Pathomorphism of Limb Major Vessels in Experimental Atherogenic Inflammation. The Role of Adventitial Intimal Relations (Review)

Кириченко, А. К.; Patlataya, Nadezhda N.; Шаркова, А Ф; Pevnev, A.A.; Kontorev, K.V.; Шаповалова, Ольга Владимировна; Gorban, M.E.; Bolshakov, I. N.

doi:10.17691/stm2017.9.3.20

Cited by 5 publications

(6 citation statements)

References 40 publications

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“…The dislocation of sulfated chitosan near cell membranes in the subintimal region suggests its active interaction with cholesterol in macrophages, its binding to extracellular and intracellular cholesterol, and transport of its water-soluble electrolyte complex with lipid mass [61] into the para-adventitial region in the composition of chitosan nuclei [60]. It is assumed that the targeted transport of lipid fractions occurs due to an electrostatic gradient [64][65][66], which is caused by artificial minimally invasive dislocation of chitosan [47] in the fascial sheath of large vessels. Our study convincingly shows the local release of cholesterol and low-density lipoproteins from the arterial wall.…”

Section: Discussionmentioning

confidence: 99%

“…This flow outside the outer shell of the vessel can be provided by an electrostatic gradient, which is created due to the polycationic properties of chitosan [60,64]. Artificial minimally invasive dislocation of chitosan nanoparticles in the fascial sheath of the main vessels provides this gradient [65,66] and can be designed for the directed flow of the lipid mass towards the adventitia.…”

Section: Mechanisms Of Formation Of Vascular Atherosclerosis and Sign...mentioning

confidence: 99%

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Synthesis, Chemical and Biomedical Aspects of the Use of Sulfated Chitosan

et al. 2022

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This work is devoted to the chemical synthesis of sulfated chitosan and its experimental verification in an animal model of early atherosclerosis. The method of chitosan quaternization with sulfate-containing ingredients resulted in a product with a high content of sulfate groups. Implantation of this product into the fascial-muscular sheath of the main limb artery along the leg and thigh in rabbits led to the extraction of cholesterol from the subintimal region. Simplified methods for the chemical synthesis of quaternized sulfated chitosan and the use of these products in a model of experimental atherosclerosis made it possible to perform a comparative morphological analysis of the vascular walls of the experimental and control limbs under conditions of a long-term high-cholesterol diet. The sulfated chitosan samples after implantation were shown to change the morphological pattern of the intimal and middle membranes of the experimental limb artery. The implantation led to the degradation of soft plaques within 30 days after surgical intervention, which significantly increased collateral blood flow. The implantation of sulfated chitosan into the local area of the atherosclerotic lesions in the artery can regulate the cholesterol content in the vascular wall and destroy soft plaques in the subintimal region.

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Section: Discussionmentioning

confidence: 99%

Section: Mechanisms Of Formation Of Vascular Atherosclerosis and Sign...mentioning

confidence: 99%

Synthesis, Chemical and Biomedical Aspects of the Use of Sulfated Chitosan

et al. 2022

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“…It is important to emphasize the independent mechanism of therapeutic angiogenesis in the presence of highly deacetylated chitosan. The implantation of chitosan constructs in the tissue of an intact animal is accompanied by an 85-96% increase in the number of microvessels after 30 days of the post-implantation period [203]. Such a local angiogenic effect of chitosan hydrogels is confirmed by fundamental research when non-covalent and covalent copolymers containing chitosan and a sulfated ingredient, for example, heparin, are used in experiments.…”

Section: The Role Of Chitosan Substrates and Other Polysaccharides In Stimulating Osteogenesis And Angiogenesismentioning

confidence: 91%

“…No less important is the fact of stimulation of vascular neoplasm in the presence of only highly deacetylated chitosan polymer in tissues without preliminary inclusion of vascular formation and growth factors in its composition. It has been established that constructions based on liquid polysaccharides, for example, the sulfated form of chitosan, chitosan ascorbate, chitosan hydrochloride, the sodium salt of alginic acid, when introduced into the fascial sheaths of the neurovascular bundles [201][202][203] create the effect of therapeutic angiogenesis. The concept of the active inclusion of molecular markers of angiogenesis and further morphological restructuring of the qualitative and quantitative characteristics of the vessels consists in the artificial placement of chitosan and other biopolymers in the immediately affected area.…”

