Pathology of infection caused by Dermatophilus-like organisms in porcine tonsils

Bak, U. B.; Azuma, Ryozo

doi:10.1016/s0021-9975(08)80193-4

Cited by 5 publications

(3 citation statements)

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“…suis' (Slack et al, 1975), differing completely from types 1 and 2. After a hiatus, pathological studies on swine tonsils were independently reopened and other actinomycotic lesions were discovered (Murakami et al, 1997), which differed from those of Tonsillophilus suis (Dermatophilus-like organisms) (Bak and Azuma, 1991;Murakami et al, 1991;Shiozawa et al, 1991). In these lesions, the microbial elements were detected immunohistochemically with antiserum of Actinomyces-like bacteria strain 101, which was isolated from piglet arthritis in 1971 (Murakami et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Department of Animal Health, Tokyo University of Agriculture, Tokyo, Japan

Murakami

Azuma

Oomi

et al. 1999

Journal of Veterinary Medicine Series A

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The present experiment was performed to test the pathogenicity of Actinomyces‐like bacteria in experimental animals and swine. Two rough (R) strains of Actinomyces‐like bacteria isolated from a site of arthritis and from the tonsil in pigs were used as inocula. To investigate their susceptibility to Actinomyces‐like bacteria, BALB/c, SS and ddY mice and guinea‐pigs were inoculated intraperitoneally with the strains of Actinomyces‐like bacteria. The ddY mice were used for the long‐term observation of Actinomyces‐like bacteria lesions and the mammary tissue of a sow was inoculated with Actinomyces‐like bacteria isolated from swine tonsil. Macroscopic observation revealed many abscesses on the surfaces of the abdominal and/or thoracic organs in the mice, but not on those of the guinea‐pigs. The sow developed firm nodules at the inoculation site in the mammary glands. Histopathologically, the lesions in the mice were characterized as actinomycotic abscesses in the early stage and as pus‐forming granuloma (PFG) in the advanced stage; the lesions were accompanied by crystalloid particles. Actinomyces‐like bacteria induced granulomatous mastitis in the sow, and the lesion was characterized as PFG. The characteristic actinomycotic lesions in swine mammary glands were reproduced by experimental infection.

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Section: Introductionmentioning

confidence: 99%

Department of Animal Health, Tokyo University of Agriculture, Tokyo, Japan

Murakami

Azuma

Oomi

et al. 1999

Journal of Veterinary Medicine Series A

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“…In 1971, a micro-organism resembling members of the genus Dermatophilus , strain W254 T , was isolated from an infected submandibular lymph node of a pig in Japan (Azuma & Bak 1980). In 1978 and 1979, we isolated 10 strains from infected swine tonsils in Korea and their similarity to strain W254 T was inferred from their morphological resemblance to this strain (Bak & Azuma, 1980, 1991). Six more strains were isolated from swine tonsils in Japan (Azuma et al , 1989).…”

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“…By cooperative pathological research, it became clear that the micro-organisms might preferably infect the swine tonsillar crypt (Bak & Azuma, 1991; Murakami et al , 1991). Also, it was revealed that the incidence of tonsil infection reached 10.5–57.1 % in fattening pigs (Bak & Azuma, 1980; Murakami et al , 1991) and breeding sows (Shiozawa et al , 1991).…”

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Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs

Azuma¹,

Ung-Bok²,

Murakami

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A micro-organism resembling members of the genus Dermatophilus, strain W254 T , which was isolated from the submandibular lymph node of a pig, and an additional 16 strains isolated from swine tonsils, were studied to establish their taxonomic status. Although all 17 strains were isolated anaerobically under an atmosphere of 100 % CO 2 , all of them were aerotolerant anaerobes. The micro-organisms showed at least five cellular morphologies: (i) a radially protrusive thallus, which proliferated into tuber-like cells; (ii) segmentation in both tubers and thallus followed by multilocule formation, (iii) development of coccoid forms in the locules; (iv) a change from the coccoid forms to zoospores; (v) resting cells, which were able to develop into protrusive thalli again. The microorganisms were positive for nitrate reduction, but negative for catalase, indole production, hydrolysis of urea and gelatin liquefaction. Milk was not decomposed and none of the strains was haemolytic. A total of 16 compounds, including glucose, were utilized as sole carbon sources and seven compounds, including L-arabinose, were not utilized. Three out of the 17 strains were subjected to further studies. The micro-organisms had meso-diaminopimelic acid in their peptidoglycan and galactose, glucose, madurose and a trace of mannose in their whole-cell sugar patterns. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol.Abbreviations: DAP, diaminopimelic acid; SEM, scanning electron microscopy; TEM, transmission electron microscopy. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Tonsilliphilus suis W254 T , HT1-19 and HT5-225 are AB096084, AB096085 and AB602380, respectively. A supplementary figure and a supplementary table are available with the online version of this paper.

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Msami

Khaschabi²,

Schöpf³

et al. 2001

Tropical Animal Health and Production

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When goats in Eastern Tanzania were screened for skin diseases, Dermatophilus congolensis was isolated from the skin lesions in 8 of 484 animals examined. In one severely affected case, the disease was also characterized by histological studies (Gram stain, Giemsa stain and routine HE studies) and electron microscopy. The histological picture was characterized by hyperkeratosis, parakeratosis, acanthosis, folliculitis and an inflammatory cellular reaction involving the epidermis. Gram stain and Giemsa stain revealed longitudinal and transverse branching filaments in the deeper layers of the epidermis. Ultrastructural studies demonstrated D. congolensis in various morphological forms, ranging from filamentous to tuber-shaped structures, mixed with numerous coccoid bodies of variable size. In some instances, the organisms were geometrically arranged in parallel rows of beading and were present in and among the degenerated epithelial cells. Several host cells showed degenerative changes. Ticks present on the goats were Amblyomma variegatum, Rhipicephalus evertsi, Rhipicephalus pravus and Boophilus sp. The clinical signs, pathological lesions, diagnosis, epidemiology and pathogenesis of the disease are discussed.

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Pathology of infection caused by Dermatophilus-like organisms in porcine tonsils

Cited by 5 publications

References 5 publications

Department of Animal Health, Tokyo University of Agriculture, Tokyo, Japan

Department of Animal Health, Tokyo University of Agriculture, Tokyo, Japan

Tonsilliphilus suis gen. nov., sp. nov., causing tonsil infections in pigs

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