Pathology of Bolivian Hemorrhagic Fever in the African Green Monkey

McLeod, Charles G.; Stookey, J. L.; White, James R.; Eddy, Gerald A.; Fry, George A

doi:10.4269/ajtmh.1978.27.822

Cited by 37 publications

(24 citation statements)

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“…During the development of this system, we also generated a minigenome (MG) reporter system, which allowed us to study the cis-and trans-acting factors required for MACV replication and gene expression in a BSL-2 environment without select agent restrictions, therefore enhancing our capabilities for potential virus replication studies and drug screening. And finally, we have identified a novel murine model of MACV-induced LNS, similar to what is described in reports of infected humans and NHPs (34)(35)(36). This is the first reported model of LNS in a murine species infected with a New World arenavirus.…”

supporting

confidence: 51%

“…challenge, but the disease development has not been well characterized (9,22). Studies utilizing "chaired" NHPs reported lethal disease development following intradermal, intramuscular, and intranasal challenge (34)(35)(36). Interestingly, African green monkeys, rhesus macaques, and cynomolgus monkeys developed a lethal late neurological syndrome (LNS), a disease also reported in guinea pig models infected with JUNV (37).…”

mentioning

confidence: 99%

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Rescue of a Recombinant Machupo Virus from Cloned cDNAs and In Vivo Characterization in Interferon (αβ/γ) Receptor Double Knockout Mice

Patterson

Seregin

Huang

et al. 2014

J Virol

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Machupo virus (MACV) is the etiological agent of Bolivian hemorrhagic fever (BHF), a reemerging and neglected tropical disease associated with high mortality. The prototypical strain of MACV, Carvallo, was isolated from a human patient in 1963, but minimal in vitro and in vivo characterization has been reported. To this end, we utilized reverse genetics to rescue a pathogenic MACV from cloned cDNAs. The recombinant MACV (rMACV) had in vitro growth properties similar to those of the parental MACV. Both viruses caused similar disease development in alpha/beta and gamma interferon receptor knockout mice, including neurological disease development and high mortality. In addition, we have identified a novel murine model with mortality and neurological disease similar to BHF disease reported in humans and nonhuman primates.

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supporting

confidence: 51%

mentioning

confidence: 99%

Rescue of a Recombinant Machupo Virus from Cloned cDNAs and In Vivo Characterization in Interferon (αβ/γ) Receptor Double Knockout Mice

Patterson

Seregin

Huang

et al. 2014

J Virol

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“…The survivors of the initial phase eventually went on to a second phase that included the central nervous system (CNS) disturbances and widespread infiltrates in a variety of tissues and organs. Similar findings were reported by Kastello et al 36 in their study of rhesus monkeys. These findings were consistent with Machupo infection in cynomologus monkeys.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 81%

“…All are associated with infection by a hantavirus. Yanagihara et al 39 reported mild, transient proteinuria and axotemia in three cynomolgus monkeys and chimpanzee (Pan troglodytes) following intravenous inoculation with Prospect Hill virus, a hantavirus isolated from meadow voles in the United States. Groen et al 40 reported that cynomolgus macaques were biting.…”

Section: Bunyavirusesmentioning

confidence: 99%

Vaccines against viral hemorrhagic fevers: Non-human primate models

Carrión

2011

Human Vaccines

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“…Beginning as early as day 26 and as late as day 40, most of the macaques surviving the first phase developed severe neurologic disease and succumbed to this ''second phase'' of disease. 9,25,30,40 In our experience with the Chicava strain, 1 macaque surviving the first phase of disease succumbed on day 34, during the second phase. The other 4 surviving macaques appeared clinically normal at the study end points, but some had immunohistochemical evidence of strong, specific, intracytoplasmic staining of neurons for MACV antigen in combination with chronic inflammation.…”

Section: Discussionmentioning

confidence: 96%

Pathology of Experimental Machupo Virus Infection, Chicava Strain, in Cynomolgus Macaques (Macaca fascicularis) by Intramuscular and Aerosol Exposure

Bell

Shaia²,

Bunton

et al. 2014

Vet Pathol

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Machupo virus, the causative agent of Bolivian hemorrhagic fever (BHF), is a highly lethal viral hemorrhagic fever of which little is known and for which no Food and Drug Administration-approved vaccines or therapeutics are available. This study evaluated the cynomolgus macaque as an animal model using the Machupo virus, Chicava strain, via intramuscular and aerosol challenge. The incubation period was 6 to 10 days with initial signs of depression, anorexia, diarrhea, mild fever, and a petechial skin rash. These were often followed by neurologic signs and death within an average of 18 days. Complete blood counts revealed leukopenia as well as marked thrombocytopenia. Serum chemistry values identified a decrease in total protein, marked increases in alanine aminotransferase and aspartate aminotransferase, and moderate increases in alkaline phosphatase. Gross pathology findings included a macular rash extending across the axillary and inguinal regions beginning at approximately 10 days postexposure as well as enlarged lymph nodes and spleen, enlarged and friable liver, and sporadic hemorrhages along the gastrointestinal mucosa and serosa. Histologic lesions consisted of foci of degeneration and necrosis/apoptosis in the haired skin, liver, pancreas, adrenal glands, lymph nodes, tongue, esophagus, salivary glands, stomach, small intestine, and large intestine. Lymphohistiocytic interstitial pneumonia was also present. Inflammation within the central nervous system (nonsuppurative encephalitis) was histologically apparent approximately 16 days postexposure and was generally progressive. This study provides insight into the course of Machupo virus infection in cynomolgus macaques and supports the usefulness of cynomolgus macaques as a viable model of human Machupo virus infection.

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Pathology of Bolivian Hemorrhagic Fever in the African Green Monkey

Cited by 37 publications

References 0 publications

Rescue of a Recombinant Machupo Virus from Cloned cDNAs and In Vivo Characterization in Interferon (αβ/γ) Receptor Double Knockout Mice

Rescue of a Recombinant Machupo Virus from Cloned cDNAs and In Vivo Characterization in Interferon (αβ/γ) Receptor Double Knockout Mice

Vaccines against viral hemorrhagic fevers: Non-human primate models

Pathology of Experimental Machupo Virus Infection, Chicava Strain, in Cynomolgus Macaques (Macaca fascicularis) by Intramuscular and Aerosol Exposure

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