distribution are limited. The application of such a vaccine in these regions would reduce the need for anthelmintic treatment and the resultant selection for anthelmintic resistant parasites.

show abstract

Section: Introductionmentioning

confidence: 69%

“…Infections with blood-feeding gastrointestinal nematodes, such as Haemonchus contortus and Mecistocirrus digitatus , cause significant animal welfare and production losses globally [1], [2]. The latter is an important blood-sucking nematode of cattle in Asia and Central America [3].…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Mecistocirrus digitatus and Haemonchus contortus Intestinal Protein Extracts and Subsequent Efficacy Testing in a Vaccine Trial

et al. 2014

View full text Add to dashboard Cite

show abstract

“…which exhibited a clear dose-dependent relationship. Likewise, similar parasite burdens were found after single and trickle infections with Teladorsagia circumcincta (Gruner et al 1994), Taenia asiatica (Fall et al 1996), Mecistocirrus digitatus (Van Aken et al 1997), Oesophagostomum dentatum (Barnes 1997) and Fasciola hepatica (Bossaert et al 2000a, b). However, despite similar burdens, mode of infection has been found to have an effect on parasite development and/or fecundity, which is suggestive of competition and/or immune effects.…”

Section: Experimental Trickle and Single Infectionsmentioning

confidence: 84%

“…For instance, sheep have been experimentally exposed to single and trickle infections with the nematodes Haemonchus contortus (Barger et al 1985, McClure et al 1998, Trichostrongylus colubriformis (Dobson et al 1990, McClure et al 1998, and Teladorsagia circumcincta (Gruner et al 1994); pigs to the nematode Oesophagostomum dentatum (Barnes 1997, Talvik et al 1999 or Taenia asiatica (Fall et al 1996); and calves to Cooperia oncophora (Van Diemen et al 1996), Mecistocirrus digitatus (Van Aken et al 1997) and Fasciola hepatica (Bossaert et al 2000a, b). A common feature of these studies is that parasite burdens reached by either single or trickle infection are comparable.…”

mentioning

confidence: 99%

Trickle and single infection with Discocotyle sagittata (Monogenea: Polyopisthocotylea): effect of exposure mode on parasite abundance and development

Rubio‐Godoy¹,

Tinsley²

2002

FOLIA PARASIT

View full text Add to dashboard Cite

Abstract. Experimental infection of rainbow trout Oncorhynchus mykiss (Walbaum) with the monogenean Discocotyle sagittata (Leuckart, 1842) allowed comparison between trickle and single exposure, two infection modes demonstrated to occur in the wild. Both types of infection resulted in mean larval attachment success around 50%, which was significantly dependent on dose of infective larvae used (P < 0.0001), but was not affected by mode of infection (P = 0.244). Worms recovered from fish exposed to the same number of oncomiracidia but different mode of infection differed in their rate of development. The developmental stage attained by parasites was significantly affected by number of infective larvae used (P = 0.005), and by the interaction between dose and mode of infection (P = 0.026), suggesting competition among attached larvae. Statistical analysis demonstrated that in the early stages of infestation, worm distribution over the gill arches can be explained by the relative amount of water flowing over them. One, two and three months post-infection parasite numbers were comparable (P = 0.805), but their observed distribution gradually decreased in gill arches III and IV and increased in gill arch I, suggesting that parasites migrate after initial attachment. These results reproduce phenomena observed in the field, indicating that the experimental infection system could be employed to study infection dynamics and host-parasite interactions under controlled conditions.

show abstract

“…This parasite may cause mucosal inflammation, haemorrhage, ulcers and necrosis in the abomasums. It appears to be confined mainly to Asian countries; however, it has also been found in Central America, Egypt and Russia [6,7,13,21,22]. The movement of its hosts for agricultural purposes has resulted in the global spread of this parasite.…”

mentioning

confidence: 99%

PCR-based Species-Specific Amplification of ITS of Mecistocirrus digitatus and Its Application in Identification of GI Nematode Eggs in Bovine Faeces

et al. 2006

View full text Add to dashboard Cite

ABSTRACT. Differential diagnosis of Mecistocirrus digitatus infection relies on morphological examination of either eggs in faecal samples or L3 larvae developed in vitro. Technical limitations hinder the practicability of these approaches. Hence, in order to develop a specific diagnostic measure for M. digitatus infection, we determined the sequence of the internal transcribed spacer (ITS) of its ribosomal DNA (rDNA) and designed primers for PCR-based species-specific amplification of the ITS to differentiate between M. digitatus and other common gastrointestinal (GI) nematode species. The newly designed primers amplified a single specific 520 base pair (bp) fragment from the M. digitatus ITS, and its detection limit was as low as 0.001 ng. Further, this sensitivity suggested that the specific fragment could be amplified even from a unicellular egg that collected directly from uteri of an adult M. digitatus female. In fact, we designed a method that employs a small piece of a cover slip and a filter paper by which we could differentially amplify a PCR fragment from a unicellular egg. The reliability of the specific PCR assay was also demonstrated with 10 oval samples that collected from bovine faeces by using sugar flotation method. These data suggested that the specific PCR assay of the ITS region of M. digitatus rDNA could be useful for the identification of GI nematodes. Gastrointestinal (GI) nematodes of domestic ruminants are of major veterinary importance due to their high prevalence, ubiquitous distribution and the pathological consequences of infection and the associated economic production losses [1,3,18]. In Japan, the major GI nematode species in cattle are Ostertagia ostertagi, Mecistocirrus digitatus and Trichostrongylus axei which are found in the abomasum; Strongyloides papillosus and Cooperia and Nematodirus species which are found in the small intestine; and Oesophagostomum radiatum found in the large intestine [7,15,16,23]. Of these species, M. digitatus, O. ostertagi and Cooperia oncophora are considered the most prevalent in cattle; in particular, the infection rate of M. digitatus was high at approximately 30-50 % [7,16]. This nematode is commonly described as a large stomach worm, namely, a trichostrongylid nematode, and is an important blood-sucking nematode present in the abomasums of cattle. This parasite may cause mucosal inflammation, haemorrhage, ulcers and necrosis in the abomasums. It appears to be confined mainly to Asian countries; however, it has also been found in Central America, Egypt and Russia [6,7,13,21,22]. The movement of its hosts for agricultural purposes has resulted in the global spread of this parasite.Generally, ruminants are concurrently infected with more than one species of GI nematodes, each having a different pathological effect on the host. It has been a difficult task to eradicate GI nematodes from grazing ruminants due to the variation in host susceptibility to the parasite, the wide distribution of nematodes in nature and the presence of wild ruminants. An...

show abstract

Pathophysiological aspects of Mecistocirrus digitatus (Nematoda: Trichostrongylidae) infection in calves

Cited by 16 publications

References 10 publications

Proteomic Analysis of Mecistocirrus digitatus and Haemonchus contortus Intestinal Protein Extracts and Subsequent Efficacy Testing in a Vaccine Trial

Proteomic Analysis of Mecistocirrus digitatus and Haemonchus contortus Intestinal Protein Extracts and Subsequent Efficacy Testing in a Vaccine Trial

Trickle and single infection with Discocotyle sagittata (Monogenea: Polyopisthocotylea): effect of exposure mode on parasite abundance and development

PCR-based Species-Specific Amplification of ITS of Mecistocirrus digitatus and Its Application in Identification of GI Nematode Eggs in Bovine Faeces

Contact Info

Product

Resources

About