Section: The Role Of Chitosan Substrates and Other Polysaccharides In Stimulating Osteogenesis And Angiogenesismentioning

confidence: 99%

The Role of Modified Chitosan in Bone Engineering in Diabetes Mellitus: Analytical Review

Bolshakov¹,

AA²,

NN³

et al. 2021

Int J Dent Oral Health

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The review is devoted to the state of the problem during the reconstruction of bone defects in the maxillofacial region, topical issues of the mechanisms of biopolymers action, mainly chitosan, during stimulation of angiogenesis and osteogenesis under conditions of the formation of bone cavities of critical size in the maxillofacial area, as well as under conditions of the development of an inflammatory process with Mellitus diabetes. In the analysis of research papers, the international information bases Web of Science, Scopus, PMC free article, PubMed, Google Scholar were used, mainly over the past 12 years. The main emphasis in the scientific review is placed on the points of application of the chitosan biopolymer in the implementation of already known signaling pathways for the regulation of osteogenesis. Studies have shown that chitosan, as an independent polymer, and especially chitosan copolymers, play an important role in the regulation of osteoblastogenesis and angiogenesis, reducing the osteoclastic response, preventing osteomalacia of the alveolar ridge, accelerating the formation of a new well-vascularized bone.

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“…It is very important that early active osteogenesis occurs under conditions of an antiinflammatory response since the structure of chitosan forms electrostatic and concentration gradients for cells, metabolites, and water, which causes them to move toward the polymer. This reduces the degree of inflammation both at the site of polymer dislocation and in the peripheral zone [17]. An artificial structure based on natural polysaccharides and apatites can successfully solve this problem of regeneration of large bone defects with careful selection of a combination of polymers.…”

Section: Introductionmentioning

confidence: 99%

Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region

Patlataya,

Bolshakov,

Levenets

et al. 2023

Polymers

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A biomaterial is proposed for closing extensive bone defects in the maxillofacial region. The composition of the biomaterial includes high-molecular chitosan, chondroitin sulfate, hyaluronate, heparin, alginate, and inorganic nanostructured hydroxyapatite. The purpose of this study is to demonstrate morphological and histological early signs of reconstruction of a bone cavity of critical size. The studies were carried out on 84 white female rats weighing 200–250 g. The study group consisted of 84 animals in total, 40 in the experimental group and 44 in the control group. In all animals, three-walled bone defects measuring 0.5 × 0.4 × 0.5 cm3 were applied subperiosteally in the region of the angle of the lower jaw and filled in the experimental group using lyophilized gel mass of chitosan–alginate–hydroxyapatite (CH–SA–HA). In control animals, the bone cavities were filled with their own blood clots after bone trepanation and bleeding. The periods for monitoring bone regeneration were 3, 5, and 7 days and 2, 3, 4, 6, 8, and 10 weeks. The control of bone regeneration was carried out using multiple morphological and histological analyses. Results showed that the following process is an obligatory process and is accompanied by the binding and release of angiogenic implantation: the chitosan construct actively replaced early-stage defects with the formation of full-fledged new bone tissue compared to the control group. By the 7th day, morphological analysis showed that the formation of spongy bone tissue could be seen. After 2 weeks, there was a pronounced increase in bone volume (p < 0.01), and at 6 weeks after surgical intervention, the closure of the defect was 70–80%; after 8 weeks, it was 100% without violation of bone morphology with a high degree of mineralization. Thus, the use of modified chitosan after filling eliminates bone defects of critical size in the maxillofacial region, revealing early signs of bone regeneration, and serves as a promising material in reconstructive dentistry.

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Pathomorphism of Limb Major Vessels in Experimental Atherogenic Inflammation. The Role of Adventitial Intimal Relations (Review)

Cited by 5 publications

References 40 publications

Synthesis, Chemical and Biomedical Aspects of the Use of Sulfated Chitosan

Synthesis, Chemical and Biomedical Aspects of the Use of Sulfated Chitosan

The Role of Modified Chitosan in Bone Engineering in Diabetes Mellitus: Analytical Review

Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region

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